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http://dx.doi.org/10.7847/jfp.2020.33.2.119

Isolation Characteristics of causative agent of Streptococcosis and Serotype Changes of Streptococcus parauberis from olive flounder (Paralichthys olivaceus) in Jeju  

Kim, Kyeong Wook (Jeju Special Self-Governing Province Ocean and Fisheries Research Institute)
Yoo, Eun Ho (Jeju Special Self-Governing Province Ocean and Fisheries Research Institute)
Yang, Hye Young (Jeju Special Self-Governing Province Ocean and Fisheries Research Institute)
Kang, Bong Jo (Jeju Special Self-Governing Province Ocean and Fisheries Research Institute)
Publication Information
Journal of fish pathology / v.33, no.2, 2020 , pp. 119-125 More about this Journal
Abstract
During the period from 2003 to 2020, a total of 470 Streptococcus species were isolated from farmed flounder in Jeju. Out of 470 isolates, 92 (19.6%) isolates and 378 isolates (80.4%) were identified as Streptococcus iniae and S. parauberis, respectively by multiplex PCR assay. During that period, the percentage of S. iniae decreased from 56.9% in 2003 to 0.0% in 2020 whereas that of S. parauberis increased from 43.1% in 2003 to 100% in 2020. In the PCR assay for serotyping, the isolated S. parauberis showed 3 subserotypes, Ia (34.9%), Ib/Ic (46.3%) and II (18.8%). In 2003 and 2004, serotype II was dominant at 59.1% and 50.0% of isolation rates, however between 2005 and 2009, subserotype Ib/Ic was dominant (57.6%, 86.0%, 84.6%, 57.9%, and 83.3%). After 2010, except for 2015, subserotype Ia was the most dominant one. In the last 3 years (2018 to 2020), subserotype Ia was most abundant (70%), followed by subserotype Ib/Ic (16-30%). Serotype II was not isolated in 2018, but in 2019 and 2020, it showed an increased tendency to 3.4% and 16.7%, respectively. It is believed that continuous monitoring is necessary for research on counter-measures against streptococcosis of flounder.
Keywords
olive flounder; streptococcosis; Streptococcus iniae; Streptococcus parauberis; serotype;
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