Browse > Article
http://dx.doi.org/10.11626/KJEB.2021.39.1.006

Comparison of morphological characteristics of the river puffer, Takifugu obscurus, the tiger puffer, T. rubripes, their hybrids, and their triploid hybrids  

Park, In-Seok (Division of Convergence on Marine Science, College of Ocean Science and Engineering, Korea Maritime & Ocean University)
Oh, Ji Su (National Agency for Marine Seed Improvement, National Institute of Fisheries Science)
Publication Information
Korean Journal of Environmental Biology / v.39, no.1, 2021 , pp. 6-15 More about this Journal
Abstract
Various biometric and geometric measures were used to discriminate between the morphologically similar river puffer, Takifugu obscurus, tiger puffer, T. rubripes, their hybrids, and their triploid hybrids. The hybrids and triploid hybrids had greater anal fin width, nostril width, and snout length than the parental species (p<0.05). However, they had less caudal peduncle depth, inter-orbital width, head length, and head width(p<0.05). The morphometric and meristic characteristics of the hybrids and triploid hybrids were either intermediate between the parental species or more similar to those of one parental species. However, the external morphology of the hybrids and triploid hybrids was predominantly maternal. The triploid hybrids had asymmetry in the fin rays and gill raker numbers. This study identified phenotypic characteristics by distinguishing the morphological variables of river puffer, tiger puffer, their hybrids, and their triploid hybrids.
Keywords
hybrids; triploid hybrids; morphometric characteristic; river puffer(Takifugu obscurus); tiger puffer(T. rubripes);
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Albertson RC and TD Kocher. 2001. Assessing morphological differences in adaptive trait: a landmark-based morphometric approach. J. Exp. Zool. 289:385-403.   DOI
2 Bonar SA, GL Thomas and GB Pauley. 1988. Evaluation of the separation of triploid and diploid grass carp (Ctenopharyngodon idella) (Valenciennes), by external morphology. J. Fish. Biol. 33:895-898.   DOI
3 Cardin SX. 2000. Advances in morphometric identification of fishery stocks. Rev. Fish Biol. Fish. 10:91-112.   DOI
4 Chevassus B. 1983. Hybridization in fish. Aquaculture 33:245-262.   DOI
5 Kang HW, KB Shim, DY Kang, KC Jo, KC Song, JH Lee, HI Song, SG Son and YJ Cho. 2007. Sitological quality evaluation of cultured and wild river puffer, Takifugu obscurus. J. Aquacult. 20:147-153.
6 Kim JH, KT Son, JS Mok, EG Oh, JK Kim and TK Lee. 2006. Toxicity of the puffer fish Takifugu porphyreus and T. rubripes from coastal areas of Korea. Korean J. Fish. Aquat. Sci. 39:447-453.   DOI
7 Lim SG, MH Jeong, BS Kim, TH Lee, HW Gil and IS Park. 2016. Landmark-based morphometric and meristic analysis of Serranidae. Dev. Reprod. 20:73-85.   DOI
8 Nunez-Vazquez EJ, A Garcia-Ortega, AI Campa-Cordova, IA Parra, L Ibarra-Martinez, A Heredia-Tapia and JL Ochoa. 2012. Toxicity of cultured bullseye puffer fish, Sphoeroides annulatus. Mar. Drugs 10:329-339.   DOI
9 Park IS. 2020b. Comparative analysis of sectioned-body morphometric characteristics of diploid and triploid marine medaka Oryzias dancena. Korean J. Environ. Biol. 38:137-145.   DOI
10 Park IS, GC Choi and DS Kim. 1997. Production of hybrid and allotriploid between rainbow trout, Oncorhynchus mykiss and cherry salmon, O. masou. II. Characteristics of sex ratio and morphometric traits. J. Aquacult. 10:49-54.
11 Park IS, SY Lim, TH Lee, HW Gill and GY Yoo. 2017. Various characteristics of hybrid between river puffer, Takifugu obscurus and tiger puffer, T. rubripes, and their hybrid triploid. Dev. Reprod. 21:181-191.   DOI
12 Leary RF, FW Allendorf, KL Knudsen and GH Thorgaard. 1985. Heterozygosity and developmental stability in gynogenetic diploid and triploid rainbow trout. Heredity 54:219-225.   DOI
13 Dou D, X Wang, H Zhu, Y Bao, Y Wang, J Cui, X Meng, Y Zhu and X Qiu. 2019. The complete mitochondrial genome of the hybrid of Takifugu obscurus (♀)×Takifugu rubripes (♂). Mitochondrial DNA Part B-Resour. 4:3196-3197.   DOI
14 Benfey TJ. 1999. The physiology and behavior of triploid fishes. Fish. Sci. 7:39-67.
15 Caillon F, V Bonhomme, C Mollmann and R Frelat. 2018. A morphometric dive into fish diversity. Ecosphere 9:e02220.   DOI
16 Duncan DB. 1955. Multiple range and multiple F test. Biometrics 11:1-42.   DOI
17 Hamasaki M, Y Takeuchi, K Miyaki and G Yoshizaki. 2013. Gonadal development and fertility of triploid grass puffer, Takifugu niphobles induced by cold shock treatment. Mar. Biotechnol. 15:133-144.   DOI
18 Kasama M and H Kobayasi. 1991. Hybridization experiment between Gnathopogon elongatus elongatus (♀) and Carassius carassius (♂). Jpn. J. Ichthyol. 38:295-300.   DOI
19 Kim DS, YK Nam and IS Park. 1995. Survival and karyological analysis of reciprocal diploid and triploid hybrids between mud loach (Misgurnus mizolepis) and cyprinid loach (M. anguillicaudatus). Aquaculture 135:257-265.   DOI
20 Kotaro K and F Takeshi. 2007. Growth of tiger puffer, Takifugu rubripes at different salinities. J. World. Aquacult. Soc. 38:427-434.   DOI
21 Mojekwu TO and CI Anumudu. 2015. Advanced techniques for morphometric analysis in fish. J. Aquacult. Res. Dev. 6:354.
22 Yoo GY, TH Lee, HW Gill, SG Lim and IS Park. 2018. Cytogenetic analysis of hybrids and hybrid triploids between the river puffer, Takifugu obscurus and the tiger puffer, T. rubripes. Aquacult. Res. 49:637-650.   DOI
23 Park IS. 2019. A comparative analysis of cell cycles in diploid and induced triploid tissues in marine medaka (Oryzias dancena). Korean J. Environ. Biol. 37:735-740.   DOI
24 Park IS. 2020a. Morphometric characteristics of diploid and triploid Far Eastern catfish, Silurus asotus. Korean J. Environ. Biol. 38:106-113.   DOI
25 Park IS, HW Gil, JS Oh, HJ Choi and CH Kim. 2015. Comparative analysis of morphometric characteristics of Scorpaenidae and Gobioninae. Dev. Reprod. 19:85-96.   DOI
26 Dunham RA, RO Smitherman, MJ Brooks, M Benchakan and JA Chappell. 1982. Paternal predominance in reciprocal channelblue hybrid catfish. Aquaculture 29:389-396.   DOI
27 Rawat S, S Benakappa, J Kumar, ASK Naik, G Pandey and CW Pema. 2017. Identification of fish stocks based on truss morphometric: A review. J. Fish. Life Sci. 2:9-14.
28 Peruzzi S, B Chatain, E Saillant, P Haffray, B Menu and JC Falguiere. 2004. Production of meiotic gynogenetic and triploid sea bass, Dicentrarchus labrax L.: 1. Performances, maturation and carcass quality. Aquaculture 230:41-64.   DOI
29 Park IS and HW Gil. 2018. Comparative analysis of fluctuating asymmetry between ploidy and sex in marine medaka, Oryzias dancena. Dev. Reprod. 22:275-281.   DOI
30 Park IS, HW Gil and JS Oh. 2019. Changing salinity affects hematological and histological response in hybrids and hybrid triploids between river puffer, Takifugu obscurus and tiger puffer, T. rubripes. Dev. Reprod. 23:239-253.   DOI
31 Solar II, EM Donaldson and GA Hunter. 1984. Induction of triploidy in rainbow trout (Salmo gairdneri Richardson) by heat shock, and investigation of early growth. Aquaculture 42:57-67.   DOI
32 Strussmann CA and F Takashima. 1993. Hepatocyte nuclear size and nutritional condition of larval pejerrey (Odontesthes bonariensis)(Cuvier et Valenciennes). J. Fish. Biol. 36:59-65.   DOI
33 Wilkins NP, E Gosling, A Curatolo, A Linnane, C Jordan and HP Courtney. 1995. Fluctuating asymmetry in Atlantic salmon, European trout and their hybrids, including triploids. Aquaculture 137:77-85.   DOI
34 Thorgaard GH, PS Rabinovitch, MW Shen, GAE Gall, J Propp and FM Utter. 1982. Triploid rainbow trout identified by flow cytometry. Aquaculture 29:305-309.   DOI
35 Park IS, YK Nam and DS Kim. 2006. Growth performance, morphometric trait and gonad development of induced reciprocal diploid and triploid hybrids between the mud loach(Misgurnus mizolepis) and cyprinid loach (Misgurnus anguillicaudatus). Aquacult. Res. 37:1246-1253.   DOI
36 Bonnet S, P Haffray, JM Blanc, F Vallee, C Vauchez, A Faure and B Fauconneau. 1999. Genetic variation in growth parameters until commercial size in diploid and triploid freshwater rainbow trout(Oncorhynchus mykiss) and seawater brown trout (Salmo trutta). Aquaculture 173:359-375.   DOI
37 Strauss RE and CE Bond. 1990. Taxonomic methods: Morphology. pp. 125-130. In: Methods for Fish Biology (Schreck CB and PB Moyle eds.). American Fisheries Society. Bethesda, MD.
38 Strauss RE and FL Bookstein. 1982. The truss: body from reconstructions in morphometrics. Syst. Zool. 31:113-135.   DOI
39 Scheerer PD and GH Thorgaard. 1983. Increased survival in salmonid hybrids by induced triploidy. Can. J. Fish. Aquat. Sci. 40:2040-2044.   DOI
40 Gray AK, MA Evans and GH Thorgaard. 1993. Viability and development of diploid and triploid salmonid hybrids. Aquaculture 112:125-142.   DOI
41 Turan C. 1999. A note on the examination of morphometric differentiation among fish populations: The truss system. Turk. J. Zool. 23:259-263.
42 Yang Z and Y Chen. 2003. Length-weight relationship of obscure puffer (Takifugu obscurus) during spawning migration in the Yangtze River, China. J. Freshw. Ecol. 18:349-352.   DOI
43 Park IS and CI Zhang. 1994. Morphometrical differences between diploid and induced triploid cherry salmon, Oncorhynchus masou. Korean J. Ichthyol. 6:206-221.
44 Normala J, AA Mohd, MAB Abol, AA Nur, W Khor, TV Okomoda and MS Shahreza. 2017. Morphometric variations between triploid and diploid Clarias gariepinus(Burchell, 1822). Croat. J. Fish. 75:113-121.   DOI