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In vitro Induction of Hepatic Cytochrome P450 (CYP) with Exposure to $\beta$-naphthoflavone in Marine Fishes  

전중균 (강릉대학교 해양생명공학부·동해안해양생물자원연구센터 (EMBRC))
이미희 (강릉대학교 해양생명공학부·동해안해양생물자원연구센터 (EMBRC))
이지선 (강릉대학교 해양생명공학부·동해안해양생물자원연구센터 (EMBRC))
심원준 (한국해양연구원 해양환경기후연구본부)
이수형 (한국해양연구원 해양환경기후연구본부)
허형택 (한국해양연구원 해양생물자원연구센터 서울해양과학연구소 (SIOS))
Publication Information
Korean Journal of Environmental Biology / v.21, no.1, 2003 , pp. 26-30 More about this Journal
Abstract
Cytochrome P450 (CYP) induction was determined in microsomes of three aquacultured fish species (Sebastes schlegeli, Paralichthys olivaceus and Pagrus major) and two wild fish species (Mugil cephalus and Stephanolepis cirrhifey) in vitro exposed to $\beta$-naphthoflavone (BNF). The microsomes of five fish were exposed to BNF (5 mM or 10 mM) in dimethylsulfoxide at $30^{\circ}C$ for 9 hr. The CYP contents in most fish increased according to exposure duration for 3 or 5 hour, and then decreased, while steady increase of CYP was observed in P. major for 9 hour. The induction of CYP contents in aquacultured fish species (207~422%) were higher than those in wild fish species (206~207%).
Keywords
$\beta$-naphthoflavone; cytochrome P450; fish; in vitro; olive flounder; rockfish; sea bream; mullet; filefish;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
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