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http://dx.doi.org/10.7847/jfp.2017.30.2.149

Production of monoclonal antibodies against viral hemorrhagic septicemia virus (VHSV, genotype IVa) from olive flounder  

Jeong, Ha-Na (Department of Aqualife Medicine, College of Fisheries and Ocean Science, Chonnam National University)
Jang, Min-Seok (Department of Aqualife Medicine, College of Fisheries and Ocean Science, Chonnam National University)
Oh, Myung-Joo (Department of Aqualife Medicine, College of Fisheries and Ocean Science, Chonnam National University)
Kim, Wi-Sik (Department of Aqualife Medicine, College of Fisheries and Ocean Science, Chonnam National University)
Publication Information
Journal of fish pathology / v.30, no.2, 2017 , pp. 149-154 More about this Journal
Abstract
Mouse monoclonal antibodies (MAbs) were produced by using viral hemorrhagic septicemia virus (VHSV, genotype IVa) as an immunogen, isolated from diseased olive flounder (Paralichthys olivaceus). Four hybridoma clones secreting MAbs against VHSV were established. The MAbs were recognized the nucleoprotein (MAb 4), phosphoprotein (MAb 1) and matrix protein (MAbs 2 and 3) of VHSV by western blot analysis. Among them, the MAbs 1 and 4 strongly reacted with the VHSV-infected FHM cells, but not normal FHM cells. In enzyme linked immunosorbent assay, the four MAbs reacted with the VHSV, but not different six fish viruses (infectious hematopoietic necrosis virus, hirame rhabdovirus, spring viraemia of carp virus, infectious pancreatic necrosis virus, marine birnavirus and nervous necrosis virus). These results indicate that the MAbs are useful for diagnosis of VHSV infection.
Keywords
Viral hemorrhagic septicemia virus; VHSV; Monoclonal antibody; Olive flounder;
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