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Enhancement of Pendimethalin Degradation Activity in Bacillus sp. MS202 using Gamma Radiation  

Lee Young-Keun (Radiation Application Research Division, ARTIJ, Korea Atomic Energy Research Institute)
Chang Hwa-Hyoung (Radiation Application Research Division, ARTIJ, Korea Atomic Energy Research Institute)
Lee Ho-Jin (Radiation Application Research Division, ARTIJ, Korea Atomic Energy Research Institute)
Park Heesoon (Radiation Application Research Division, ARTIJ, Korea Atomic Energy Research Institute)
Lee Kyung Hee (Radiation Application Research Division, ARTIJ, Korea Atomic Energy Research Institute)
Joe Min-Ho (Radiation Application Research Division, ARTIJ, Korea Atomic Energy Research Institute)
Publication Information
Korean Journal of Environmental Biology / v.23, no.4, 2005 , pp. 405-408 More about this Journal
Abstract
To induce the enhanced mutants of dinitroaniline herbicide pendimethalin degrading bacterium, Bacillus sp. MS202 was irradiated with gamma radiation at the dose of $LD_{99}$ (3.35 kGy). Three enhanced mutants (MS202m7, MS202m14, MS202m18) were isolated from the candidates by the generation - isolation method. Clear zone formation and the GC analysis confirmed that the degrading activity of each enhanced mutant (MS202m7, MS202m14, MS202m18), the formation of pendimethalin metabolite, increased by $11\%,\;45\%,\;and\;32\%$ than a wild type, respectively. It suggested that these mutants induced by gamma radiation could be useful for the application of pesticide degradation.
Keywords
pendimethalin degradation; Bacillus sp.; gamma radiation; enhanced mutant;
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Times Cited By KSCI : 2  (Citation Analysis)
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1 Chen Wand A Mulchandani. 1998. The use of live biocatalysts for pesticide detoxification. Trends Biotechnol. 16:71-76   DOI   ScienceOn
2 Kole RK, J Saha, S Pal, S Chaudhuri and A Chowdhury. 1994. Bacterial degradation of the herbicide pendimetha1in and activity evaluation of its metabolites. Bull. Environ. Contam. Toxicol. 52:779-786
3 Tubiana M, J Dutreix and A Wambersie. 1990. Introduction to radiobiology. Taylor and Francis, New York, pp. 1-33
4 Engelhardt G, PR Wallnofer and R Plapp. 1973. Purification and properties of an aryl acylamidase of Bacillus sphaericus, catalyzing the hydrolysis of various phenyl amide herbicides and fungicides. Appl. Microbiol. 26:709-710
5 Lee Y-K, H-H Chang, Y-S Jang and J-H Huh. 2004. Microarray analysis of radiation related gene expression in mutants of Bacillus lentimorbus WJ5 induced by gamma radiation. Kor. J. Environ. Biol. 22:472-477
6 Friedberg EC. 2003. DNA damage and repair. Nature 421:436-440   DOI   PUBMED   ScienceOn
7 Lee Y-K, J-S Kim, H-Y Chung, Y-S Jang and B-I Jang. 2003. Two-dimensional electrophoresis analysis of proteins between Bacillus licheniformis DM3 and its antifungal activity deficient mutant. Kor. J. Envir. Agri. 22: 203-209   DOI
8 Tomlin CDS. 2003. The pesticide Manual, 13th (ed), British Crop Protection Council, Bracknell, UK. pp. 752-753
9 Lee Y-K, H-H Chang, Y-S Jang, S-W Hyung and H-Y Chung. 2004. Partial reduction of dinitroaniline herbicide pendimethalin by Bacillus sp. MS202. Kor. J. Environ. Agr. 23:197-202   DOI
10 US EPA. 1999. Persistent bioaccumulative toxic (PBT) chemicals. Final Rule., Fed. Regist. 64:58666-58753
11 Gita K, BS Shashi, PL Shashi and TY Nanjapur. 2000. Effect of long-term application of pendimethalin: enhanced degradation in soil. Pest Manag. Sci. 56:202-206   DOI   ScienceOn
12 Singh SB and G Kulshrestha. 1991. Microbial degradation of pendimethalin. J. Environ. Sci. Health B26:309-321