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http://dx.doi.org/10.7847/jfp.2019.32.2.059

Development of monoclonal antibodies against viral hemorrhagic septicemia virus (VHSV, genotype IVa), the causative agent of VHS  

Kong, Kyoung-Hui (Department of Aqualife Medicine, College of Fisheries and Ocean Science, Chonnam National University)
Oh, Myung-Joo (Department of Aqualife Medicine, College of Fisheries and Ocean Science, Chonnam National University)
Jang, Min-Seok (South Sea Fisheries Research Institute, National Institute of Fisheries Science (NIFS))
Kim, Choon-Sup (Enbiogene)
Kim, Wi-Sik (Department of Aqualife Medicine, College of Fisheries and Ocean Science, Chonnam National University)
Publication Information
Journal of fish pathology / v.32, no.2, 2019 , pp. 59-67 More about this Journal
Abstract
We developed and subsequently characterized mouse antibodies (MAbs) against viral hemorrhagic septicemia virus (VHSV, genotype IVa), the causative agent of VHS. Five hybridoma clones secreting MAbs against VHSV were established. The MAbs recognized the glycoprotein (MAbs 2C10, 18H4, 23H6, and 30B7) and nucleocapsid protein (15E10) of VHSV by western blot analysis. All five MAbs reacted with VHSV-infected cells and tissue homogenates of VHSV-infected olive flounder (Paralichthys olivaceus) by western blot analysis. Whereas, no reactivity was observed in normal cells and tissue homogenates of normal olive flounder. Moreover, these MAbs reacted with VHSV, but did not react with other fish viruses (infectious hematopoietic necrosis virus, hirame rhabdovirus, spring viraemia of carp virus, infectious pancreatic necrosis virus, marine birnavirus, and nervous necrosis virus) by enzyme linked immunosorbent assay (ELISA). These results indicate that the MAbs are specific to VHSV and can be of value in VHSV detection.
Keywords
Olive flounder; viral hemorrhagic septicemia virus; genotype IVa; VHSV; monoclonal antibody;
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