Browse > Article

Responses in Hepatic Xenobiotic Metabolizing Enzymes and Sex Hormones of Yellowfin Goby Acanthogobius flavimanus in Nakdong Estuary  

Lee, Ji-Seon (Division of Marine Bioscience and Technology/EMBRC, Kangnung National University)
Jeong, Jee-Hyun (KORDI South Sea Institute)
Han, Chang-Hee (Division of Life Science, Dong-eui University)
Shim, Won-Joon (KORDI South Sea Institute)
Jeon, Joong-Kyun (Division of Marine Bioscience and Technology/EMBRC, Kangnung National University)
Publication Information
Korean Journal of Environmental Biology / v.26, no.2, 2008 , pp. 87-93 More about this Journal
Abstract
To assess effects of contaminants on fish in Nakdong river, feral yellowfin goby Acanthogobius flavimanus were caugt in two different sites and its hepatic monooxygenase enzyme, including cytochrome P450 (CYP), NADPH-cytochrome P450 reductase (P450R), NADH-cytochrome b5 reductase (b5R), ethoxyresorufin deethylase (EROD), glutathione S-transferase (GST) were quantitatively determined. Gonadosomatic index (GSI), hepatosomatic index (HSI) and three sex steroid hormone (17$\beta$-estradiol, E2; testosterone, T; 11-ketotestosterone, 11-KT) levels of the fish were also investigated. HSI of fish from polluted site (site 1) were significantly higher than that of unpolluted site (site 2), but GSI levels were significantly lower in polluted site. No significant differences in plasma 11-KT and T levels were observed in two sites surveyed. E2 level was, however, significantly (p<0.05) higher in female fish from site 1 than site 2. In addition, hepatic EROD activity and CYP level of site 1 fish were lower than those of site 2 fish, whereas relatively high levels of P450R, b5R and GST activities were found in site 1. The results imply that yellowfin goby, especially female fish in Nakdong river estuary are affected from contaminants disrupting sex steroid hormone system.
Keywords
xenobiotic metabolizing enzyme; Acanthogobius flavimanus; HSI; GSI; 17$\beta$-estradiol; testosterone; 11-ketotestosterone; Nakdong-river;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 곽석남, 허성회. 2003. 낙동강 하구역 어류의 종조성 변화. 한국수산학회지. 36:129-135
2 김기현, 조진형, 박남준. 2000. 낙동강 하구역 퇴적물 중금속 의 분포와 오염의 역사 추정. 한국해양학회지. 5:285-294
3 문지용, 이성인, 송희영, 이경진, 최경희, 정기호. 2004. 낙동강 유역에 서식하는 붕어와 황소개구리 체내의 Polychlorinated biphenyls의 축적도와 분포특성. 분석과학. 17:347- 354
4 박명희, 황인준, 김대중, 이영돈, 김형배, 백혜자. 2005. 문절망 둑(Acanthogobius flavimanus)의 생식소 발달과 혈중 성 스테로이드호르몬 농도 변화. 한국수산학회지. 38:309- 315   과학기술학회마을   DOI
5 이지선, 정지현, 한창희, 심원준, 전중균. 2008. 시화호에서 채 집한 풀망둑 hasta의 간장 약물대사효소계 및 항산화계 의 반응. 환경생물. 26:94-101
6 정기호, 김문순, 정종학. 1996. 낙동강 하류 유역의 저니토, 토양, 잡초 및 채소 중의 중금속의 분포와 상관관계. 한국환경과학회지. 5:801-812
7 Goksoyr A, J Beyer, AM Husoy, HE Larsen, K Westrheim, S Wilhelmesen and J Klungsoyr. 1994. Accumulation and effects of aromatic and chlorinated hydrocarbons in juvenile Atlantic cod (Gadus morhua) and rainbow trout (Salmo gairdneri) Toxicol. Appl. Pharmacol. 89:347-360   DOI   ScienceOn
8 Kime DE. 1998. Endocrine Disruption in Fish. Kluwer Academic Publishers, Boston
9 Masfaraud JF, A Devaux, A Pffohl-Leszkowicz, C Malaveille and G Monod. 1992. DNA adduct formation and 7- ethoxyresorufin O-deethylase induction in primary culture of rainbow trout hepatocytes exposed benzo[a]pyrene. Toxic. in Vitro 6:523-531   DOI   ScienceOn
10 Omura T and S Takesue. 1970. A new method for simultaneous purification of cytochrome b5 and NADPH-cytochrome c reductase from rat liver microsomes. J. Biochem. 67:249-257   DOI
11 Philips AH and RG Langdon. 1962. Hepatic triphosphopyridine nucleotide-cytochrome c reductase: isolation, characterization, and kinetic studies. J. Biol. Chem. 237:2652-2660
12 Thomas P. 1988. Reproductive endocrine function in female atlantic croaker exposed to pollutants. Mar. Environ. Res. 24:179-183   DOI   ScienceOn
13 Thomas P. 1990. Teleost model for studying the effects of chemicals on female reproductive endocrine function. J. Exp. Zool. Suppl.4:126-128
14 서정관, 이재정, 양상용, 정익교. 2003. 낙동강 수계 하,폐수 처리시설의 방류수가 조류 성장 잠재력에 미치는 영향. 한국조류학회. 18:157-167
15 윤명희, 김지혜, 大津􃢄一, 민병윤. 2001. 낙동강 하구수의 변이원성에 대한 연구. 한국환경과학회지. 10:35-39
16 이순화, 임혜승, 김지훈, 김재경. 2000. 강둑여과수와 낙동강 수질의 변이원성에 의한 평가. 대한환경공학회지. 22:1671 -1681
17 ven der Oost A, A Goksoyr, M Celander, H Heida and NPE Vermeulen. 1996. Biomonitoring of aquatic pollution with feral eel (Anguilla angullid) II. Biomarkers: pollution-induced biochemical responses. Aquat. Toxicol. 36:189-222   DOI   ScienceOn
18 Ram RJ and AG Sathyanesan. 1983. Effect of mercuric chloride on the reproductive cycle of the teleostean fish Channa punctatus. Bull. Environ. Contam. Toxicol. 30:24-27   DOI
19 박영규, 이철희, 이순화, 김종우. 1996. 낙동강유역 하천수의 농약 유출 특성 : 농경지 및 골프장에서의 농약 유출의 평가. 대한환경공학회지. 18:627-636
20 Habig WH, MJ Pabst and WB Jakoby. 1974. Glutatione-Stransferase, the first enzymatic step in mercapturic acid formation. J. Biol. Chem. 249:7130-7139
21 Karels AE, R Soimasuo, J Lappivaara, H Leppannen, R Santiti and A Oikari. 1997. Effects of ECF bleached kraft mill effuent on reproductive steroids and liver MFO activity in perch and roach. pp. 257. In Proceedings of SETAC-Europe 7th Annual Meeting. Amsterdam
22 Livingstone DR. 1991 Organic xenobiotic metabolism in marine invertebrates. pp. 45-185. In Advances in Comparative and Environmental Physiology (Gilles R ed.). Springer, Berlin
23 김성철, 박청길, 조현서, 이대인. 2003. 낙동강 퇴적물의 Nonylphenol과 Bisphenol A 오염도 평가. 한국물환경학회지. 19:357-366
24 양성렬, 송환석, 문창호, 권기영, 양한섭. 2001b. 낙동강 하구 역의 담수유입에 따른 해양환경 및 일차생산력 변화. Algae. 16:165-177
25 양홍준, 김구환, 금지돈. 2001a. 낙동강하구의 어류상과 댐의 어도에서 어류의 이동. 육수지. 34:251-258
26 Aida K. 1988. A review of plasma hormone changes during ovulation in cyrinid fishes. Aquacult. 74:11-21   DOI   ScienceOn
27 Livingstone DR. 1998. The fate of organic xenobiotics in aquatic ecosystems: quantitative and qualitative differences in biotransformation by invertebrates and fish. Comp. Biochem. Physiol. 120A:43-49
28 Dey S and S Bhattacharya. 1989. Ovarian damage to Channa punctatus after chronic exposure to low concentrations of elsan, mercury, and ammonia. Ecotoxicol. Environ. Saf. 17:247-257   DOI   ScienceOn
29 Shukla JP and K Pandey. 1985. Ovarian recrudescence in a freshawter teleost, Saratherodon mossambicus in DDT treatment and pattern of recoupment. J. Environ. Biol. 6:195-204
30 Adams SM, LR Shugart, GR Southworth and DE Hinton. 1990. Application of biomarker of bioindicators in assessing the health of fish populations experiencing contaminant stress. pp. 333-353. In Biomarkers of Environmental Contamination (McCarthy JF and LR Shugart eds.). Lewis Publishers. Boca Raton
31 Burke MD and RT Mayer. 1974. Ethoxyresorufin: direct fluorometric assay of a microsomal O-dealkylation which is preferentially inducible by 3-methylcholanthrene. Drug Metab. Disp. 2:583-588
32 Pawar KR and M Katdare. 1983. Effect of sumithion on the ovaries of freshwater fish Garra mullya (Sykes). Curr. Sci. 16:784-785
33 Schlezinger JJ, WDJ Struntz, JV Goldstone and JJ Stegeman. 2006. Uncoupling of cytochrome P450 1A and stimulation of reactive oxygen species production by co-planar polychlorinated biphenyl congeners. Aquat. Toxicol. 77:422-432   DOI   ScienceOn
34 Omura T and R Sato. 1964. The carbon monoxide-binding pigment of liver microsomes. J. Biol. Chem. 239:2370-2378
35 문성기, 정종문, 최철만. 2001. 낙동강 중, 하류의 식물플랑크 톤 군집구조. 한국환경과학회지. 10:41-45
36 Lowry OH, NJ Roseborough, LA Farr and RJ Randall. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193:265-275