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Molecular Systematic Study of Bacterial Community Associated with Sand Dune Plants  

Do, Jin-Ok (Department of Microbiology and Biotechnology, Daejeon University)
Park, Seong-Joo (Department of Microbiology and Biotechnology, Daejeon University)
Kim, Seung-Bum (Department of Microbiology, Chungnam National University)
Publication Information
Korean Journal of Environmental Biology / v.25, no.4, 2007 , pp. 356-362 More about this Journal
Abstract
The rhizobacterial diversity associated with 9 native plant species inhabiting coastal sand dunes in Tae-an area, Chungnam Province, was studied using the denaturing gradient gel electrophoresis (DGGE) fingerprinting analysis over three times from October 2003 to March 2004. One dominant band commonly occurred in all of the rhizosphere samples, which was identified as that of Lysobacter enzymogenes. The other common bands included those derived from species of Pseudomonas and Bacillus. It was notable that L. enzymogenes was dominant in all of the 9 plant species and such dominance was consistent throughout the whole sampling period, which confirms the previous study by Lee et al. (2006a). The Bacillus bands were detected in all of the three samplings, and those of Pseudomonas were notable in the samples of December 2003. By the DGGE analysis alone, the significance of Lysobacter to the sand dune plants is not clear. However, considering their presence in healthy plants and the dominance in all plant species, Lysobacter may have positive roles in the survival or growth of the plants in sand dune area.
Keywords
rhizobacteria; sand dune; denaturing gradient gel electrophoresis; Lysobacter;
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Times Cited By KSCI : 3  (Citation Analysis)
연도 인용수 순위
1 안영희. 2003. 신두리 해안 사구지 식생의 식물사회학적 연구. J. Kor. Env. Res. Reveg. Tech. 6:29-40
2 Hagler AN, CA Rosa, PB Morais, LC Mendonca-Hagler, GM Franco, FV Araujo and CA Soares. 1993. Yeasts and coliform bacteria of water accumulated in bromeli-ads of mangrove and sand dune ecosystems of southeast Brazil. Can. J. 39: 973-977   DOI   ScienceOn
3 Matekwor Ahulu E, M Nakata and M Nonaka. 2005. Arumand Paris-type arbuscular mycorrhizas in a mixed pine forest on sand dune soil in Niigata Pre-fecture, central Honshu, Japan. Mycorrhiza 15:129-136   DOI
4 Park MS, SR Jung, KH Lee, MS Lee, JO Do, SB Kim and KS Bae. 2005. Isolation and characterization of bacterial associated with two sand dune plants species, Calystegia soldanella and Elymus mollis. J. Microbiol. 43:219-227   과학기술학회마을
5 Weon HY, BY Kim, YK Baek, SH Yoo, SW Kwon, E Stackebrandt and SJ Go. 2006. Two novel species, Lysobacter daejeonensis sp. nov. and Lysobacter yangpyeongensis sp. nov., isolated from Korean greenhouse soils. Int. J. Syst. Evol. Microbiol. 56:947-951   DOI   ScienceOn
6 Huguet V, JM Batzli, JF Zimpfer, P Normand, JO Dawson and MP Fernandez. 2001. Diversity and specificity of Frankia strains in nodules of sympatric Myrica gale, Alnus incana and Shepherdia canadensis determined by rrs gene polymorphism. Appl. Environ. Microbiol. 67:2116-2122   DOI   ScienceOn
7 정용규, 김종원. 1998. 한국의 해안사구 식생. Kor. J. Ecol. 21:257-262
8 Morais PB, CA Rosa, AN Hagler and LC Mendonca-Hagler. 1994. Yeast communities of the cactus Pilo-socereus arrabidae as resources for larval and adult stages of Drosophila serido. Antonie Leeuwenhoek 66:313-317   DOI
9 이우철, 전상근. 1983. 한국 해안식물의 생태학적 연구-남해안의 사구식물 군락의 종조성과 현존량. Kor. J. Ecol. 6:177-186
10 Lee MS, JO Do, MS Park, S Jung, KH Lee, KS Bae, SJ Park and SB Kim. 2006a. Dominance of Lysobacter sp. in the rhizosphere of two coastal sand dune plant species, Calystegia soldanella and Elymus mollis. Antonie Leeuwenhoek 90:19-27   DOI   ScienceOn
11 Shin DS, MS Park, SR Jung, MS Lee, KH Lee, KS Bae and SB Kim. 2007. Plant growth-promoting potential of endophytic bacteria isolated from roots of coastal sand dune plants. J. Microbiol. Biotechnol. 17:1361-1368   과학기술학회마을
12 Christensen P and FD Cook. 1978. Lysobacter, a new genus of nonfruiting, gliding bacteria with a high base ratio. Int. J. Syst. Bacteriol. 28:367-393   DOI
13 Lee JW, WT Im, MK Kim and DC Yang. 2006b. Lysobacter koreensis sp. nov., isolated from a ginseng field. Int. J. Syst. Evol. Microbiol. 56:231-235   DOI   ScienceOn
14 Mudryk ZJ. 2005. Occurrence and distribution antibiotic resistance of heterotrophic bacteria isolated from a marine beach. Mar. Pollut. Bull. 50:80-86   DOI   ScienceOn
15 김준태, 리기현, 정병철, 김종균. 1993. 사구식물과 Arbuscular 내생 균근균의 공생 특성. Kor. J. Mycol. 21:235-245
16 변무섭, 박준모. 2002. 우이도 사구의 식물상 및 비오톱 보전에 관한 연구. 한국산림휴양학회지 6:93-101
17 Epstein DM and PC Wensink. 1988. The alpha-lytic protease gene of Lysobacter enzymogenes. The nucleo-tide sequence predicts a large prepro-peptide with homology to pro-peptides of other chymotrypsin-like enzymes. J. Biol. Chem. 263:16586-16590
18 Maremmani A, S Bedini, I Matosevic, PE Tomei and M Giovannetti. 2003. Type of mycorrhizal associations in two coastal nature researves of the editerranean basin. Mycorrhiza 13:33-40   DOI   ScienceOn
19 고성덕, 박주영. 2003. 충남 보령군 신두리 사구의 arbuscular mycorrhizal fungi (AMF)의 종조성과 분포에 관한 연구. Bullet. Sci. Edu. 19:189-203
20 이우철, 전상근. 1984. 한국 해안식물의 생태학적 연구-서해안의 사구식생에 관하여. Kor. J. Ecol. 7:74-84
21 Kowalchuk GA, JR Stephen, W De Boer, JI Prosser, TM Emb-ley and JW Woldendorp. 1997. Analysis of ammonia-oxidizing bacteria of the beta subdivision of the class Proteobacteriain coastal sand dunes by dena-turing gradient gel electrophoresis and sequencing of PCR-amplified 16S ribosomal DNA fragments. Appl. Environ. Microbiol. 63:1489-1497
22 Kowalchuk GA, FA deSouza and JA van Veen. 2002. Community analysis of arbuscular mycorrhizal fungi associated with Ammophila arenaria in Dutch coastal sand dune. Mol. Ecol. 11:571-581   DOI   ScienceOn
23 Bae HS, WT Im and ST Lee. 2005. Lysobacter concretionis sp. nov., isolated from anaerobic granules in an upflow anaerobic sludge blanket reactor. Int. J. Syst. Evol. Microbiol. 55:1155-1161   DOI   ScienceOn
24 Im BS, JS Lee and HS Kim. 2003. Changes of the coastal sand dune vegetation after the construction of embank-ment in Anmado. Kor. J. Ecol. 26:103-108   과학기술학회마을   DOI
25 Clough KS and JC Sutton. 1978. Direct observation of fungal aggregates in sand dune soil. Can. J. Microbiol. 24:333-335   DOI   ScienceOn
26 Rosa CA, MA Lachance, WT Starmer, JS Barker, JM Bowles and B Schlag-Edler. 1999. Kodamaea nitidulidarum, Candida restingae and Kodamaea anthophila, three new related yeast species from ephemeral flowers. Int. J. Syst. Bacteriol. 49:309-318   DOI   ScienceOn