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http://dx.doi.org/10.7847/jfp.2021.34.1.023

Immune gene expression and protection effect against VHSV by injection of interferon regulatory factor 10 in zebrafish (Danio rerio)  

Kim, Hye Ji (Department of Aquatic Life Medical Sciences, Sunmoon University)
Kim, Jin Young (Department of Aquatic Life Medical Sciences, Sunmoon University)
Park, Jong Bin (Department of Aquatic Life Medical Sciences, Sunmoon University)
Lee, Ji Hyun (Department of Aquatic Life Medical Sciences, Sunmoon University)
Park, Jeong Su (Department of Aquatic Life Medical Sciences, Sunmoon University)
Kim, Hyoung Jun (OIE Reference Laboratory for VHS, National Institute of Fisheries Science)
Kwon, Se Ryun (Department of Aquatic Life Medical Sciences, Sunmoon University)
Publication Information
Journal of fish pathology / v.34, no.1, 2021 , pp. 23-29 More about this Journal
Abstract
Interferon regulatory factors (IRFs) are a family of transcription factors essential to the control of antiviral immune response, cell growth, differentiation and apoptosis. IRF10 of zebrafish (Danio rerio) was negative regulation of the interferonΦ1 and 3 response in vitro. In this study, we analyze the induction of in vivo immune response activation from the IRF10 gene of zebrafish and the protective effect against VHSV. As the results, the group inoculated with IRF10 expression vectors, there was no expression of IFNΦ1, suggestion that IRF10 may function as a negative regulator of IRF3, which binds to the IFNΦ1 promoter. And other types of interferon genes (IFNΦ2-4) are thought to have been activated, inducing to the expression of pro-inflammatory cytokine and Mx genes. As the results of challenge test performed at 14 days after inoculation of the expression vectors, the maximum survival rate [50% (1㎍ DNA) and 42.5% (10㎍ DNA)] for IRF10 group were recorded. Meanwhile, the survival rates of pcDNA3.1 and PBS as the control groups were 10% and 15%, respectively. This study suggests that the possibility that activation of IRF10 molecule could be exploited as a VHS control method.
Keywords
viral haemorrhagic septicaemia virus (VHSV); zebrafish (Danio rerio); interferon regulatory factor 10; IRF10; interferon; IFN;
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