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http://dx.doi.org/10.7847/jfp.2022.35.1.103

Effects of dietary by-products discarded after probiotics production (BPPP) on growth performance, innate immunity, immune gene expression, and disease resistance against Edwardsiella tarda in carp, Cyprinus carpio  

Choi, Jae Hyeok (Department of Aquatic Life Medicine, College of Ocean Science and Technology, Kunsan National University)
Jung, Sang Mok (Department of Aquatic Life Medicine, College of Ocean Science and Technology, Kunsan National University)
Yang, Eun Chong (Department of Aquatic Life Medicine, College of Ocean Science and Technology, Kunsan National University)
Jang, Tae Won (Department of Aquatic Life Medicine, College of Ocean Science and Technology, Kunsan National University)
Lee, Chan Heun (Geum Sung Sang Gong co., Ltd.)
Park, Kwan Ha (Department of Aquatic Life Medicine, College of Ocean Science and Technology, Kunsan National University)
Choi, Sanghoon (Department of Aquatic Life Medicine, College of Ocean Science and Technology, Kunsan National University)
Publication Information
Journal of fish pathology / v.35, no.1, 2022 , pp. 103-111 More about this Journal
Abstract
This study has been performed to investigate the potential effects of by-product discarded after probiotics production (BPPP) on growth performance, immune gene expression, innate-immunity status, and disease resistance of carp, Cyprinus carpio. For 3 weeks, carp were fed four diets containing different levels of BPPP at 0, 0.1, 0.2 and 0.5% per kg of normal diet. Every 7 days of feeding, immune-related gene expression, serum lysozyme activity and ACH50 were analyzed. Growth rates and challenge test with E. tarda were conducted after 3 weeks of BPPP feeding. Both lysozyme activity and ACH50 were significantly (p<0.05) increased in all BPPP supplemented groups compared to the control at every 7 day for 3 weeks of feeding trial. The gene expression of pro-inflammatory cytokines, IL-1β and TNF-α was significantly (p<0.05) up-regulated until 21 days of feeding in all groups except for 0.2% group on day 7 post feeding. The anti-inflammatory cytokine IL-10 gene expression was only significantly (p<0.05) increased in 0.1% group on day 7 and decreased (p<0.05) on day 14 in all BPPP supplemented groups. On day 21, the IL-10 gene expression was augmented (p<0.05) in all groups. SOD gene expression was significantly (p<0.05) increased compared to the control on day 14 and 21 post feeding, whereas no significant difference was observed on day 7. In challenging test, 0.2%, 0.1%, 0.5% and control group showed 80%, 70%, 60% and 40% of survival rate, respectively. Feed conversion rate was only improved in 0.5% group. In conclusion, the present study indicates that dietary BPPP suplementation improved growth performance, innate immune response and bactericidal activity in carp.
Keywords
Lactobacillus; Postbiotics; Bactericidal activity; Immune-related gene expression; Growth performance;
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1 Lopes, L. M. F., Mello, M. M. M. D. and Urbinati, E. C.: β-Glucan reduces cortisol plasma levels, enhances innate immune system after A. hydrophila inoculation, and has lipolytic effects on the pacu (Piaractus mesopotamicus). Aquaculture, 546, 2022.
2 Mohammadian, T., Monjezi, N., Peyhan, R. and Mohammadian, B.: Effects of dietary probiotic supplements on growth, digestive enzymes activity, intestinal histomorphology and innate immunity of common carp (Cyprinus carpio): a field study. Aquaculture, 549: 737787, 2022.   DOI
3 Marta, L. P., Maria, M. C., Antonio, F. and Beatriz, N.: β-glucan administration induces metabolic changes and differential survival rates after bacterial or viral infection in turbot (Scophthalmus maximus). Fish & Shellfish Immunol., 82: 173-182, 2018.   DOI
4 Song, J. Y., Sasaki, K., Okada, T., Sakashita, M., Kawakami, H., Matsuoka, S., Kang, H. S., Nakayama, K., Jung, S. J., Oh, M. J. and Kitamura, S. I.: Antigenic differences of the scuticociliate Miamiensis avidus from Japan. J. Fish Dis., 32: 1021-1034, 2009.
5 Vinay, T. N., Kim, Y. J., Jung, M. H., Kim, W. S., Kim, D. H. and Jung, S. J.: Inactivated vaccine against viral hemorrhagic septicemia (VHS) emulsified with squalene and aluminum hydroxide adjuvant provides long term protection in olive flounder Paralichthys olivaceus. Vaccine, 31: 4603-4610, 2013.   DOI
6 Seo, J. S., Jeon, E. J., Lee, E. H., Jung, S. H., Park, M. A., Jee, B. Y. abd Kim, N. Y.: The Residues of Enrofloxacin in Cultured Paralichthys olivaceus. J. Fish Pathol., 26(1): 45-50, 2013.   DOI
7 Ganguly, S., Dora, K. C., Sarkar, S. and Chowdhury, S.: Supplementation of prebiotics in fish feed: a review. Rev. Fish Biol. Fisheries, 23: 195-199, 2013.   DOI
8 Aguilar-Toala, J. E., Garcia-Varela, R., Garcia, H. S., Mata-Haro, V., Gonzalez-Cordova, A. F., Vallejo-Cordoba, B. and Hernandez-Mendoza, A.: Postbiotics: An evolving term within the functional foods field. Trends in Food Science & Technology, 75: 105-114, 2018.   DOI
9 Bang, S. J., Lee, C. H., Kang, T. Y., Choi, J. H., Jung, S. M., Kang, I. S., Park, K. H. and Choi, S. H.: Effects of Citrus unshiu Markovich on growth performance and bactericidal activity of nile tilapia Oreochromis niloticus. J. Fish Pathol., 32(2): 105-111, 2019.
10 Delzenne, N. M., Daubioul, C., Neyrinck, A., Lasa, M. and Taper, H. S.: Inulin and oligofructose modulate lipid metabolism in animals: review of biochemical events and future prospects. Brit. Nutr., 87: 255-259, 2002.   DOI
11 Maria Jose, H. G. and Elena, F. R.: Postbiotics in human health: Possible new functional ingredients?. Food Research International, 137, 2020.
12 Baek, M. S., Hwang, Y. S. and Choi, S. H.: The effects of a dietary Edwardsiella tarda specific bacteriophage and Bacillus subtilis mixture on innate immune responses and antibacterial activity of Nile tilapia Oreochromis niloticus. Kor. J. of Fisheries and Aquat. Sci., 26(3): 185-191, 2013.
13 Han, H. J., Kim, S. J., Kim, T. H., Kim, M. S., Cho, M. Y. and Choi, H. S.: Current Status and Future Directions of Fish Vaccines in Korea. Korean J. Fish Aquat. Sci., 54(4): 369-376, 2021.   DOI
14 Jorge, G. V., Toshiro, M. and Hidetsuyo, H.: Effect of Continuous and Interval Administration of Peptidoglycan on Innate Immune Response and Disease Resistance in Japanese Flounder Paralichthys olivaceus. Aquaculture Science, 54(2): 163-170, 2006.
15 KOSIS (Korean Statistical Information Service): https://kosis.kr/statHtml/statHtml.do?orgId=101&tblId=DT_1EW0001&vw_cd=MT_ZTITLE&list_id=K2_7&scrId=&seqNo=&lang_mode=ko&obj_var_id=&itm_id=&conn_path=MT_ZTITLE&path=%252FstatisticsList%252FstatisticsListIndex.do. 2022.
16 Kristina, B., Ine, E. P. and Raimo O. H.: The use of probiotics in fish feed for intensive aquaculture to promote healthy guts. Adv. Aquacult. Fish. Manage., 3(7): 264-273, 2015.
17 Nazhand, A., Souto, E. B., Lucarini, M., Souto, S. B., Durazzo, A. and Santini, A.: Ready to Use Therapeutical Beverages: Focus on Functional Beverages Containing Probiotics, Prebiotics and Synbiotics. Beverages, 6(2): 26, 2020.   DOI
18 Reyes-Cerpa, S., Maisey, K., Reyes-Lopez, F., Toro-Ascuy, D., Sandino, A. M. and Imarai, M.: Fish Cytokines and Immune Response. New Advances and Contributions to Fish Biology, Hakan Turker, IntechOpen, pp.3-58, 2012.
19 Tsilingiri, K. and Rescigno, M.: Postbiotics: What else?. Beneficial Microbes, 4(1): 101-107, 2013.   DOI
20 Piazzon, M. C., Calduch-Giner, J. A., Fouz, B., Estensoro, I., Simo-Mirabet, P., Puyalto, M., Karalazos, V., Palenzuela, O., Sitja-Bobadilla, A. and Perez-Sanchez, J.: Under control: how a dietary additive can restore the gut microbiome and proteomic profile, and improve disease resilience in a marine teleostean fish fed vegetable diets. Microbiome, 5(1): 164, 2017.   DOI
21 Wegh, C. A. M., Geerlings, S. Y., Knol, J., Roeselers, G. and Belzer, C.: Postbiotics and Their Potential Applications in Early Life Nutrition and Beyond. Int. J. Mol. Sci., 20(19): 4673, 2019.   DOI
22 Yano, T.: Assay of hemolytic complement activity. In: Stolen, J. S., Fletcher, T. C., Anderson, D. P., Hattari, S. C., Rowley, A. F., editors. Tech in fish immunol. Now Jersey: SOS Publications, pp. 131-141, 1992.
23 Tian, L., Zhou, X. Q., Jiang, W. D., Liu, Y., Wu, P., Jiang, J., Kuang, S. Y., Tang, L., Tang, W. N., Zhang, Y. A., Xie, F. and Feng, L.: Sodium butyrate improved intestinal immune function associated with NF-kB and p38MAPK signalling pathways in young grass carp (Ctenopharyngodon idella). Fish & Shellfish immunol., 66: 548-563, 2017.   DOI
24 Ramirez, N. C. B., Rodrigues, M. S., Guimaraes, A. M., Guertler, C., Rosa, J. R., Seiffert, W. Q., Andreatta, E. R. and do Nascimento Vieira, F.: Effect of dietary supplementation with butyrate and probiotic on the survival of Pacific white shrimp after challenge with Vibrio alginolyticus. Revista Brasileira de Zootecnia, 46(6): 471-477, 2017.   DOI
25 Klemashevish, C., Wu, C., Howsmon, D., Alaniz, R. C., Lee, K. and Jayaraman, A.: Rational identification of diet-derived postbiotics for improving intestinal microbiota function. Current Opinion in Biotechnology, 26: 85-90, 2014.   DOI
26 Ang, C. Y., Sano, M., Dan, S., Leelakriangsak, M. and Lal, T. M.: Postbiotics Applications as Infectious Disease Control Agent in Aquaculture. Biocontrol Science, 25(1): 1-7, 2020.   DOI
27 Cho, K. K., Lee, S. H., Choi, I. S. and Lee, S. W.: Nextgeneration Probiotics, Parabiotics, and Postbiotics. J. of Life Sci., 36(6): 595-602, 2021.
28 Gao, T., Xu, Y., Wang, K., Deng, Y., Yang, Y., Lu, Q., Pan, J. and Xu, Z.: Comparative LC-MS based nontargeted metabolite profiling of the Chinese mitten crab Eriocheir sinensis suffering from hepatopancreatic necrosis disease (HPND). Aquaculture, 491: 338-345, 2018.   DOI