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Expression Changes of Chironomus riparius Proteome reflecting Ecdysterone Inducible Genes and Mitogenome exposure to Bisphenol-A  

Kwak Inn-Sil (Department of Life Science, College of Natural Science, Hanyang University)
Publication Information
Korean Journal of Environmental Biology / v.23, no.3, 2005 , pp. 275-280 More about this Journal
Abstract
Development of the fourth-instar larvae of Chironomus riparius has a sensitive to ecdysteroid hormones. The 2D/E gel analysis for polypeptide expression reflecting early-ecdysterone inducible gene has conducted the emerged female from larval phase exposure to bisphenol A (BPA). In the 2D/E gel 1108 protein spots were identified. The visualized protein spots allowed extraction of 17 protein spots differed more than 3 fold in BPA treated animals, which was approximately $1.6\%$ of the total protein spots. However, polypeptide expression reflecting early-ecdysterone inducible gene didn't change after treatments. In addition, detection for the damages or changes in mitogenome level was observed. The conserved cytochrome oxidase I in DNA level affected exposure to BPA $(1{\mu}gL^{-1})$ in this preliminary study.
Keywords
Chironomus riparius; cytochrome oxidase I; polypeptide expression; bisphenol A;
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