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http://dx.doi.org/10.11626/KJEB.2018.36.4.601

Application of Antimicrobial Peptides against Microcystis aeruginosa to Control Harmful Algal Blooms  

Han, Sang-Il (Division of Environmental Science & Ecological Engineering, Korea University)
Park, Yoonkyung (Research Center for Proteinaceous Materials (RCPM), Chosun University)
Choi, Yoon-E (Division of Environmental Science & Ecological Engineering, Korea University)
Publication Information
Korean Journal of Environmental Biology / v.36, no.4, 2018 , pp. 601-609 More about this Journal
Abstract
Microcystis aeruginosa, a freshwater cyanobacteria species known to be one of the most predominant species responsible for cyanobacterial harmful algal blooms (CyanoHABs). It has been frequently associated with the contamination of neurotoxins and peptide hepatotoxins, such as microcystin and lipopolysaccharides-LPSs. CyanoHABs control technologies so far put in place do not provide a fundamental solution and cause secondary pollution linked with the control measures. For this study, algicidal peptides, which have been reported to be non-toxic and to have antimicrobial properties, were employed for the development of novel eco-friendly control against CyanoHABs. The four peptides (CMA1, CMA2, HPA3P, and HPA3NT3) selected in this study showed significant algicidal effects against M. aeruginosa cells inducing cell aggregation and flotation. Moreover, the newly generated peptides (K160242-5) with certain modifications also displayed high algicidal activity. The algicidal activity of the peptides was found to depend on the concentrations and structures of each of amino acid. The results of this study suggested a novel possibility of CyanoHABs control using the non-toxic algicidal peptides.
Keywords
Microcystis aeruginosa; cyanobacterial blooms; HABs; algicidal peptide; antimicrobial peptide;
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  • Reference
1 Anderson DM. 2009. Approaches to monitoring, control and management of harmful algal blooms (HABs). Ocean Coastal Manage. 52:342-347.
2 Briand E, M Bormans, M Gugger, PC Dorrestein and WH Gerwick. 2016. Changes in secondary metabolic profiles of Microcystis aeruginosa strains in response to intraspecific interactions. Environ. Microbiol. 18:384-400.   DOI
3 Chen Y, J Li, J Wei, A Kawan, L Wang and X Zhang. 2017. Vitamin C modulates Microcystis aeruginosa death and toxin release by induced Fenton reaction. J. Hazard. Mater. 321:888-895.
4 Da Ros PC, CS Silva, ME Silva-Stenico, MF Fiore and HF de Castro. 2012. Microcystis aeruginosa lipids as feedstock for biodiesel synthesis by enzymatic route. J. Mol. Catal. B-Enzym. 84:177-182.
5 Dolah FMV, D Roelke and RM Greene. 2001. Health and ecological impacts of harmful algal blooms: risk assessment needs. Hum. Ecol. Risk Assess. 7:1329-1345.
6 Gumbo RJ, G Ross and ET Cloete. 2008. Biological control of Microcystis dominated harmful algal blooms. Afr. J. Biotechnol. 7:4765-4773.
7 Hadjoudja S, V Deluchat and M Baudu. 2010. Cell surface characterisation of Microcystis aeruginosa and Chlorella vulgaris. J. Colloid Interface Sci. 342:293-299.
8 Karlson AM and R Mozuraitis. 2011. Deposit-feeders accumulate the cyanobacterial toxin nodularin. Harmful Algae 12:77-81.
9 Lee DG, DH Kim, Y Park, HK Kim, HN Kim, YK Shin, CH Choi and KS Hahm. 2001a. Fungicidal effect of antimicrobial peptide, PMAP-23, isolated from porcine myeloid against Candida albicans. Biochem. Biophys. Res. Commun. 282:570-574.
10 Lee JK, CH Seo, T Luchian and Y Park. 2015. The antimicrobial peptide CMA3 derived from the CA-MA hybrid peptide: antibacterial and anti-inflammatory activities with low cytotoxicity and mechanism of action in Escherichia coli. Antimicrob. Agents Chemother. 60:495-506.
11 Lee JK, SC Park, KS Hahm and Y Park. 2013. Antimicrobial HPA3NT3 peptide analogs: placement of aromatic rings and positive charges are key determinants for cell selectivity and mechanism of action. Biochim. Biophys. Acta-Biomembr. 1828:443-454.
12 Lee TH, KN Hall, MJ Swann, JF Popplewell, S Unabia, Y Park, KS Hahm and MI Aguilar. 2010. The membrane insertion of helical antimicrobial peptides from the N-terminus of Helicobacter pylori ribosomal protein L1. Biochim. Biophys. Acta-Biomembr. 1798:544-557.
13 Lee T, K Nakano and M Matsumara. 2001b. Ultrasonic irradiation for blue-green algae bloom control. Environ. Technol. 22:383-390.
14 Liu Y, S Chen, J Zhang, X Li and B Gao. 2017. Stimulation effects of ciprofloxacin and sulphamethoxazole in Microcystis aeruginosa and isobaric tag for relative and absolute quantitation-based screening of antibiotic targets. Mol. Ecol. 26:689-701.
15 Ma J, JD Brookes, B Qin, HW Paerl, G Gao, P Wu, W Zhang, J Deng, G Zhu, Y Zhang, H Xu and H Niu. 2014. Environmental factors controlling colony formation in blooms of the cyanobacteria Microcystis spp. in Lake Taihu, China. Harmful Algae 31:136-142.
16 Mereuta L, T Luchian, Y Park and KS Hahm. 2008. Single-molecule investigation of the interactions between reconstituted planar lipid membranes and an analogue of the HP (2-20) antimicrobial peptide. Biochem. Biophys. Res. Commun. 373:467-472.
17 Paerl HW, H Xu, MJ McCarthy, G Zhu, B Qin, Y Li and WS Gardner. 2011. Controlling harmful cyanobacterial blooms in a hyper-eutrophic lake (Lake Taihu, China): the need for a dual nutrient (N & P) management strategy. Water Res. 45:1973-1983.
18 Olli K, R Klais and T Tamminen. 2015. Rehabilitating the cyanobacteria-niche partitioning, resource use efficiency and phytoplankton community structure during diazotrophic cyanobacterial blooms. J. Ecol. 103:1153-1164.
19 Paerl HW and TG Otten. 2013. Harmful cyanobacterial blooms: causes, consequences, and controls. Microb. Ecol. 65:995-1010.   DOI
20 Paerl HW and VJ Paul. 2012. Climate change: links to global expansion of harmful cyanobacteria. Water Res. 46:1349-1363.   DOI
21 Park SC, JK Lee, SW Kim and Y Park. 2011. Selective algicidal action of peptides against harmful algal bloom species. PLoS One 6:e26733.
22 Qian H, X Pan, J Chen, D Zhou, Z Chen, L Zhang and Z Fu. 2012. Analyses of gene expression and physiological changes in Microcystis aeruginosa reveal the phytotoxicities of three environmental pollutants. Ecotoxicology 21:847-859.
23 Ren H, P Zhang, C Liu, Y Xue and B Lian. 2010. The potential use of bacterium strain R219 for controlling of the bloom-forming cyanobacteria in freshwater lake. World J. Microbiol. Biotechnol. 26:465-472.
24 Schopf JW. 2000. The fossil record: tracing the roots of the cyanobacterial lineage. pp. 13-35. In The Ecology of Cyanobacteria. Springer, Dordrecht.
25 Stanier R, R Kunisawa, M Mandel and G Cohen-Bazire. 1971. Purification and properties of unicellular blue-green algae (order Chroococcales). Bacteriol. Rev. 35:171.
26 Wei Y, J Niu, L Huan, A Huang, L He and G Wang. 2015. Cell penetrating peptide can transport dsRNA into microalgae with thin cell walls. Algal Res. 8:135-139.
27 Sun LW, WJ Jiang, H Sato, M Kawachi and XW Lu. 2016. Rapid classification and identification of Microcystis aeruginosa strains using MALDI-TOF MS and Polygenetic Analysis. PLoS One. 11:e0156275.
28 Suresh A and YC Kim. 2013. Translocation of cell penetrating peptides on Chlamydomonas reinhardtii. Biotechnol. Bioeng. 110:2795-2801.
29 Vives E, P Brodin and B Lebleu. 1997. A truncated HIV-1 Tat protein basic domain rapidly translocates through the plasma membrane and accumulates in the cell nucleus. J. Biol. Chem. 272:16010-16017.