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http://dx.doi.org/10.7847/jfp.2018.31.1.041

Reduction of dissolved hydrogen sulfide and mortality of white leg shrimp, Litopenaeus vannamei by Bacillus spp. microorganisms  

Choi, Jun-Ho (Department of Aquatic Life Medicine, College of Ocean Science and Technology, Kunsan National University)
Lee, Ji-Hoon (Department of Aquatic Life Medicine, College of Ocean Science and Technology, Kunsan National University)
Park, Jung-Jin (Department of Aquatic Life Medicine, College of Ocean Science and Technology, Kunsan National University)
Lee, Min-Sun (Department of Aquatic Life Medicine, College of Ocean Science and Technology, Kunsan National University)
Bae, Jun-Sung (Department of Aquatic Life Medicine, College of Ocean Science and Technology, Kunsan National University)
Shin, Dong-Hun (Shindong Control Center For Aquatic Animal Diseases)
Park, Kwan Ha (Department of Aquatic Life Medicine, College of Ocean Science and Technology, Kunsan National University)
Publication Information
Journal of fish pathology / v.31, no.1, 2018 , pp. 41-48 More about this Journal
Abstract
The utility of Bacillus spp. organisms for reduction of dissolved hydrogen sulfide ($H_2S$) in white leg shrimp (Litopenaeus vannamei) culture was tested with different combinations of Bacillus spp. microorganisms: combination A (B. subtilis + B. licheniformis); combination B (B. licheniformis + B. amyloliquefaciens); combination C (B. subtilis + B. licheniformis + B. amyloliquefaciens). Of these 3 combinations, C was effective in few hours after addition whereas B needed longer time to be effective. The $H_2S-reducing$ effect of combination C was dependent on the amount of microorganisms added to $H_2S-containing$ test solution. Exposure of white leg shrimp to $H_2S$ at 8 mg/L for 7 days led to survival of 80% and 1 mg/L for 14 days it was 82.5%. The survival rate was 97.5% when combination C was simultaneously added to shrimp tanks during $H_2S$ exposure at 1 mg/L for 14 days. It was demonstrated that combination C microorganisms (B. subtilis + B. licheniformis + B. amyloliquefaciens) can reduce dissolved $H_2S$ concentrations, and this effect can be utilized to protect white leg shrimp from $H_2S$ toxicity.
Keywords
Bacillus spp.; Dissolved $H_2S$; Litopenaeus vannamei; White leg shrimp;
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