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http://dx.doi.org/10.11626/KJEB.2022.40.3.341

Analysis of enzyme activity changes caused by flooding stress in upland crops  

Juhyung Shin (Department of Integrative Biology, Kyungpook National University)
Byeonggyu Kim (Department of Integrative Biology, Kyungpook National University)
Kihwan Kim (Department of Applied Biosciences, Kyungpook National University)
Tae-An Kang (Department of Applied Biosciences, Kyungpook National University)
Won-Chan Kim (Department of Integrative Biology, Kyungpook National University)
Publication Information
Korean Journal of Environmental Biology / v.40, no.3, 2022 , pp. 341-351 More about this Journal
Abstract
Among various environmental stresses, humid stress lacks mechanisms and biochemical understanding compared to drought, low temperature, and high salt stresses. The aim of this study was to investigate enzyme activity of field crops under humidity stress. Results of this study could be used as basic data for understanding humidity stress and early diagnosis. Growth and enzyme activities of sesame, perilla, red beans, sorghum, and beans as major field crops in Korea when flooded were investigated. It was confirmed that growths of both shoots and roots were retarded. In plants, anaerobic fermentation occurred due to flooding stress, which increased the activity of alcohol dehydrogenase (ADH) compared to the control group. Increases of reactive oxygen species (ROS) were also observed. All flooded plants showed increased peroxidase (POD) activity and lipid peroxidation. Their dyeing strength was darker than that of the control group, even in 3,3'-diaminobenzidine (DAB) staining. Since enzyme activity changes in plants appear relatively faster than changes in phenotype at the ground level, they could be used as biomarkers for early diagnosis of humidity stress in crops.
Keywords
field crops; flooding stress; biochemical change; diagnosis; mechanism;
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