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http://dx.doi.org/10.11626/KJEB.2020.38.2.248

A flowcytometric determination of DNA content in Pacific abalone, Haliotis discus hannai cell  

Park, In-Seok (Division of Marine Bioscience, Korea Maritime & Ocean University)
Publication Information
Korean Journal of Environmental Biology / v.38, no.2, 2020 , pp. 248-253 More about this Journal
Abstract
The level at which analyses of DNA content might contribute more significantly to the genetic mechanisms of evolution lies in the events of speciation. The object of this study was to investigate the DNA content of abalone (Haliotis discus hannai) and determine the optimal tissue samples for measuring the DNA content of abalone by flowcytometry without fixation. The DNA content (pg/nucleus) of gill tissue (2.5±0.08), which was contaminated with protozoa, was significantly lower than that of muscle tissue (3.2±0.02), mantle tissue (3.2±0.02) (p<0.05), and a standard reference standard, while the DNA contents of muscle tissue and mantle tissue were higher than that of the standard reference. Considering the results of this study, DNA content analysis with flowcytometry is an acute and rapid method by which muscle tissue and mantle tissue are the most appropriate sample for measuring the DNA content of abalone without fixation.
Keywords
abalone (Haliotis discus hannai); DNA content; flowcytometry; tissues;
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Times Cited By KSCI : 10  (Citation Analysis)
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1 Park IS. 2019. A comparative analysis of cell cycles in diploid and induced triploid tissues in marine medaka (Oryzias dancena). Korean J. Environ. Biol. 37:735-740.   DOI
2 Park IS. 2020a. Morphometric characteristics of diploid and triploid Far Eastern catfish, Silurus asotus. Korean J. Environ. Biol. 38:106-113.   DOI
3 Park IS. 2020b. Comparative analysis of sectioned-body morphometric characteristics of diploid and triploid marine medaka, Oryzias dancena. Korean J. Environ. Biol. 38:137-145.   DOI
4 Park IS, CH Kim and DS Kim. 1999. Karyotypes and cellular DNA contents of two species in the genus Lateolabrax from Korea. Fish. Sci. 65:488-489.   DOI
5 Thorgaard GH, PS Rabinovitch, MW Shen, GAE Gall, J Propp and FM Utter. 1982. Triploid rainbow trout identified by flow cytometry. Aquaculture 29:305-309.   DOI
6 Vanparys C, M Maras, M Lenjou, J Robbens, D van Bockstaele, R Blust and W De Coen. 2006. Flow cytometric cell cycle analysis allows for rapid screening of estrogenicity in MCF-7 breast cancer cells. Toxicol. Vitro 20:1238-1248.   DOI
7 Wolters WR, CL Chrisman and GS Libey. 1982. Erythrocyte nuclear measurements of diploid and triploid channel catfish, Ictalurus punctatus (Rafinicsque). J. Fish Biol. 20:253-258.   DOI
8 Da Silva PM and A Villalba. 2004. Comparison of microscopic techniques for the diagnosis of the infection of the European flat oyster Ostrea edulis by the protozoan Bonamia ostreae. J. Invertebr. Pathol. 85:97-104.   DOI
9 An HS, YJ Jee, SJ Han, BL Kim, EM Kim and IS Park. 2007. Induction of a new hybrid between Haliotis gigantea (female) and H. discus (male). Korean J. Genetics 29:239-244.
10 Ashton-Alcox KA, B Allam and SE Ford. 2001. Application of flowcytometry to bivalve pathology. pp. 85-124. In: Recent Advances in Marine Biotechnology, Vol. 5 (Fingerman M and R Nagabhushanam eds.). Science Publishers, Plymouth, UK.
11 Estevam J, H Danaee, R Liu, J Ecsedy, W Trepicchio and T Wyant. 2011. Validation of a flow cytometry based G2M delay cell cycle assay for use in evaluating the pharmacodynamic response to Aurora A inhibition. J. Immunol. Methods 363: 135-142.   DOI
12 FAO. 2017. Abalone production continues to grow, coupled with continuing demand, price high and stable. GLOBEFISH-Information and Analysis on World Fish Trade. Food and Agriculture Organization of the United Nations. Rome, Italy.
13 Kim HS, KW Lee, HS Jeong, J Kim, AY Yun, SH Cho, GA Lee and KY Kim. 2017. Effect of dietary microalgae, diatom-dominant, oil extracts on growth, body composition and shell color of juvenile abalone Haliotis discus. Korean J. Fish. Aquat. Sci. 50:738-744.   DOI
14 Gonzalez-Tizon A, A Martinez-Lage, I Rego, J Ausio and J Mendez. 2000. DNA contents, karyotypes and chromosomal location of 18S-5.8S-28S ribosomal loci in some species of bivalve molluscs from the Pacific Canadian coast. Genome 43: 1065-1072.   DOI
15 Hinegardner R. 1974. Cellular DNA content of the mollusca. Comp. Biochem. Physiol. 47:447-460.   DOI
16 Jee YJ and YJ Chang. 2012. Cytogenetic analysis of the triploid Pacific abalone, Haliotis discus hannai. Korean J. Malacol. 28:37-43.   DOI
17 NFFC. 2000. Marine Products in Korea. National Federation of Fisheries Cooperatives. Suhyup Publ. Co., Seoul, Korea.
18 Cho JH, HG Nam and KS Oh. 2018. Processing and quality characteristics of a cultured recessive small-sized abalone Haliotis discus hannai extract. Korean J. Fish. Aquat. Sci. 51:640-646.   DOI