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http://dx.doi.org/10.11626/KJEB.2018.36.4.463

Genetic Difference Analysis and Environmental Assessment of Miscanthus sinensis and Phragmites australis to Apply Regional Seed for Restoration in Korea  

Hong, Sun Hee (Department of Plant Life & Environment Science, College of Agriculture & Life Science, Hankyoung National University)
Park, Sang Yong (Department of Plant Resources, College of Industrial Science, Kongju National University)
Min, Kyeoung Do (Department of Plant Resources, College of Industrial Science, Kongju National University)
Kim, Jae Yoon (Department of Plant Resources, College of Industrial Science, Kongju National University)
Publication Information
Korean Journal of Environmental Biology / v.36, no.4, 2018 , pp. 463-470 More about this Journal
Abstract
Restoration ecology is the practical study of renewing and restoring a spoilt, degraded, or devastated ecosystems in the environment. Because the Korean industry has been drastically developed for the past few decades, the Korean ecosystem requires restoration using regional seed. In this study, we identified the variation of phylogenic relationship of Miscanthus sinensis or Phragmites australis by locations in Korea. Chloroplast DNA atpF-H and psbA-trnH interspace region were used as a molecular marker to resolve the phylogenic relationship in 10 different locations. We performed the molecular phylogenetic analysis with 10 chloroplast DNAs from each location using Kimura 2-parameter. The analysis of Miscanthus showed that all atpF-H genes were exact matches except for Ose san. In contrast to Mischanthus, the atpF-H genes from Phragmites were observed to have more variation. A total of 7 locations revealed the variation in chloroplast gene. According to the phylogenic tree in Phragmites, 2 of 10 samples in 6 locations and 3 of 10 in 1 location showed variation with 0.160-0.181 genetic distance. According to the genetic distance of the Miscanthus sinensis, there were no mutations in all regions except the Hongsung. These results support regional differences and show the necessity for seed collection by region. In the case of Phragmites australis, genetic variation occurred in all regions.
Keywords
Miscanthus sinensis; Phragmites australis; ecological restoration; genetic difference; molecular marker;
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