Browse > Article
http://dx.doi.org/10.7847/jfp.2021.34.2.141

Genetic relatedness of white spot syndrome virus (WSSV) from imported frozen shrimp  

Choi, So Won (Department of Aquatic Life Medicine, Pukyong National University)
Baek, Eun Jin (Department of Aquatic Life Medicine, Pukyong National University)
Choi, Ji Yeong (Department of Aquatic Life Medicine, Pukyong National University)
Tae, Won Jun (Department of Aquatic Life Medicine, Pukyong National University)
Kim, Hyoung Soon (Department of Aquatic Life Medicine, Pukyong National University)
Park, Woo Seong (Department of Aquatic Life Medicine, Pukyong National University)
Kim, Min Jae (Department of Aquatic Life Medicine, Pukyong National University)
Kim, Kwang Il (Department of Aquatic Life Medicine, Pukyong National University)
Publication Information
Journal of fish pathology / v.34, no.2, 2021 , pp. 141-147 More about this Journal
Abstract
In this study, of the imported shrimps between 2017 and 2020, we investigated white spot syndrome virus (WSSV), covert mortality nodavirus (CMNV) and decapod iridescent virus 1 (DIV-1). Of the imported shrimps (a total of 29 groups), WSSV was detected as 31% (9/29) by nested PCR assay. And CMNV and DIV-1 were not identified in this study. To investigate the genetic relatedness of WSSV identified from imported shrimp, VR 14/15 region showed WSSV genomic variable loci was compared with reference isolates. Among the nine WSSV-positive samples, VR 14/15 region was amplified in only a sample (20-CH-1 isolate, imported from China in 2020). And the 20-CH-1 isolate showed 99.8% identity with WSSV-IN-05-01 which was reported in India in 2005, suggesting that those of WSSV have been spread from India to China. Furthermore, although the pathogenicity of WSSV identified from frozen shrimp was not evaluated, the international trade of diseased frozen shrimps could be led to the potential risk of virus transmission.
Keywords
Imported frozen shrimp; White spot syndrome virus; VR 14/15; Covert mortality nodavirs; Decapod iridescent virus 1;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Lo, C. F., Leu, J. H., Ho, C. H., Chen, C. H., Peng, S. E., Chen, Y. T., Chou, C. M., Yeh, P. Y., Huang, C. J., Chou, H. Y., Wang, C. H. and Kou, G.H.: Detection of baculovirus associated with white spot syndrome (WSBV) in penaeid shrimps using polymerase chain reaction. Diseases of Aquatic Organisms, 25: 133-141, 1996.   DOI
2 Hwang, S. D., Hwang, J. Y., Sohn, S., Kim, S. M., Kim, S. R., Kim, K. I., Kwon, M. J., Lee, K.Y., Kang, B. H. and Seo, J. S.: Genome data of shrimp acute hepatopancreatic necrosis disease causative Vibrio parahaemolyticus strains isolated from South Korea aquaculture farms. Data in Brief, 31: 105697, 2020.   DOI
3 Knibb, W., Le, C., Katouli, M., Bar, I. and Lloyd, C.: Assessment of the origin of white spot syndrome virus DNA sequences in farmed Penaeus monodon in Australia. Aquaculture, 494: 26-29, 2018.   DOI
4 Piamsomboon, P., Chaweepack, T., Han, J. E., Tang, K. F. and Wongtavatchai, J.: Genetic variation of white spot syndrome virus (WSSV) in Pacific white shrimp Litopenaeus vannamei (Boone 1931) culture of Thailand. Aquaculture Research, 49: 265-272, 2018.   DOI
5 Zhang, Q., Liu, Q., Liu, S., Yang, H., Liu, S., Zhu, L., Yang, B., Jin, J., Ding, L., Wang, X., Liang, Y., Wang, Q. and Huang, J.: A new nodavirus is associated with covert mortality disease of shrimp. Journal of General Virology, 95:2700-2709, 2014.   DOI
6 Balasubramanian, G., Sudhakaran, R., Syed Musthaq, S., Sarathi, M. and Sahul Hameed, A. S.: Studies on the inactivation of white spot syndrome virus of shrimp by physical and chemical treatments, and seaweed extracts tested in marine and freshwater animal models. Journal of fish diseases, 29: 569-572, 2006.   DOI
7 Chou, H., Huang, C., Wang, C., Chiang, H. and Lo, C.: Pathogenicity of a baculovirus infection causing white spot syndrome in cultured penaeid shrimp in Taiwan. Diseases of aquatic organisms, 23: 165-173, 1995.   DOI
8 Dieu, B. T. M., Marks, H., Siebenga, J. J., Goldbach, R. W., Zuidema, D., Duong, T. P., and Vlak, J. M. : Molecular epidemiology of white spot syndrome virus within Vietnam. Journal of General Virology, 85: 3607-3618, 2004.   DOI
9 FAO (Food and Agriculture Organization of the United Nations). The State of World Fisheries and Aquaculture (SOFIA). 2020. https://www.fao.org/fishery/static/Yearbook/YB2017_USBcard/index.htm
10 조미영, 김광일, 민은영, 정승희: 양식생물의 신종질병 및 국내 대응방안. 해양정책연구, 34: 67-88, 2019.   DOI
11 OIE, World Organization for Animal Health, Manual of Diagnostic Tests for Aquatic Animal. Chapter 2.2.8. Infection with white spot syndrome virus. 2021. https://www.oie.int/fileadmin/Home/eng/Health_standards/aahm/current/2.2.08_WSSV.pdf
12 Walker, P. J., & Mohan, C. V.: Viral disease emergence in shrimp aquaculture: origins, impact and the effectiveness of health management strategies. Reviews in aquaculture, 1: 125-154, 2009   DOI
13 Han, J. E., Lee, S. C., Park, S. C., Jeon, H. J., Kim, K. Y., Lee, Y. S., Park, S., Han, S. H., Kim, J. H. and Choi, S. K.: Molecular detection of Enterocytozoon hepatopenaei and Vibrio parahaemolyticus-associated acute hepatopancreatic necrosis disease in Southeast Asian Penaeus vannamei shrimp imported into Korea. Aquaculture, 517: 734812, 2020.   DOI
14 Flegel, T. W.: Major viral diseases of the black tiger prawn (Penaeus monodon) in Thailand. World Journal of Microbiology and Biotechnology, 13: 433-442, 1997.   DOI
15 Dupuy, J. W., Bonami, J. R. and Roch, P.: A synthetic antibacterial peptide from Mytilus galloprovincialis reduces mortality due to white spot syndrome virus in palaemonid shrimp. Journal of fish diseases, 27: 57-64, 2004.   DOI
16 Qiu, L., Chen, M. M., Wan, X.Y., Li, C., Zhang, Q. L., Wang, R. Y., Cheng, D. Y., Dong, X., Yang, B., Wang, X. H., Xiang, J. H. and Huang, J.: Characterization of a new member of Iridoviridae, Shrimp hemocyte iridescent virus (SHIV), found in white leg shrimp (Litopenaeus vannamei). Scientific reports, 7: 1-13, 2017.   DOI
17 Zwart, M. P., Dieu, B. T. M., Hemerik, L., and Vlak, J. M.: Evolutionary trajectory of white spot syndrome virus (WSSV) genome shrinkage during spread in Asia. PLoS One, 5: e13400. 2010.   DOI