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http://dx.doi.org/10.11626/KJEB.2015.33.4.450

The Expression of Hsp70 and GST Genes in Mytilus coruscus Exposed to Water Temperature and Salinity  

Kim, Chul Won (Department of Aquaculture, Korea National College of Agriculture and Fisheries)
Kang, Han Seung (MS BioLab)
Publication Information
Korean Journal of Environmental Biology / v.33, no.4, 2015 , pp. 450-458 More about this Journal
Abstract
The heat shock proteins (Hsps), one of the most highly conserved groups of proteins, play crucial roles in protecting cells against environmental stressors, such as temperature, salinity, heavy metals and pathogenic bacteria. The glutathione S-transferases (GST) have important role in detoxification of oxidative damage, environmental chemicals and environmental stress. The purpose of this study is to investigate the gene expression of Hsp70 and GST on change of temperature and salinity in Mytilus coruscus. The M. coruscus was cultured in incubator of separate temperature and salinity (8, 20, $30^{\circ}C{\times}20$‰, 25‰, 30‰) for 28 days. Ten individuals in each group were selected after each 14 and 28 days exposure. Results that the expression of Hsp70 mRNA was no significant changed in M. coruscus exposed to temperature ($8^{\circ}C$, $20^{\circ}C$, $30^{\circ}C$) and salinity (20‰, 25‰, 30‰) for 14 days. Whereas the expression of Hsp70 mRNA was increased in exposure to temperature $30^{\circ}C$ and salinity (20‰, 25‰, 30‰) for 28 days. The expression of GST mRNA was increased in exposure to temperature $30^{\circ}C$, salinity (25‰, 30‰) for 14 days and temperature ($8^{\circ}C$, $20^{\circ}C$, $30^{\circ}C$), salinity (20‰, 25‰, 30‰) for 28 days. These results suggest that Hsp70 and GST were played roles in biomarker gene on the thermal and salinity stress.
Keywords
Mytilus coruscus; Hsp70; GST; temperature; salinity;
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1 Paul AJ and JM Paul. 1998. Respiration rate and thermal tolerances of pinto abalone Haliotis kamtschatkana. J. Shellfish Res. 17:743-745.
2 Shin YK and CH Wi. 2004. Effect of temperature and salinity on survival and metabolism of the hard shelled mussel Mytilus coruscus, Bivalve: Mytilidae. J. Aquacult. 17:103-108.
3 Werner I. 2004. The influence of salinity on the heat-shock protein response of Potamocorbula amurensis (Bivalvia). Mar. Environ. Res. 58:803-807.   DOI
4 Wi CH, YJ Chang, SJ Lee, YB Hur and JS Lee. 2003. Sexual maturation and gametogenic cycle of the Hard Shelled Mussel, Mytilus coruscus (Bivalvia: Mytilidae). J. Aquacult. 16:245-251.
5 Xu Q and Y Qin. 2012. Molecular cloning of heat shock protein 60 (PtHSP60) from Portunus trituberculatus and its expression response to salinity stress. Cell Stress Chaperone. 17:589-601.   DOI
6 Yoo SK, KH Kang and DY Lee. 1988. Occurrence and survival rate of the larvae of sea mussel, Mytilus edulis. Korean J. Fish. Aquat. Sci. 21:35-41.
7 You L, X Ning, F Liu, J Zhao, Q Wang and H Wu. 2013. The response profiles of HSPA12A and TCTP from Mytilus galloprovincialis to pathogen and cadmium challenge. Fish Shellfish Immunol. 35:343-350.   DOI
8 Colinet H, SF Lee and A Hoffmann. 2010. Temporal expression of heat shock genes during cold stress and recovery from chill coma in adult Drosophila melanogaster. FEBS J. 277:174-185.   DOI
9 Fangue NA, M Hofmeister and PM Schulte. 2006. Intraspecific variation in thermal tolerance and heat shock protein gene expression in common killifish, Fundulus heteroclitus. J. Exp. Biol. 209:2859-2872.   DOI
10 Feder ME and GE Hofmann. 1999. Heat-shock proteins, molecular chaperones, and the stress response: evolutionary and ecological physiology. Annu. Rev. Physiol. 61:243-282.   DOI
11 Ferraris M, S Radice, P Catalani, M Francolini, L Marabini and E Chiesara. 2002. Early oxidative damage in primary cultured trout hepatocytes: a time course study. Aquat. Toxicol. 50:283-296.
12 Georgopoulos C and W Welch. 1993. Role of the major heat shock proteins as molecular chaperones. Annu. Rev. Cell Biol. 9:601-634.   DOI
13 Hamed RR, NM Farid, SE Elowa and AM Abdalla. 2003. Glutathione related enzyme levels of freshwater fish as bioindicators of pollution. Environmentalist 23:313-322.   DOI
14 Hansson T, D Schiedek, KK Lehtonen, PJ Vuorinen, Liewenborg, E Noaksson, U Tjarnlund, M Hansson and L Balk. 2006. Biochemical biomarkers in adult female perch (Perca fluviatilis) in a chronically polluted gradient in the Stockholm recipient (Sweden). Marine Poll. Bull. 53:451-468.   DOI
15 Hayes JD and DJ Pulford. 1995. The glutathione S-transferase supergene family: regulation of GST and the contribution of the isoenzymes to cancer chemprotection and drug resistance. Crit. Rev. Biochem. Mol. Biol. 30:445-600.   DOI
16 Kim TH, KJ Kim, MK Choi and IK Yeo. 2006. Physiological changes of juvenile abalone, Haliotis sieboldii exposed to acute water-temperature stress. J. Aquacult. 77-83.
17 Hayes JD, JU Flanagan and IR Jowsey. 2005. Glutathione transferases. Annu. Rev. Pharmacol. Toxicol. 45:51-88.   DOI
18 Hu F, L Pan, M Xiu, Q Jin G Wang and C Wang. 2015. Bioaccumulation and detoxification responses in the scallop Chlamys farreri exposed to tetrabromobisphenol A (TBBPA). Environ. Toxicol. Pharmacol. 39:997-1007.   DOI
19 Hughes VF, AK Trull, A Gimson, PJ Friend, N Jamieson, A Duncan, DG Wight, AT Prevost and GJ Alexander. 1997. Randomized trial to evaluate the clinical benefits of serum alpha-glutathione S-transferase concentration monitoring after liver transplantation. Transplantation 64:1446-1452.   DOI
20 Kregel KC. 2002. Heat shock proteins: modifying factors in physiological stress responses and acquired thermotolerance. J. Appl. Physiol. 92:2177-2186.   DOI
21 Lane DJW, AR Beaumont and JR Hunter. 1985. Byssus drifting and the drifting threads of the young post larval mussel Mytilus edulis. Mar. Biol. 84:301-308.   DOI
22 Lau PS and HL Wong. 2003. Effect of size, tissue and location on six biochemical markers in the greenlipped mussel, Perna viridis. Mar. Pollut. Bull. 46:1563-1572.   DOI
23 Liu D, L Pan, Z Li, Y Cai and J Miao. 2014a. Metabolites analysis, metabolic enzyme activities and bioaccumulation in the clam Ruditapes philippinarum exposed to benzo(a)pyrene. Ecotoxicol. Environ. Saf. 107:251-259.   DOI
24 Loomis SH, AD Ansell, RN Gibson and M Barnes. 1995. Freezing tolerance of marine invertebrates. Oceanogr. Mar. Biol. Annu. Rev. 33:337-350.
25 Bailey J, J Parsons and CA Couturier. 1996. Salinity tolerance in the blue mussel, Mytilus edulis. Bull. Aquacult. Assoc. Can. 96:74-76.
26 Chapple JP, GR Smerdon and AJS Hawkins. 1997. Stress-70 protein induction in Mytilus edulis: Tissue-specific responses to elevated temperature reflect relative vulnerability and physiological function. J. Exp. Mar. Biol. Ecol. 217:225-235.   DOI
27 Chapple JP, GR Smerdon, RJ Berry and AJS Hawkins. 1998. Seasonal changes in stress-70 protein levels reflect thermal tolerance in the marine bivalve Mytilus edulis L. J. Exp. Mar. Biol. Ecol. 229:53-68.   DOI
28 Liu H, J He, C Chi and J Shao. 2014b. Differential HSP70 expression in Mytilus coruscus under various stressors. Gene 543:166-173.   DOI
29 Loguercio C, N Caporaso, C Tuccillo, F Morisco, G Del Vecchio Blanco and C Del Vecchio Blanco. 1998. Alpha-glutathione transferases in HCV-related chronic hepatitis: a new predictive index of response to interferon therapy? J. Hepatol. 28:390-395.   DOI
30 Luca-Abbott SB, BJ Richardson, KE McClellan, GJ Zheng, M Martin and PKS Lam. 2005. Field validation of antioxidant enzyme biomarkers in mussels (Perna viridis) and clams (Ruditapes philippinarum) transplanted in Hong Kong coastal waters. Mar. Pollut. Bull. 51:694-707.   DOI
31 Myrnes B and IW Nilsen. 2007. Glutathione S-transferase from the Icelandic scallop (Chlamys islandica): isolation and partial characterization. Comp. Biochem. Physiol. C, Toxicol. Pharmacol. 144:403-407.   DOI
32 Nam BH, EM Park, YO Kim, DG Kim, YJ Jee, SJ Lee and CM An. 2013. Analysis of heat, cold or salinity stress-inducible genes in the Pacific abalone, Haliotis discus hannai, by suppression subtractive hybridization. Korean J. Malacol. 29:181-187.   DOI
33 Newell RC and LH Kofoed. 1977. Adjustment of the components of energy balance in the gastropod Crepidula fornicate in response to thermal acclimation. Mar. Biol. 44:275-286.   DOI
34 Parsell D and S Lindquist. 1993. The function of heat-shock proteins in stress tolerance: degradation and reactivation of damaged proteins. Annu. Rev. Genet. 27:437-496.   DOI