Browse > Article
http://dx.doi.org/10.7847/jfp.2014.27.1.001

cDNA microarray analysis of viral hemorrhagic septicemia infected olive flounder, Paralichthys olivaceus: immune gene expression at different water temperature  

Kim, Jin-Ung (Yeosu center, Ocean & Fishery Science Institute)
Jung, Sung-Ju (Department of Aqualife Medicine, College of Fisheries and Ocean Science, Chonnam National University)
Publication Information
Journal of fish pathology / v.27, no.1, 2014 , pp. 1-9 More about this Journal
Abstract
The olive flounder, Paralichthys olivaceus is susceptible to viral hemorrhagic septicaemia virus (VHSV) at $15^{\circ}C$ but no mortality at $20^{\circ}C$ even though the virus can grow well in vitro at $20^{\circ}C$. Thus, we designed an experiment to know immune response of olive flounder against VHSV when the host reared at $15^{\circ}C$ or $20^{\circ}C$. cDNA microarray analysis was performed to compare the gene expression patterns of the kidney cells between the host reared at $15^{\circ}C$ or $20^{\circ}C$. The expression of MHC class I, IL-8, myeloperoxidae and endonuclease G-like having function for the antigen presentation and chemokine-factor were up-regulted both the $15^{\circ}C$ and $20^{\circ}C$ during VHSV infection. MHC class II gene existing on antigen-presenting cells and B cell lymphocytes, immunoglobulin (Ig) genes and phagocytosis related genes were down-regulated at $15^{\circ}C$ but highly expressed at $20^{\circ}C$. It can be thought that innate immune related antigen presentation by MHC class I and phagocytosis reaction against VHSV are efficiently occur both the temperature but macrophage or B cell related antigen presentation via MHC class II fails to induce downstream immune reactions (adaptive immunity) to make antibody, and it can be one of the reason that causes high mortality only at $15^{\circ}C$.
Keywords
VHSV; Temperature susceptibility; Microarray; Antigen presentation; Immunoglobulin;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 Dang, T.L., Yasuike, M., Hirono, I., Kondo, H. and Aoki, T.: Transcriptional profile of red seabream iridovirus in a fish model as revealed by viral DNA microarrays. Virus Genes, 35: 449-461, 2007.   DOI
2 Avunje, S., Kim, W.S., Park, C.S., Oh, M,J, and Jung, S,J.: Toll-like receptors and interferon associated immune factors in viral haemorrhagic septicaemia virus- infected olive flounder (Paralichthys olivaceus). Fish Shellfish Immunol., 31: 407-414, 2011.   DOI
3 Avunje, S., Kim, W.S., Oh, M,J,, Choi, I. and Jung, S.J.: Temperature-dependent viral replication and antiviral apoptotic response in viral haemorrhagic septicaemia virus (VHSV)-infected olive flounder (Paralichthys olivaceus). Fish Shellfish Immunol., 32: 1162-1170, 2012.   DOI
4 Avunje, S., Oh, M.J. and Jung, S.J.: Impaired TLR2 and TLR7 response in olive flounder infected with viral haemorrhagic septicaemia virus at host susceptible $15^{\circ}C$ but high at non-susceptible $20^{\circ}C$. Fish Shellfish Immunol., 34: 1236-1243, 2013.   DOI
5 Brudeseth, B.E., Castric, J. and Evensen, O.: Studies on pathogenesis following single and double infection with viral hemorrhagic septicemia virus and infectious hematopoietic necrosis virus in rainbow trout (Oncorhynchus mykiss). Vet Pathol 39: 180-189, 2002.   DOI
6 Byon, J.Y., Ohira, T., Hirono, I. and Aoki, T.:Use of a cDNA microarray to study immunity against viral hemorrhagic septicemia (VHS) in Japanese flounder (Paralichthys olivaceus) following DNA vaccination. Fish Shellfish Immunol., 18: 135-147, 2005.   DOI   ScienceOn
7 Friend, S.H., Bernards, R., Rogeij, S., Weinberg, R.A., Rapaport, J.M., Alberts, D. M. and Dryja R.P.: A human DNA segment with properties of the gene that predisposes to retinoblastoma and osteosarcoma. Nature, 323: 643-646, 1986.   DOI   ScienceOn
8 Hanington, P.C., Barreda, D.R. and Belosevic M.A.: novel hematopoietic granulin induces proliferation of goldfish (Carassius auratus L.) macrophages. J Biol Chem., 281: 9963-9970, 2006.   DOI
9 Hass-Kogan, D.A., Kogan, S. C., Levi, D., Dazin, P., Ang, A.T., Fung, Y.K. and Israel, M.A.: Inhibition of apoptosis by the retinoblastoma gene product. J EMBO., 14: 461-472, 1995.
10 Heinecke, J.W., Li, W., Daenke H.L. 3rd and Goldstein J.A.: Dityrosine, a specific marker of oxidation, is synthesized by the myeloperoxidase hydrogen peroxide system of human neutrophils and macrophage. J Biol Chem., 268: 4069-4077, 1993.
11 Hung, C.H., Huang, H.R., Huang, C.J., Huang, F.L. and Chang G.D.: Purification and cloning of carp nephrosin, a secreted zinc endopeptidase of the astacin family. J Biol Chem., 272: 13772-13778, 1997.   DOI
12 Hwang, E.S., Park C.G. and Cha C.Y.: Immune responses to viral infection. Immune Network, 4: 73-80, 2004.   DOI
13 Miller, T.A., Rapp, J., Wastlhuber, U., Hoffmann, R.W. and Enzmann, P.J. Rapid and sensitive reverse transcriptase-polymerase chain reaction based detection and differential diagnosis of fish pathogenic rhabdoviruses in organ samples and cultured cells. Dis Aquat Org., 34: 13-20, 1998.   DOI   ScienceOn
14 Isshiki, I., Nishizawa, T., Kobayashi, T., Nagano, T. and Miyazaki T.: An outbreak of VHSV (viral haemorrhagic septicemia) infection in farmed Japanese flounder Paralichthys olivaceus in Japan. Dis Aquat Org., 47: 87-99, 2001.   DOI
15 Kim, S.M., Lee, J.I., Hong, M.J., Park, H.S. and Park S.I.: Genetic relationship of the VHSV (viral hemorrhagic septicemia virus) isolated from cultured olive flounder, Paralichthys olivaceus in Korea. J Fish Pathol., 16: 1-12, 2003.   과학기술학회마을
16 Sano, M., Ito, T., Matsuyama, T., Nakayasu, C. and Kurita J.: Effect of water temperature shifting on mortality of Japanese flounder Paralichthys olivaceus experimentally infected with viral hemorrhagic septicemia virus. Aquaculture, 286: 254-258, 2009.   DOI   ScienceOn
17 Levine, T.P. and Chain, B.M.: The cell biology of antigen processing. Crit Rev Biochem Mol Biol., 26: 439-473, 1991.   DOI
18 Mortensen, H.F., Heuer, O.E., Lorenzen, N., Otte, L. and Olesen, N.J.: Isolation of viral haemorrhagic septicaemia virus (VHSV) from wild marine fish species in the Baltic Sea, Kattegat, Skagerrak and the North Sea. Virus Res., 63: 95-106, 1999.   DOI   ScienceOn
19 Roitt, I.M, Brostoff, J. and Male D.K. Immunology. London, Mosby., 225. 1998
20 Schreiber, A., Luft, F.C. and Kettritz R.: Membrane proteinase 3 expression and ANCA-induced neutrophil activation. Kidney Int., 65: 2172-2183, 2004.   DOI
21 Smail, D.A. Viral haemorrhagic septicaemia. In: Fish disease and disorders, vol. 3, Viral, bacterial and fungal infections. P.T.K. Woo, D.W. Bruno (ed), CABI Publishing, New York, 123-147,1999.
22 Praveen, K., Evans, D.L. and Jaso-Friedmann L.: Evidence for the existence of granzyme-like serine proteinases in teleost cytotoxic cell. J Mol Evol., 58: 449-459, 2004.   DOI
23 Takano, R., Nishizawa, T., Arimoto M. and Muroga K. Isolation of viral haemorragic septicemia (VHSV) from wild Japanese flounder, Paralichthys olivaceus. Bull Eur Assoc Fish Pathol., 20: 186-193, 2000.
24 Thompson, C.B.: Apoptosis in the pathogenesis and treatment of disease. Science, 267: 1456-1462, 1995.   DOI   ScienceOn
25 Uehara, A., Muramoto, K. Takada, H. and Sugawara, S.: Neutrophil serine proteinases activate human nonepithelial cells to produce inflammatory cytokines through protease-activated receptor 2. J Immunol., 70: 5690-5696, 2003.
26 Dipanjan, C. and Lieberman J.: Death by a thousand cuts: granzyme pathways of programmed cell death. Annu Rev Immunol., 26: 389-420, 2008.   DOI   ScienceOn