Browse > Article
http://dx.doi.org/10.7847/jfp.2018.31.2.087

The first case report on Vibrio atlanticus infection in cultured tiger puffer, Takifugu rubripes  

Lee, Nam-Sil (Fish Pathology division, National Fisheries Research and Development Institute)
Cho, Miyoung (Fish Pathology division, National Fisheries Research and Development Institute)
Jung, Sung-hee (Fish Pathology division, National Fisheries Research and Development Institute)
Won, Kyoungmi (Fish Pathology division, National Fisheries Research and Development Institute)
Publication Information
Journal of fish pathology / v.31, no.2, 2018 , pp. 87-92 More about this Journal
Abstract
A case of disease was occurred in tiger puffer, Takifugu rubripes, reared in circulated system (water temperature was about $20^{\circ}C$) on land in Korea. Diseased fish showed a unique external sign, the cloudy skin ulcer in various sizes. We investigated the cause of disease, and isolated one pure cultured bacterium, TP01. This had 99.7% homology with Vibrio atlanticus by sequence analysis of the 16S rRNA and rpoA genes. TP01 showed some differences with other reported V. atlanticus strains in the biochemical characteristics as like Voges-Proskauer test and gelatin hydrolysis.
Keywords
Skin ulcer; Tiger puffer; Takifugu rubripes; Vibrio; Vibrio atlanticus;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Abe, T.:Taxonomic studies on the puffers (Tetraodontidae, Teleostei) from Japan and adjacent regions,V, Synopsis of the puffers from Japan and adjacent regions. Bull. Biogeograph. Soc. Jap., 14, 1-15, 89-140, 1949.
2 Ana, L. D., Roxana, B-.H., Ilse, C., Sabela B.., Paul de Vos and Jesus L. R.:Vibrio atlanticus sp. nov. and Vibrio artabrorum sp. nov., isolated from the clams Ruditapes philippinarum and Ruditapes decussatus. Int. J. Syst. Evol. Microbiol., 61, 2406-2411, 2011.   DOI
3 Hashimoto, E., Miyadai, T., Ohtani, M. and Oh, M.J.: Proliferation of kuchijirosho causative agent in a fugu-derived cell line. J. Fish Dis., 31: 443-339, 2008   DOI
4 Hujita, S., Kmiyama T. and Yogata T.:An artificial ovulation of the puffer, Fugu niphobles, by hormone injection, with a description of a practical incubation method with strong aeration. Aqu. Sci., 14:31-36, 1966.
5 Japanease Sea National Fisheries Research Institute (JSNFRI); http://jsnfri.fra.affrc.go.jp/pref/fukui/2000/pdf/43-46.pdf, 2000
6 Mafaddin, J.F.: Biochemical tests for identification of medical bacteria. 3ed. Awolter Klvwer Company, 2000.
7 Japanese Sea National Fisheries Research Institute (JSNFRI); http://jsnfri.fra.affrc.go.jp/pref/fukui/2002/pdf/61-63.pdf, 2002
8 Kusuda, R. and Kawai, K.:Bacterial diseases of cultured marine fish in Japan. Fish Pathol., 33, 221-227, 1998.   DOI
9 Lin, Z., Wang, H., Yu, C., Lv, F., Liu, H. and Zhang Tao.: Commercial production of tiger puffer (Takifugu rubripes) in Winter using a recirculating aquaculture system. Journal of Ocean Univerity of China, 16, 107-113, 2017.   DOI
10 Han, K-.N.: Development of eggs, larvae and juveniles of the puffer, Takifugu rubripes reared in the Laboratory. J. Aquaculture, 12:255-266, 1999.
11 Ministry of oceans and fisheries (MOF): Oceans and fisheries statistical system, statistics by fishery and by kind. www.mof.go.kr/statPortal/, 2018.
12 Mohi, M.M., Kuratani, M., Miyazaki, T. and Yoshida, T.:Histopathological studies on Vibrio harveyi infected tiger puffer, Takifugu rubripes (Temminck et Schlegel), cultured in Japan. J. Fish Dis., 33, 833-840, 2010.   DOI
13 Thompson, F.L. Austin, B. and Swings, J.: The biology of vibrios, Taxonomy of the vibrios, p.29-43, ASM Press, Washington, DC., 2006.
14 Muroga, K., Tanasomwang, V. and Momoyama, K.: Vibrio anguillarum infection in tiger puffer (Takifugu rubripes) fingerlings. Fish Pathol., 22, 29-30, 1987.   DOI
15 Muroga, K.: Viral and bacterial diseases in larval and juvenile marine fish and shellfish: a review. Fish Pathol. 30, 71-85, 1995.   DOI
16 Nakanishi, T. and Matsuura, Y.:Current status and issues of fish diseases in Japan. J. Jap. Vet. Medi. Assoc., 69 27-35, 2016.   DOI
17 National Institute Fisheries Sciences (NIFS): Aquaculture Manual of River Puffer (Takifugu obscurus), Serial Number ED-2006-AQ-001, 2006.
18 Thompson, F.L., Gevers, D., Thompson, C.C., Dawyndt, P., Naser, S., Hoste, B., Munn C.B. and Swings J.: Phylogeny and Molecular Identification of Vibrios on the Basis of Multilocus Sequence Analysis. Applied and Environmental Microbiology. 71, 5107-5115, 2005.   DOI
19 Thompson, F.L., Hoste, B., Vandemeulebroecke, K. and Swings, J..: Genomic diversity amongst Vibrio isolates from different sources determined by fluorescent amplified fragment length polymorphism. Syst. Appl. Microbiol. 24: 520-538, 2001.   DOI
20 Thompson, F.L., Iida, T. and Swings, J.: Biodiversity of vibrios. Microbiol. Mol. Biol. Rev., 64:403-431, 2004.
21 Tomoo, S., Kita-Tukamoto, K. and Thompson, F.L.: Inferring the Evolutionary History of Vibrios by means of Multilocus sequence analysis. Journal of Bacteriology. 189, 7932-7936, 2007.   DOI
22 Wan, Z.Z., Gao, T.X., Zhang, X.M., Chen, C. and Yu, C.H.: Histological study on the digestive system development of Takifugu rubripes larvae and juvenile. J. Ocean Univ. China, 5, 39-44, 2006.   DOI