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http://dx.doi.org/10.7847/jfp.2020.33.2.171

Production of monoclonal antibodies against marine birnavirus  

Kong, Kyoung-Hui (Department of Aqualife Medicine, College of Fisheries and Ocean Science, Chonnam National University)
Oh, Myung-Joo (Department of Aqualife Medicine, College of Fisheries and Ocean Science, Chonnam National University)
Kim, Wi-Sik (Department of Aqualife Medicine, College of Fisheries and Ocean Science, Chonnam National University)
Publication Information
Journal of fish pathology / v.33, no.2, 2020 , pp. 171-175 More about this Journal
Abstract
We developed and subsequently characterized mouse monoclonal antibodies (mAbs) against marine birnavirus (MABV). Eight hybridoma clones secreting mAbs against MABV were established. All eight mAbs (8G6, 11C3, 15E3, 17H6, 32A6, 35A7, 38B5, and 47E3) were reacted with viral protein 3 of MABV in MABV-infected CHSE-214, whereas, no reactivity was observed in normal CHSE-214 by western blot analysis. Moreover, these eight mAbs were strongly reacted with MABV, and no cross-reactivity has been observed against other five fish viruses (hirame rhabdovirus, infectious hematopoietic necrosis virus, nervous necrosis virus, spring viraemia of carp virus, and viral hemorrhagic septicemia virus), although five mAb (11C3, 15E3, 17H6, 32A6, and 38B5) reacted with both MABV and infectious pancreatic necrosis virus by enzyme linked immunosorbent assay (ELISA). These results indicate that the mAbs can be of value in MABV detection.
Keywords
Olive flounder; marine birnavirus; MABV; monoclonal antibody;
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