Browse > Article

Genetic Engineering for Detection of Endocrine Disruption using I-18 C Gene Expression in Chironomus riparius  

Kwak Inn-Sil (Department of Life Science, College of Natural Science, Hanyang University)
Publication Information
Korean Journal of Environmental Biology / v.23, no.3, 2005 , pp. 269-274 More about this Journal
Abstract
The 2D/E gel analysis for polypeptide expression reflecting I-18 C gene (early-ecdysterone inducible gene) has conducted the emerged C. riparius adults from larval phase exposure to tebufenozide acting as an ecdysteroidal molting hormone. Control group, the amount of ORE II of the I-18 C gene was larger than that of ORE I of this gene. After treatments, ORE I of the I-18 C gene was overexpressed as the polypeptide, whereas ORF II of this gene was expressed as the polypeptide and was clearly reduced expression. Accordingly, we consider that tebufenozide exhibited endocrine disruptions related processing of ecdysteroid receptor protein reflecting ORF II of I-18 C gene. Also, earlier emergence day was related overexpressed polypeptide reflecting ORE I of I-18 C gene. In this study result, tebufenozide induced changing of physiological condition, and then polypeptide expression reflecting early-ecdysterone inducible I-18 C gene was different between control group and exposure group.
Keywords
Chironomus riparius; I-l8C gene; polypeptide expression; tebufenozide;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Borowicz BP. 2005. Isolation, cloning and expression of DNA fragments reflecting open reading frames of 1.8 and 4.6 kb RNAs of the I-18 C gene of Chironomus tentans. J. Appl. Genet. 46:75-83
2 Elendt BP and WR Bias. 1990. Trace nutrient deficiency in Daphnia magna cultured in standard medium for toxicity testing; effects of the optimization of culture conditions on life history parameters of Daphnia magna. Water. Res. 24:1157-1167   DOI   ScienceOn
3 Elke C, P Rauch, M Spindler-Barth and K-D Spindler. 1999. DNA-binding properties of the ecdysteroid receptor-complex (EcR/USP) of the epithelial cell line from Chironomus tentans. Arch. Insect. Biochem. Physiol. 41:124-133   DOI   ScienceOn
4 Helfman DM, JR Feramisco, JC Fiddes, GP Thomas and SH Hughes. 1983. Identification of clones that encode chicken tropomyosin by direct immunological screening of a cDNA expression library. Proc. Natl. Acad. Sci. USA. 80:31-35
5 Messing J and J Vieira. 1982. A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments. Gene 19:269-276   DOI   ScienceOn
6 Oakley BR, DR Kirsch and NR Morris. 1980. A simplified ultrasensitive silver stain for detecting proteins in polyacrylamide gels. Anal. Biochem. 105:361-363   DOI   ScienceOn
7 Streloke M and H Kopp. 1995. Long-term toxicity test with Chironomus riparius: development and validation of a new test system. Blackwell, Beriin/Vienna
8 Mouillet J-F, VC Henrich, M Lezzi and M Vogtli. 2001. Differential control of gene activity by isoforms A, B1 and B2 of the Drosophila ecdysone receptor. Eur. J. Biochem. 268:1811-1819   DOI   ScienceOn
9 Vogtli M, MO Imhof, NE Brown, P Rauch, M Spindler-Barth, M Lezzi and VC Henrich. 1999. Functional characterization of two ultraspiracle forms (CtUSP-l and CtUSP-2) from Chironomus tentans. Insect Biochem. Mol. Biol. 29: 931-942   DOI   ScienceOn
10 Dorsch-Hasler K, B Lutz, K-D Spindler and M Lezzi. 1990. Structural and developmental analysis of a gene cloned from the early ecdysterone-inducible puff site, I-18 C, in Chironomus tentans. Gene 96:233-239   DOI   ScienceOn
11 Bradford MM. 1976. A rapid and sensitive method for microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248-54   DOI   PUBMED   ScienceOn
12 Kwak I.-S. and W Lee. 2005. Two-demensional gel electrophoresis analysis of proteins following tebufenozide treatment of Chironomus riparius. Bull. Environ. Contam. Toxicol. 74:1159-1165   DOI   ScienceOn
13 Clare AS, D Rittschof and JD Costlow. 1992. Effects of nonsteroidal ecdysone mimic RH 5849 on larval crustaceans. J. Exp. Zool. 262:436-440   DOI
14 Hertner T, B Hertner-Meyer, JM Sogo, G Schlapfer, H Jackle, JE Edstrom and M Lezzi. 1986. Microcloning of a long segment of a gene from the primary ecdysterone-controlled puff region I-18 C of Chironomus tentans: molecular characterization and effect of hormone on its transcription. Develop. Biol. 113:29-39   DOI
15 Lezzi M, B Lutz and K Dorsch-Haesler. 1989. The ecdysterone-controlled chromosome region I-18 C in C. tentans: from cytology to molecular biology. Acta. Biol. Debr. Oecol. Hung. 2:129-139
16 Kwak I.-S. and W Lee. 2004. Effects of Ecdysteroid agonist Tebufenozide on freshwater Chironomids. Korean J. Limnol. 37:96-101
17 Retnakaran A, K Hiruma, SR Palli and LM v. 1995. Molecular analysis of the mode of action of RH-5992, a lepidopteran-specific, non-steroid agonist. Insect Hem. Mol. Bio1. 25: 109-117
18 Turberg A and K-D Spindler. 1992. Properties of nuclear and cytosolic ecdysteroid receptors from an epithelial cell line from Chironomus tentans. J. Insect Physiol. 38:81-91   DOI   ScienceOn
19 Vogtli M, C Elke, M Imhof and M Lezzi. 1998. High level transactivation by the ecdysone receptor complex at the core recognition motif. Nucleic Acids Res. 26:2407-2414   DOI   PUBMED
20 Dhadialla TS, GS Carlson and P LeDat. 1998. New insecticides with ecdysteroidal and juvenile hormone activity. Annu. Rev. Entomol. 43:545-569   DOI   ScienceOn
21 Wegmann IS, S Quack, K-D Spindler, K Dorsch-Hasler, M Vogtli and M Lezzi. 1995. Immunological studies on the developmental and chromosomal distribution of ecdysteroid receptor protein in Chironomus tentans. Arch. Insect Biochem. Physiol. 30:95-114   DOI   ScienceOn
22 Amrein K, K Dorsch-Hasler, B Lutz and M Lezzi. 1988. Two transcripts of the same ecdysterone-controlled gene are differentially associated with ribosomes. Gene 65:277-283   DOI   ScienceOn
23 Kozak M, 1986. Bifunctional Messenger RNAs in Eukaryotes. Cell 47:481-483   DOI   PUBMED   ScienceOn
24 Lezzi M, T Bergman, VC Henrich, M Vogtli, C Fromel, M Grebe, S Przibilla and M Spindler-Barth. 2002. Ligandinduced heterodimerization between the ligand binding domains of the Drosophila ecdysteroid receptor and ultraspiracle. Em. J. Biochem. 269:3237-3245   DOI   ScienceOn
25 Hahn T, L Matthias and S Ralf. 2001. Effects of the hormone mimetid insecticide tebufenozide on Chironomus riparius larvae in two different exposure setups. Ecotox. Environ. Saf. 49:171-178   DOI   ScienceOn
26 Lezzi M, F Gatzka, H Ineichen and AD Gruzdev. 1991. Transcriptional activation of puff site I-18 C of Chironomus tentans: Hormonal responsiveness changes in parallel with diurnal decondensation cycle. Chromosoma 100:235-241   DOI
27 Wing KD. 1988. RH-5849, a nonsteroidal ecdysone agonist: Effects on a Drosophila cell line. Science 241:467-469   DOI   PUBMED
28 Amrein K, B Lutz, T Hertner, M Lezzi and K Dorsch-Haesler. 1986. Complete DNA sequence of the ecdysterone-controlled gene I-18 C of Chironomus tentans. Nucl. Acids. Res. 14:7807-7808   DOI   ScienceOn
29 USEPA. 1994. Methods for measuring the toxicity and bioaccumulation of sediment-associated contaminants with freshwater invertebrates, United States Environmental Protection Agency EPA/600/R-94/024, Technical report, Washington DC 133pp