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Cell Survival and Expression of Superoxide Dismutase and Catalase Genes in Saccharomyces cerevisiae Treated with N-acetyl-L-cysteine and Ionizing Radiation  

Park, Ji-Young (Korea Atomic Energy Research Institute, Advanced Radiation Technology Institute)
Baek, Dong-Won (Division of Applied Life Sciences (World Class University Program), Gyeongsang National University)
Nili, Mohammad (Dawnesh Radiation Research Institute)
Kim, Jin-Kyu (Korea Atomic Energy Research Institute, Advanced Radiation Technology Institute)
Publication Information
Korean Journal of Environmental Biology / v.29, no.1, 2011 , pp. 61-67 More about this Journal
Abstract
N-acetyl-L-cysteine (NAC) having a thiol, a precursor for glutathione (GSH), is known as one of the antioxidants. NAC used as a radioprotector against ionizing radiation (IR)-induced injury and damage. The aim of this study was to evaluate the radioprotective effects of NAC against IR-induced cell damage in Saccharomyces cerevisiae and the antioxidative effect of NAC on transcriptional level of yeast antioxidant enzyme genes such as superoxide dismutase (SOD) and catalase. In the present study, yeast cells were pretreated with various concentrations of NAC and/or irradiated with various doses of gamma rays. The cell viability was measured by counting the cell forming unit (CFU). The quantitative real-time PCR was performed for analysis of gene expression of SOD and catalase. The viability of irradiated cells was not improved by pretreatment with NAC. Ionizing radiation with 100 Gy highly induced the gene expression of antioxidant enzymes. In the irradiated group with NAC pretreatment, the gene expression of SOD and catalase was gradually reduced with the increased concentrations of NAC. These results indicate that NAC can act as a useful antioxidant to scavenge reactive oxygen species in vivo, but does not protect cells against IR-induced cell death in S. cerevisiae.
Keywords
ionizing radiation; reactive oxygen species; SOD; catalase; N-acetyl-L-cysteine; defense system; gene expression;
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