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http://dx.doi.org/10.7847/jfp.2011.24.3.189

Efficacy of a vaccine against Streptococcus parauberis infection in starry flounder Platichthys stellatus Pallas  

Lee, Deok-Chan (Southeast Sea Fisheries Research Institute, NFRDI)
Lee, Jae-Il (Gyeongsangbuk-do Fisheries Technology Center)
Kim, Do-Hyung (Department of Aquatic Life Medicine, Chonnam National University)
Cho, Mi-Young (Aquatic Animal Diseases Control Center, NFRDI)
Kim, Jin-Woo (Aquatic Animal Diseases Control Center, NFRDI)
Publication Information
Journal of fish pathology / v.24, no.3, 2011 , pp. 189-195 More about this Journal
Abstract
Starry flounder, which are recently increasingly cultured in Korea, are known to highly vulnerable to Streptococcus parauberis infection. Five groups of starry flounder (n=30 for each group) were vaccinated with S. parauberis formalin-killed whole cells by intraperitoneal injection at a final concentration of 0, 0.01, 0.1, 1 and 10 mg $fish^{-1}$. Specific antibody production of 1 and 10 mg $fish^{-1}$ administered groups significantly increased at four weeks post immunization. All vaccinated groups showed higher survival rates than a control group when five groups of fish were challenged with S. parauberis at a dose of $1.14{\times}10^4$ cfu $fish^{-1}$ and $1.14{\times}10^2$ cfu $fish^{-1}$, respectively. In particular, 0.1 or higher concentrations of formalin killed bacterial cells are able to confer the fish high protection against S. parauberis infection.
Keywords
Streptococcus parauberis; Platichthys stellatus; Vaccine; Agglutination antibody titer; Challenge test;
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