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http://dx.doi.org/10.7847/jfp.2021.34.2.123

Phylogenetic classification and pathogenicity analysis of IHNV isolated from salmonids in Gangwon-do  

Lim, Jongwon (Department of Marine Biotechnology, College of Life Science, Gangneung-Wonju National University)
Go, Eunho (Department of Marine Biotechnology, College of Life Science, Gangneung-Wonju National University)
Hong, Suhee (Department of Marine Biotechnology, College of Life Science, Gangneung-Wonju National University)
Publication Information
Journal of fish pathology / v.34, no.2, 2021 , pp. 123-131 More about this Journal
Abstract
This study investigated the phylogenetic classification and pathogenicity of 6 infectious hematopoietic necrosis virus (IHNV) strains isolated from salmonid fish in Gangwon-do, Korea. Based on the nucleotide sequence of mid-G region, all six strains belong to J genotype, of which 5 are J-Nagano type and 1 J-Shizuoka type. In a challenge test, 5 isolates of J-Nagano type IHNV showed a various mortalities as 2 isolates induced a high mortality of 100% and the other 3 isolates induced mortalities of 50, 30, and 20% after intraperitoneal injection in rainbow trout (Oncorhynchus mykiss). Meanwhile no mortality was occurred by 1 isolate of J-Shizuoka type virus. Thus, it seems that there might be no relation between genotype and pathogenicity within IHNV J genotypes. This is contrary to previous studies where reported a higher pathogenicity of J-Shizuoka type virus than J-Nagano type virus in rainbow trout. Further examination will be required to clarify this since only one J-Shizuoka type virus was analyzed in this study.
Keywords
Infectious hematopoietic necrosis; Phylogenetic analysis; Genotype; Pathogenicity; Gangwon-do;
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