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http://dx.doi.org/10.7847/jfp.2015.28.1.027

Detection and Analysis of the Potential Risk of VHSV in Bivalves in Korea  

Choi, Jae Chan (Department of Aquatic Life Medicine, Pukyong National University)
Kim, Young Chul (Department of Aquatic Life Medicine, Pukyong National University)
Choi, Hwan Jun (Department of Aquatic Life Medicine, Pukyong National University)
Park, Jeon Oh (Department of Aquatic Life Medicine, Pukyong National University)
Jeong, Hyun Do (Department of Aquatic Life Medicine, Pukyong National University)
Publication Information
Journal of fish pathology / v.28, no.1, 2015 , pp. 27-35 More about this Journal
Abstract
VHSV is a major viral agent that affects freshwater and marine fish, causing serious economic losses in aquaculture in the world. Due to their filter-feeding activity, bivalve mollusks may act as viral transmitters after accumulation of the fish viruses released into seawater from infected fish. Amplification by RT-PCR was carried out to investigate the presence of VHSV in pacific oysters (Crassotrea gigas) and blue mussels (Mytilus edulis), inhabiting regions around aquatic farms in Korea. Primers designed from conserved regions of VHSVs allowed us to detect four different types of VHSV in a single PCR. Twenty two of the eighty four samples showed positive results of VHSV in a 2-step RT-PCR. Using six positive samples from three different regions in Korea, we cloned and sequenced the glycoprotein (G) gene (467-bp long) of VHSVs. Genetic analysis of the VHSVs detected in shellfish in various geographical areas of Korea showed highly restricted results to VHSV type Iva. This was in agreement with the reports showing only a single genotype of VHSV (Iva genotype) in outbreaks in cultured or wild fish in Korea. Consequently, we investigated VHSVs carried by bivalve mollusks inhabiting the vicinity of aquatic farms, and revealed correlationship between the type of viral accumulated in shellfish by filter-feeding, and those detected in disease outbreaks in fish.
Keywords
VHSV; Bivalves; Shellfish; Glycoprotein gene; Genotype;
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  • Reference
1 Atmar, R.L., Metcalf T.G., Neill, F.H. and Estes, M.K.: Detention of enteric viruses in oyster by using the polymerase chain reaction. Environ. Microbiol., 59: 631-635, 1993.
2 Brudeseth, B.E. and Evensen, O.: Occurrence of viral haemorrhagic septicaemia virus (VHSV) in wild marine fish species in the coastal regions of Norway. Dis. Aquat. Org., 52: 21-28, 2002.   DOI
3 Brunson, R., True, K. and Yancey, J.: VHS virus isolated at Makah National Fish Hatchery. Am. Fish. Soc. Fish Health Sect. Newsl., 17: 3-4, 1989.
4 Cox, B. and Hedrick, R.: VHS virus in Pacific sardines and mackerel. Am. Fish. Soc. Fish Health Sect. Newsl., 29: 6, 2001.
5 Dixon, P.F., Feist, S., Kehoe, E., Parry, L., Stone, D.M. and Way, K.: Isolation of viral haemorrhagic septicaemia virus from Atlantic herring Clupea harengus from the English Channel. Dis. Aquat. Org., 30: 81-89, 1997.   DOI
6 Gerba, C.P. and Goyal, S.M.: Detection and occurrence of enteric viruses in shellfish: a review. J. Food. Protect., 41: 743-754, 1978.   DOI
7 Goyan, S.M., Gerba, C.P. and Melnick, J.L.: Human entero viruses in oysters and their overlaying waters. Appl. Environ. Microbiol., 37: 572-575, 1979.
8 Meyers, T.R., Sullivan, J., Emmenegger, E., Follett, J., Short, S. and Batts, W.N.: Identification of viral hemorrhagic septicemia virus isolated from Pacific cod Gadus macrocephalus in Prince William Sound, Alaska, USA. Dis. Aquat. Org., 12: 167-175, 1992.   DOI
9 Mortensen, H.F., Heuer, O.E., Lorenzen, N., Otte, L. and Olesen, N.J.: Isolation of viral haemorrhagic septicaemia virus (VHSV) from wild marine fish species in the Baltic sea, Kattegat, Skagerrak and the North Sea. Virus. Res., 63: 95-106, 1999.   DOI   ScienceOn
10 Mortensen, S.: Passage of infectious pancreatic necrosis virus (IPNV) through invertebrates in an aquatic food chain. Dis. Aquat. Org., 16: 41-45, 1993.   DOI
11 Suzuki, S. and Nojima, M.: Detection of a marine birnavirus in wild molluscan shellfish species from Japan. Fish. Pathol., 34: 121-125, 1999.   DOI
12 Snow, M., Bain, N., Black, J.,Taupin, V., Cunningham, C., King, J., Skall, H. and Raynard, R.: Genetic population structure of marine viral haemorrhagic septicaemia virus (VHSV). Dis. Aquat. Org., 61: 11-12, 2004.   DOI   ScienceOn
13 Smail, D.A.: Viral haemorrhagic septicaemia. Fish Diseases and Disorders, pp. 123-147, Volume 3: Viral, Bacterial and Fungal Infections, CAB International, New York, NY, 1999.
14 Smail, D.A.: Isolation and identification of viral haemorrhagic septicaemia (VHS) viruses from cod Gadus morhua with the ulcus syndrome and from haddock Melanogrammus aeglefinus having skin haemorrhages in the North Sea. Dis. Aquat. Org., 41: 231-235, 2000.   DOI
15 Snow, M., Cunningham, C.O., Melvin, W.T. and Kurath, G.: Analysis of the nucleoprotein gene identifies distinct lineages of viral haemorrhagic septicaemia virus within the European marine environment. Virus. Res., 63: 35-44, 1999.   DOI   ScienceOn
16 Jensen, N.J., Bloch, B. and Larsen, J.L.: The ulcus-syndrome in cod (Gadus morhua). III. A preliminary virological report. Nord. Vet. Med., 31: 436-442, 1979.
17 Traxler, G.S. and Kieser, D.: Isolation of the North American strain of viral hemorrhagic septicemia virus (VHSV) from herring (Clupea harengus pallasi) in British Columbia. Am. Fish. Soc. Fish Health Sect. Newsl., 22: 8, 1994.
18 Vazquez-Boucard, C., Alvarez-Ruiz, P., Escobedo-Fregoso, C., Anguiano-Vega, G., Duran-Avelar, MdJ., Pinto, V.S. and Escobedo-Bonilla, C.M.: Detection of white spot syndrome virus (WSSV) in the Pacific oyster Crassostrea gigas. J. Invest. Pathol., 104: 245-247, 2010.   DOI