[KSCI] Korea Science Citation Index Service

http://dx.doi.org/10.7847/jfp.2021.34.2.261

Comparison of sample preparation methods for quantification of febantel, an anthelmintic agent, and its metabolites in rockfish (Sebastes schlegeli) muscle using liquid chromatography-tandem mass spectrometry

Lim, Jae-Woong (Department of Aqualife Medicine, Chonnam National University)
Kwon, Inyeong (Smart Aquaculture Research Center, Chonnam National University)
Kim, Taeho (Department of Marine Production Management, Chonnam National University)
Kim, Wi-Sik (Department of Aqualife Medicine, Chonnam National University)
Kang, So Young (Department of Aqualife Medicine, Chonnam National University)

Publication Information

Journal of fish pathology / v.34, no.2, 2021 , pp. 261-269 More about this Journal

Abstract

This study presents the evaluation of sample extraction and purification procedure for the determination of residues of febantel and its metabolites, fenbendazole, oxfendazole and oxfendazole sulfone in rockfish (Sebastes schlegeli) muscle using liquid chromatography-tandem mass spectrometry. Residues of febantel and its metabolites in rockfish muscle were analyzed using each different sample preparation method from Korean Food Standards Codex (KFSC), Food Safety and Inspection Service (FSIS, USA), and the modified FSIS method using QuEChERS kit (FSIS-Q), respectively. Each method was compared for mean recoveries and repeatabilities. Since FSIS-Q showed higher repeatabilities (coefficient of variation, CV of 2.4%~10.9%) than those of FSIS method (CV of 4.6%~17.5%), recoveries from FSIS-Q were compared with those from KFSC method. FSIS-Q showed significantly higher recoveries of 83.1%~110.1% (P < 0.05) than those from KFSC method of 64.7%~107.4%. In addition, FSIS-Q showed a good linearity over the range of 2.5~200 ㎍/kg, and excellent sensitivities with limit of detection of 0.46~0.71 ㎍/kg and limit of quantification of 1.08~2.15 ㎍/kg. Although all the sample preparation methods turned out to be able to meet CODEX guideline for all the compounds, FSIS method and FSIS-Q validated in this study could be applied to screening and quantification for residues of febantel and its metabolites in rockfish muscle with efficient preparation procedures.

Keywords

Febantel; Fenbendazole; Rockfish; LC-MS/MS; Sample preparation; QuEChERS;

Citations & Related Records

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