Browse > Article

Dynamics of Bacterial Communities Analyzed by DGGE during Cyanobacterial Bloom in Daechung Reservoir, Korea  

Ko, So-Ra (Environmental Biotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology)
Ahn, Chi-Yong (Environmental Biotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology)
Lee, Young-Ki (Environmental Biotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology)
Oh, Hee-Mock (Environmental Biotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology)
Publication Information
Korean Journal of Environmental Biology / v.29, no.3, 2011 , pp. 225-235 More about this Journal
Abstract
The change of microbial communities during cyanobacterial bloom was comparatively analyzed by 16S rDNA PCR-DGGE in Daechung Reservoir during 2003~2005. Morphological analysis showed that Cyanophyceae dominated algal community in the bloom. Dominant cyanobacteria were Microcystis, Planktothrix (Oscillatoria), Phormidium and Anabaena. We used 16S rDNA-denaturing gradient gel electrophoresis (DGGE) profiles and phylogenetic affiliations of the DGGE bands to analyze the community structure and diversity of the predominant microbial community. The DGGE band patterns demonstrated that the most frequent bands were identified as Microcystis during the monitoring periods, Planktothrix also dominated on September 2003 and 2004, whereas Anabaena was showed a peak on September 2005 and Aphanizomenon on August 2003. DGGE and phylogenetic analysis provided us new information that could not be obtained by traditional, morphological analysis. The relationship between cyanobacteria and other aquatic bacteria can be traced and their genetic diversity also identified in detail.
Keywords
algal bloom; community; cyanobacteria; DGGE;
Citations & Related Records
Times Cited By KSCI : 9  (Citation Analysis)
연도 인용수 순위
1 Kanoshina I, U Lips and JM Leppanen. 2003. The influence of weather conditions (temperature and wind) on cyanobacterial bloom development in the Gulf of Finland (Baltic Sea). Harmful Algae 2:29-41.   DOI   ScienceOn
2 Kim SG, SK Rhee, CY Ahn, SR Ko, GG Choi, JW Bae, YH Park and HM Oh. 2006. Determination of cyanobacterial diversity during algal blooms in Daechung Reservior, Korea, on the basis of cpcBA intergenic spacer region analysis. Appl. Environ. Microbiol. 72:3252-3258.   DOI   ScienceOn
3 Kirchman D. 2002. The ecology of Cytophaga-Flavobacteria in aquatic ecosystem. FEMS Microbiol. Ecol. 39:91-100.
4 Menzel DW and N Corwin. 1965. The measurement of total phosphorus in seawater based on the liberation of organically bound fractions by persulfate oxidation. Limnol. Oceanogr. 10:280-282.   DOI   ScienceOn
5 Muyzer G, EC de Waal and AG Uitterlinden. 1993. Profiling of complex microbial populations by denaturing gradient gel eletrophoresis analysis of polymerase chain reactionamplified genes coding for 16S rRNA. Appl. Environ. Microbiol. 59:695-700.
6 Nergo AI, CD Hoyos and JC Vega. 2000. Phytoplankton structure and dynamics in Lake Sanabria and Valparaiso reservoir (NW Spain). Hydrobiologia 424:25-37.   DOI   ScienceOn
7 Shunyu S, L Yongding, S Yinwu, L Genbao and L Dunhai. 2006. Lysis of Aphanizomenon flos-aquae (Cyanobacterium) by a bacterium Bacillus cereus. Biol. Control 39:345-351.   DOI   ScienceOn
8 Wood LW. 1985 Chloroform-methanol extraction of chlorophyll-a. Can. J. Fish. Aquat. Sci. 42:38-43.   DOI
9 Demergasso C, EO Casamayor, G Chong, P Galleguillos, L Escudero and C Pedros-Alio. 2004. Distribution of prokaryotic genetic diversity in athalassohaline lakes of the Atacama Desert, Northern Chile. FEMS Microbiol. Ecol. 48:57-69.   DOI   ScienceOn
10 Diez B, C Pedros-Alio, TL Marsh and R Massana. 2001. Application of Denaturing Gradient Gel Electrophoresis (DGGE) to study the diversity of marine picoeukaryotic assamblages and comparison of DGGE with other molecular techniques. Appl. Environ. Microbiol. 67:2942-2951.   DOI   ScienceOn
11 Felip M and J Catalan. 2000. The relationship between phytoplankton biovolume and chlorophyll in a deep oligotrophic lake: decoupling in their spatial and temporal maxima. J. Plankton Res. 22:91-105.   DOI
12 Jaspers E, K Nauhaus, H Cypionka and J Overmann. 2001. Multitude and temporal variability of ecological niches as indicated by the diversity of cultivated bacterioplankton. FEMS Microbiol. Ecol. 36:153-164.   DOI
13 Felske A, ADL Akkermans and WM de Vos. 1998. Quantification of 16S rRNAs in complex bacterial communities by multiple competitive reverse transcription PCR in temperature gradient gel electrophoresis fingerprints. Appl. Environ. Microbiol. 64:4581-4587.
14 Gucht KV de, K Sabbe, L De Meester, N Vloemans, G Zwart, M Gillis and W Vverman. 2001. Contrasting bacterioplankton community composition and seasonal dynamics in two neighbouring hypertrophic freshwater lakes. Environ. Microbiol. 3:680-690.   DOI   ScienceOn
15 Ishii K and M Fukui. 2001. Optimization of annealing temperature to reduce bias caused by a primer mismatch in multitemplate PCR. Appl. Environ. Microbiol. 67:3753-3755.   DOI   ScienceOn
16 이영옥, 이희순. 2002. 농업용 저수지에서의 질화세균의 계절적인 변화. 한국육수학회지. 35:152-159.
17 이현경, 김준호, 유순애, 안태석, 김치경, 이동훈. 2003. 독소생성 Microcystis 검출을 위한 PCR primer의 평가. 한국미생물학회지. 39:166-174.
18 정승현, 안치용, 최애란, 장감용, 오희목. 2005. 대청호에서 강우와 식물플랑크톤 군집의 관계. 환경생물. 23:57-63.
19 정 준. 1993. 한국담수조류도감. 도서출판 아카데미서적. 서울. 496pp.
20 천세억, 이재안, 이재정, 유영복, 방규철, 이열재. 2006. 대청호 유입유량 변동과 수질 및 조류증식의 관계. 수질보전 한국물환경학회지. 22:342-348.
21 Ahn CY, AS Chung and HM Oh. 2002. Rainfall, phycocyanin, and N : P rations related to cyanobacteria bloom in a Korean large reservoir. Hydrobiologia 474:117-124.   DOI
22 Choi HJ, BH Kim, JD Kim and MS Han. 2005. Streptomyces neyagawaensis as a control for the hazardous biomass of Microcystis aeruginosa (Cyanobacteria) in entrophic freshwaters. Biol. Control 33:335-343.   DOI   ScienceOn
23 Ahn CY, YS Park and HM Oh. 2011. Evaluation of environmental factors on cyanobacterial bloom in eutrophic reservoir using Artificial Neural Networks. J. Phycol. 47:495-504.   DOI   ScienceOn
24 Amann RI, W Ludwig and KH Schleiffer. 1995. Phylogenetic identification and in situ detection of individual microbial cells without cultivation. Microbiol. Rev. 59:143-169.
25 An KG and JR Jones. 2000. Factors regulating bluegreen dominance in a reservoir directly influenced by the Asian monsoon. Hydrobiologia 432:37-48.   DOI   ScienceOn
26 Crumpton WG, TM Isenhart and PD Mitchell. 1992. Nitrate and organic N analyses with second derivative spectroscopy. Limnol. Oceanogr. 37:907-913.   DOI   ScienceOn
27 D'Elia CF, PA Steudler and N Corwin. 1977. Determination of total nitrogen in aqueous sample using persulfate digestion. Limnol. Oceanogr. 22:760-764.   DOI   ScienceOn
28 김 명, 전은형, 안태영. 2003. 삽교호의 세균다양성과 계통분류학적 분석. 한국미생물학회지. 39:272-276.
29 고소라, 박성주, 안치용, 최애란, 이정숙, 김희식, 윤병대, 오희목. 2004. DGGE를 이용한 대청호 수화발생시기의 세균군집 분석. 한국미생물학회지. 40:205-210.
30 김동주, 홍선희, 안태석. 1999a. 소양호에서 세균군집구조의 계절적.수직적 변화. 한국미생물학회지. 35:242-247.
31 김명운, 김민호, 조장천, 김상종. 1995. Cyanobacteria의 증식에 따른 대청호 생태계내의 생물군집 변화. 한국육수학회지. 28:1-9.
32 김범철, 김호섭, 박호동, 최광순, 박종근. 1999b. 국내 호수에서 발생한 남세균의 microcystin함량과 독성평가. 한국육수학회지. 32:288-294.
33 박정아, 허건영, 이정숙, 오윤정, 김보연, 민태익, 김치경, 안종석. 2003. 김치의 저온 발효 중 미생물 변화 양상. 한국미생물학회지. 39:45-50.
34 박종근. 2005. 대청호의 남조세균 수화 발달 특성. 환경생물. 23:304-314.
35 빙혜선, 이상욱, 황순진, 오인혜. 2002. 대청호에서 계절에 따른 세균군집 및 환경요인의 변화. 한국육수학회지. 35:71-78.
36 오희목, 김도한. 1995. 대청호의 남조류 수화발생에 대한 단기적 예측. 한국육수학회지. 28:127-135.