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In vitro Response of Cytochrome P450 (CYP) and EROD Activity in Bivalve (Coelomactra antiquata) by DDT, PCB-153 and TCDD  

전중균 (강릉대학교 해양생명공학부)
이미희 (강릉대학교 해양생명공학부)
이지선 (강릉대학교 해양생명공학부)
이경선 (일본어 장기대학)
심원준 (한국해양연구원)
신영범 (한국해양연구원)
이수영 (한국해양연구원)
Publication Information
Korean Journal of Environmental Biology / v.21, no.2, 2003 , pp. 170-176 More about this Journal
Abstract
The effect of xenobiotics on cytochrome P450 (CYP) and 7-ethoxyresorufin-O-deethylase (EROD) in hepatopancreatic microsome of surf clam, Coelomactra anti-quata, were investigated. The microsome isolated from the digestive gland of the surf clam, collected from the east coast of Korea, was in vitro exposed to p, p -DDT (0.1,0.4 and 1.0 mM) for 30 min and 2,3,7,8-TCDD (0.01, 0.04 and 0.1 ppb) and PCB-153 (0.01, 0.04 and 0.1 ppb) for 7 hr. In the case of DDT exposure, the CYP content and EROD activity of 1.0 mM exposure group increased up to about 117% and 120% of the DMSO solvent control group after 10 min. exposure, respectively. After 2 hr exposure of TCDD, the CYP content and EROD activity were also induced to the range of 103∼110% and 121∼139%, respectively. The PCB-153 exposure group showed 107∼115% of CYP content and 129∼140% of EROD activity after 2 hr exposure. Three test chemicals apparently induced CYP and EROD activity in the microsome of surf clam. The inducing potentials depend en the test chemicals.
Keywords
clam; Coelo antiquata; cytrochrome P450; EROD; DDT; TCDD; PCB-153;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
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