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http://dx.doi.org/10.7847/jfp.2017.30.1.001

Monitoring of viruses in cultured walleye pollock Gadus chalcogrammmus  

Nam, U-Hwa (Department of Marine Bioscience, Gangneung-Wonju National University)
Jeon, Chan-Hyeok (Life Resources Management Division, Korea Fisheries Resources Agency)
Seo, Hyun-Joon (Department of Marine Bioscience, Gangneung-Wonju National University)
Choi, Da-Young (Department of Marine Bioscience, Gangneung-Wonju National University)
Seo, Joo-young (Gangwon Coldsea Fisheries Resources Center)
Kwon, O-Nam (GABI Co. Ltd.)
Kim, Wi-Sik (Department of Aqualife Medicine, Chonnam National University)
Kim, Jeong-Ho (Department of Marine Bioscience, Gangneung-Wonju National University)
Publication Information
Journal of fish pathology / v.30, no.1, 2017 , pp. 1-9 More about this Journal
Abstract
This study was conducted to monitor the prevalence of viral hemorrhagic septicemia virus (VHSV), nervous necrosis virus (NNV) and marine birnavirus (MABV) in cultured walleye pollock Gadus chalcogrammus by RT-PCR. All of the viruses tested were not detected by one-step PCR in 62 spleen sample sets, except for NNV in one brain sample set (1/55). By two-step PCR, VHSV was detected in 51.6%(32/62) and NNV was detected in 1.6%(1/62) spleen sample set, but MABV was not detected. In the brain sample sets, the detection rate of NNV was 3.6%(2/55). VHSV and NNV were detected for the first time in cultured walleye pollock in this study. However, the titers of viruses in these sample sets are thought to be very low, because most of the positive sample sets were detected by two-step PCR and none of the fish showed any clinical symptoms of each virus. Continuous monitoring, subsequent virus isolation and validation of carrier fish will be necessary.
Keywords
Walleye pollock; Gadus chalcogrammus; Viral hemorrhagic septicemia virus; Nervous necrosis virus; RT-PCR;
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Times Cited By KSCI : 8  (Citation Analysis)
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1 Samuelsen, O.B., Nerland, A.H., Jorgensen, T., Schroder, M.B., Svasand, T. and Bergh, O.: Viral and bacterial diseases of Atlantic cod Gaud morhua, their prophylaxis and treatment: a review. Dis. Aquat. Org., 71: 239-254, 2006.   DOI
2 Shetty, M., Maiti, B., Santhosh, K.S., Venugopal, M. N., and Karunasagar, I.: Betanodavirus of Marine and Freshwater Fish: Distribution, Genomic Organization, Diagnosis and Control Measures. Indian J. Virol., 23: 114-123, 2012.   DOI
3 Schlotfeldt, H.J., Ahne, W., Jorgensen, P.E.V. and Glende, W.: Occurrence of viral haemorrhagic septicaemia in turbot (Scophthalmus maximus)-a natural outbreak. Bull. Eur. Assoc. Fish Pathol., 11: 105-107, 1991.
4 Shida, O., Hamatsu, T., Nishimura, A., Suzaki, A., Yamamoto, J., Miyashita, K. and Sakurai, Y.: Interannual fluctuations in recruitment of walleye Pollock in the Oyashio region related to environmental changes. Deep-Sea Res. II, 54, 2822-2831, 2007.   DOI
5 Stone, D.M., Way, K. and Dixon, P.F.: Nucleotide sequence of the glycoprotein gene of viral haemorrhagic septicaemia (VHS) viruses from different geographical areas: a link between VHS in farmed fish species and viruses isolated from North Sea cod (Gadus morhua L.). J. Gen. Virol., 78: 1319-1326, 1997.   DOI
6 Suebsing, R.: Development and evaluation of a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for detecting fish viruses in Korea. Ph.D. Thesis, Gangneung-Wonju national university, Gangneung, Korea, 2012.
7 Suzuki. S., Hosono, N. and Kusuda, R.: Detection of aquatic birnavirus gene from marine fish using a combination of reverse transcription and nested PCR. J. Mar. Biotechnol., 5: 205-209, 1997.
8 Olesen. N.J.: Sanitation of viral haemorrhagic septicaemia (VHS). J. Appl. Ichthyol., 14: 173-177, 1998.   DOI
9 강수경, 박정호, 김수암: 1970-1990년대 동해에서 어획된 명태(Theragra chalcogramma)의 체장에 따른 체급별 어획 마릿수 추정. 한국수산과학회지, 16: 445-453, 2013.
10 Tamura, K., Stecher, G., Peterson, D., Filipski, A., and Kumar, S.: MEGA6: Molecular Evolutionary Genetics Analysis Version 6.0. Mol. Biol. Evol., 30: 2725-2729, 2013.   DOI
11 김수미, 박수일: 우리나라 연근해 자연산 해수 어종에서 Viral Hemorrhagic Septicemia Virus(VHSV)의 검출. 한국어병학회지, 17: 1-10, 2004.
12 김선래, 김은미: 한국의 러시아 명태 수입과 러시아수산업투자 고찰. 한국 시베리아연구, 18: 55-78, 2014.
13 박현경, 김승민, 이다원, 전려진, 정준범: 2014년 제주도 양식 넙치, Paralichthys olivaceus에 대한 VHS 및 RSIVD 모니터링. 수산해양교육연구, 27: 879-889, 2015.
14 김위식, 김종오: 능성어(Epinephelus septemfasciatus)양식장에서의 바이러스성신경괴사증(VNN) 예방대책. 한국수산과학회지, 48: 403-410, 2015.
15 김진도, 김석렬, 정성주, 김영진, 정성태, 최태진, 박성우, 오명주 : 홍민어 Sciaenops ocellatus 에서의 바이러스성 신경괴사증 viral nervous necrosis. 한국어병학회지, 14: 91-95, 2001.
16 김춘섭, 김위식, Nishizawa Toyohiko, 오명주: 능성어 양식장에서의 viral nervous necrosis (VNN) 발생양상. 한국어병학회지, 25: 111-116, 2012.
17 서주영, 권오남: 국내 명태 Theragra chalcogramma 자연 채란과 난황흡수까지의 난 발생. 한국산학기술학회논문지, 18: 49-54, 2017.
18 신용민: 명태어업과 중부베링해 명태자원보존관리 협약에 대한 고찰. 해양비즈니스학회지, 15, 81-118, 2010.
19 안상중, 조미영, 지보영, 박명애: 아시아에서 분리된 viral haemorrhagic septicaemia virus (VHSV) isolates의 계통분석학적 비교. 한국어병학회지, 26: 149-161, 2013.
20 양윤선, 강수경, 김수암, 김순송: 명태(Theragra chalcogramma)이석 내 산소동위원소 조성과 서식 수온 특성. Ocean Polar Res, 30: 249-258, 2008.   DOI
21 오태기, 사쿠라모토 카즈미, 이상고: 동해안에 있어서의 명태 어획량 변동과 산란장의 수온과의 관계. 한국수산자원학회지, 6: 2-13, 2004.
22 이월라, 윤현미, 김석렬, 정성주, 오명주: 남,서해안과 동중국해 자연산 어류에서 Viral hemorrhagic septicemia virus (VHSV) 검출. 한국어병학회지, 20: 201-209, 2007.
23 조미영, 박경현, 지보영, 김진우: 2005년부터 2007년 사이 양식 넙치, Paralichthys olivaceus를 대상으로 한 어류바이러스 검출에 대한 통계 자료. 한국어병학회지, 23: 155-163, 2010.
24 Jensen, M.H.: Research on the virus of Egtved disease. Ann. NY Acad. Sci., 126: 422-426, 1965.
25 Cha, S.J., Do, J.W., Lee, N.S., An, E.J., Kim, Y.C., Kim, J.W. and Park, J.W.: Phylogenetic analysis of betanodaviruses isolated from cultured fish in Korea. Dis. Aquat. Org., 77: 181-189, 2007.   DOI
26 Dixon, P.F., Avery, S., Chambers, E., Feist, S., Mandhar, H., Parry, L., Stone, D.M., Strommen, H.K., Thurlow, J.K., Lui, C.T. and Way, K.: Four years of monitoring for viral haemorhagic septicaemia virus in marine waters around the United Kingdom. Dis. Aquat. Org., 54: 175-186, 2003.   DOI
27 Dixon, P.F., Feist, S., Kehoe, E., Parry, L., Stone, D.M., Way, K.: Isolation of viral haemorrhagic septicaemia virus from Atlantic herring Clupea harengus from the English Channel. Dis. Aquat. Org., 30: 81-89, 1997.   DOI
28 Jensen, N.J., Bloch, B., Larsen, J.L.: The ulcus-syndromein cod (Gadus morhua) III. A preliminary virological report. Nord. Vet. Med., 31: 436-442, 1979.
29 Katou, t.: Alaska Pollack Trade Relation between Japan and Korea. The Journal of Fisheries Business Administration, 33: 109-119, 2002.
30 Mao, M.G., Wen, S.H., Li, H., Jiang, J.L., Jiang, Z.Q., Li, X., Sun, H. and Lu, H.Q.: Evidence for and characterization of nervous necrosis virus infection in Pacific cod (Gadus macrocephalus). Arch. Virol,, 160: 2237-2248, 2015.   DOI
31 Nishizawa, T., Mori, K.i., Furuhashi, M., Nakai, T., Furusawa, I., and Muroga, K.: Comparison of the coat protein genes of five fish nodaviruses, the causative agents of viral nervous necrosis in marine fish. J. Gen. Virol., 76: 1563-1569. 1995.   DOI
32 Meyers, T.R., Short, S. and Lipson, K.: Isolation of the North American strain of viral hemorrhagic septicemia virus (VHSV) associated with epizootic mortality in two new host species of Alaskan marine fish. Dis. Aquat. Org., 38: 81-86, 1999.   DOI