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http://dx.doi.org/10.7847/jfp.2012.25.2.117

Pathogenicity of viral hemorrhagic septicemia virus (VHSV) isolated from olive flounder Paralichthys olivaceus to masu salmon Oncorhynchus masou  

Kim, Wi-Sik (The Fisheries Science Institute, Chonnam National University)
Kim, Jeong-Ho (Department of Marine Bioscience, Gangneung-Wonju National University)
Nishizawa, Toyohiko (Department of Aqualife Medicine, Chonnam National University)
Oh, Myung-Joo (Department of Aqualife Medicine, Chonnam National University)
Publication Information
Journal of fish pathology / v.25, no.2, 2012 , pp. 117-121 More about this Journal
Abstract
The pathogenicity of viral hemorrhagic septicemia virus (VHSV) from olive flounder Paralichthys olivaceus was investigated with masu salmon Oncorhynchus masou fry. The cumulative mortality of fish challenged with FYeosu05 isolate at $10^{6.5}$ $TCID_{50}$/fish was 60%. No mortality was observed in fish challenged with the isolates at $10^{5.5}$ $TCID_{50}$/fish and in mock-challenged fish. The affected fish showed darkening of the body, expanded abdomen, pale gills and enlarged spleen. VHSV from $10^{6.3}$ to $10^{7.8}$ $TCID_{50}$/g-tissue was re-isolated from the dead fish. These results suggest that the VHSV from olive flounder is pathogenic to masu salmon fry, although with low virulence.
Keywords
Viral hemorrhagic septicemia virus; Masu salmon; Pathogenicity;
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1 Benmansour, A., Basurco, B., Monnier, A.F., Vende, P., Winton, J.R. and de Kinkelin, P.: Sequence variation of the glycoprotein gene identifies three distinct lineages within field isolates of viral haemorrhagic septicaemia virus, a fish rhabdovirus. J. Gen. Virol., 78: 2837-2846, 1997.
2 Brunson, R., True, K. and Yancey, J.: VHS virus isolated at Makah National Fish Hatchery. Am. Fish Soc. Newsl., 17: 3-4, 1989.
3 Einer-Jensen, K., Ahrens, P., Forsberg, R. and Lorenzen, N.: Evolution of the fish rhabdovirus viral haemorrhagic septicaemia virus. J. Gen. Virol., 85: 1167-1179, 2004.   DOI
4 Elsayed, E., Faisal, M., Thomas, M., Whelan, G., Batts, W. and Winton, J.: Isolation of viral haemorrhagic septicaemia virus from muskellunge, Esox masquinongy (Mitchill), in Lake St Clair, Michigan, USA reveals a new sublineage of the North American genotype. J. Fish Dis., 29: 611-619, 2006.   DOI   ScienceOn
5 Gagne, N., MacKinnon, A.M., Boston, L., Souter, B., Cook- Versloot, M., Griffiths, S. and Olivier, G.: Isolation of viral haemorrhagic septicaemia virus from mummichog, stickleback, striped bass and brown trout in eastern Canada. J. Fish Dis., 30: 213-223, 2007.   DOI   ScienceOn
6 Hedrick, R.P., Batts, W.N., Yun, S., Traxler, G.S., Kaufman, J. and Winton, J.R.: Host and geographic range extensions of the North American strain of viral hemorrhagic septicemia virus. Dis. Aquat. Org., 55: 211-220, 2003.   DOI
7 Hopper, K.: The isolation of VHSV from Chinook salmon at Glenwood Springs, Orcas Island, Washington. Am. Fish Soc. Newsletter, 17: 1, 1989.
8 Isshiki, T., Nishizawa, T., Kobayashi, T., Nagano, T. and Miyazaki, T.: An outbreak of VHSV (viral hemorrhagic septicemia virus) infection in farmed Japanese flounder Paralichthys olivaceus in Japan. Dis. Aquat. Org., 47: 87-99, 2001.   DOI
9 Kim, W.S., Jung, S.J., Kim, J.O., Kim, D.W., Kim, J.H. and Oh, M.J.: Genetic positioning of Korean viral hemorrhagic septicemia virus (VHSV) from cultured and wild marine fishes. J. Fish Pathol., 24: 1-9, 2011a.   DOI   ScienceOn
10 Kim, W.S., Kim, S.R., Kim, D.W., Kim, J.O., Park, M.A., Kitamura, S.I., Kim, H.Y., Kim, D.H., Han, H.J., Jung, S.J. and Oh, M.J.: An outbreak of VHSV (viral hemorrhagic septicaemia virus) infection in farmed olive flounder Paralichthys olivaceus in Korea. Aquaculture, 296: 165-168, 2009.   DOI   ScienceOn
11 Kim, W.S., Nishizawa, T., Kim, J.H., Suebsing, R., Jung, S.J. and Oh, M.J.: Korean and Japanese isolates of viral hemorrhagic septicemia virus from olive flounder are pathogenic to rainbow trout fry. Fish Pathol., 46: 112-115, 2011b.   DOI
12 Meyers, T.R. and Winton, J.R.: Viral hemorrhagic septicemia virus in North America. Annu. Rev. Fish Dis., 5: 3-24, 1995.   DOI   ScienceOn
13 Mortensen, H.F., Heuer, O.E., Lorenzen, N., Otte, L. and Olesen, N.J.: Isolation of viral haemorrhagic septicaemia virus (VHSV) from wild marine fishspecies in the Baltic Sea, Kattegat, Skagerrak and the North Sea. Virus Res., 63: 95-106, 1999.   DOI   ScienceOn
14 Nishizawa, T., Iida, H., Takano, R., Isshiki, T., Nakajima, K. and Muroga, K.: Genetic relatedness among Japanese, American and European isolates of viral hemorrhagic septicemia virus (VHSV) based on partial G and P genes. Dis. Aquat. Org., 48: 143-148, 2002.   DOI
15 Stone, D.M., Way, K. and Dixon, P.F.: Nucleotide sequence of the glycoprotein gene of viral haemorrhagic septicaemia (VHS) viruses from different geographic areas: a link between VHS in farmed fish species and viruses isolated from North Sea cod (Gadus morhua L.). J. Gen. Virol., 78: 1319-1326, 1997.
16 Schlotfeldt, H.J., Ahne, W., Jørgensen, P.E.V. and Glende, W.: Occurrence of viral haemorrhagic septicaemia in turbot (Scophthalmus maximus) - a natural out-break. Bull. Eur. Assoc. Fish Pathol., 11: 105-107, 1991.
17 Skall H.F., Slierendrecht W.J., King J.A. and Olesen N.J.: Experimental infection of rainbow trout Oncorhynchus mykiss with viral haemorrhagic septicaemia virus isolates from European marine and farmed fishes. Dis. Aquat. Org. 58: 99-110, 2004.   DOI
18 Smail, S.A.: Viral haemorrhagic septicaemia. In Woo, P.T.K. and et Bruno, D.W., editors. Fish diseases and disorders, Vol. 3: Viral, bacterial and fungal infections. CAB International, New York, USA. pp.123-146, 1999.
19 Takano, R., Nishizawa, T., Arimoto, M. and Muroga, K.: Isolation of viral haemorrhagic septicaemia virus (VHSV) from wild Japanese flounder, Paralichthys olivaceus. Bull. Eur. Assoc. Fish Pathol., 20: 186-192, 2000.
20 Trdo, N., Benmansour, A., Calisher, C., Dietzgen, R.G., Fang, R.X., Jackson, A.O., Kurath, G., Nadin-Davis, S., Tesh, R.B. and Walker, P.J.: Family Rhabdoviridae. In Fauquet, C.M., Mayo, M.A., Maniloff, J., Desselberger, U. and Ball, L.A., editors. Virus taxonomy: Eighth report of the international committee on taxonomy of viruses. Elsevier/Academic Press, London, United Kingdom. pp. 623-644, 2005.
21 Watanabe, L., Pakingking, R.J., Iida, H., Nishizawa, T., Iida, Y., Arimoto, M. and Muroga, K.: Isolation of aquabirnavirus and viral hemorrhagic septicemia virus (VHSV) from wild marine fishes. Fish Pathol., 37: 189-191, 2002.   DOI
22 Wolf, K.: Viral hemorrhagic septicemia. In Wolf, K., editor. Fish viruses and fish viral diseases. Cornell University Press, Ithaca, New York, USA. pp. 217-249, 1988.
23 Yasutake, W.T.: Fish viral diseases: clinical, histopathological, and comparative aspects. In: Ribelin, W.E., Migaki, G. (eds) The pathology of fishes. The University of Wisconsin Press, Madison, pp. 247-271, 1975.
24 Yasutake, W.T.: Comparative histopathology of epizootic salmonid virus diseases. In: Snieszko, S.F. (ed) A symposium on diseases of fishes and shellfishes. American Fisheries Society, Washington DC, pp. 341-350, 1970.