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http://dx.doi.org/10.7847/jfp.2011.24.1.011

Pathophysiology of olive flounder Paralichthys olivaceus suffering from emaciation  

Kim, Yi-Kyung (Department of Aquatic Life Medicine, Pukyong National University)
Jeong, Joon-Bum (School of Marine Biomedical Sciences, Jeju National University)
Lee, Mu-Kun (Department of Aquatic Life Medicine, Pukyong National University)
Park, Soo-Il (Department of Aquatic Life Medicine, Pukyong National University)
Park, Myeong-Ae (Pathology Division, National Fisheries Research and Development Institute)
Choe, Mi-Kyung (Jeju Branch, Korea Fisheries Resources Agency)
Yeo, In-Kyu (School of Marine Biomedical Sciences, Jeju National University)
Publication Information
Journal of fish pathology / v.24, no.1, 2011 , pp. 11-18 More about this Journal
Abstract
This study was aimed to investigate the pathophysiological changes of olive flounder, Paralichthys olivaceus suffering from emaciation. A plasma osmolality was higher in the emaciated and control flounders than that of normal teleost, suggesting osmoregulatory failure in both of them. Also, the control in the same stock with emaciated flounder seem to be classified into a primary degree of emaciation. According to microscopic observations, the inflammatory responses were observed in the submucosal layer of anterior intestine, although the some of mucosal intestinal epithelium still remained. It was suggested that the pathological changes of the anterior part give rise to malabsorption of nutrients through the mucosa. In the posterior intestine and rectum, the mucosal epithelium were almostly sloughed off and severe inflammatory responses were observed in the submucosa. Immunoreaction for NKCC was not detected in the mucosal epithelial cells in intestine because of sloughing of epithelium. These changes would lead to functional disorder in the intestine, such as malabsorption of nutrients and osmoregulatory failure. Also important is to investigate the recovery phase.
Keywords
Olive flounder; Paralichthys olivaceus; Emaciation; Osmoregulatory failure; $Na^+/K^+/2Cl^-$ (NKCC); cotransporter; Intestine;
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1 Starremans, P.G., Kersten, F.F., Knoers, N.V., van den Heuvel, L.P. and Bindels, R.J.: Mutations in the human Na-K-2Cl cotransporter (NKCC2) identified in Bartter syndrome type I consistently result in nonfunctional transporters. J. Am. Soc. Nephrol., 14:1419-1426, 2003.   DOI   ScienceOn
2 Tin Tun, Ogawa, K. and Wakabayashi, H.: Pathological changes induced by three myxosporeans in the intestine of cultured tiger puffer, Takifugu rubripes (Temminck and Schlegel). J. Fish Dis., 25:63-72, 2002.   DOI   ScienceOn
3 Yanagida, T., Nomura, Y., Kimura, T., Fukuda, Y., Yokoyama, H. and Ogawa, K.: Molecular and morphological redescriptions of enteric myxozoans, Enteromyxum leei (formerly Myxidium sp. TP) and Enteromyxum fugu comb. n. (syn. Myxidium fugu) from cultured tiger puffer. Fish Pathol., 39:137-143, 2004.   DOI
4 Veillette, P.A., White, R.J., Specker, J.L.: Changes in intestinal fluid transport in Atlantic salmon (Salmo salar L) during parr-smolt transformation. Fish Physiol. Biochem. 12:193-202, 1993.   DOI   ScienceOn
5 Knepper, M.A. and Brooks, H.L.: Regulation of the sodium transporter NHE3, NKCC2 and NCC in the kidney. Curr. Opin. Nephrol. Hypertens., 10:655-659, 2001.   DOI   ScienceOn
6 Loretz, C.A.: Electrophysiology of ion transport in teleost intestinal cells. In: Cellular and Molecular Approaches to Fish Ionic Regulation, pp.25-56, Academic, New York, 1995.
7 Marshall, W.S. and Grosell, M.: Ion transport, osmoregulation, and acid-base balance, In The physiology of fishes, pp.179-230, CRC Press, Boca Raton, 2006.
8 Mount, D.B., Delpire, E., Gamba, G., Hall, A.E., Poch, E., Hoover, R.S. and Herbert, S.C.: The electroneutral cation-chloride cotransporter. J. Exp. Biol., 201:2091-2102, 1998.
9 Ogawa, K. and Yokoyama, H.: Emaciation disease of cultured tiger puffer Takifugu rubripes. Bull. Natl. Res. Inst., Suppl., 5:65-70, 2001.
10 Smith, H.W.: The absorption and excretion of water and salts by marine teleosts. Am. J. Physiol. 93:480-505, 1930.
11 Hirano, T. and Mayer-Gostan, N.: Eel esophagus as an osmoregulatory organ. Proc. Natl. Acad. Sci., USA, 73:1348-1350, 1976.   DOI   ScienceOn
12 Hiroi, J., Yasumasu, S., McCormick, S.D., Hwang, P.P. and Toyoji, K.: Evidence for an apical Na-Cl cotransporter involved in ion uptake in a teleost fish. J. Exp. Biol. 211:2584-2599, 2008.   DOI   ScienceOn
13 Isenring, P., Jacoby, S.C., Chang, J. and Forbush, B.: Mutagenic mapping of the Na-K-Cl cotransporter for domains involved in ion transport and bumetanide binding. J. Gen. Physiol., 112:549-558, 1998.   DOI   ScienceOn
14 Evans, D.H.: Osmotic and ionic regulation, pp.315-341, CRC Press, Boca Raton, 1993.
15 Ishimatsu, A., Hayashi, M., Nakane, M. and Sameshima, M.: Pathophysiology of cultured tiger puffer Takifugu rubripes suffering from the Myxosporean emaciation disease. Fish Pathol., 42:211-217, 2007.   DOI
16 Kim, Y.K., Ideuchi, H., Watanabe, S., Park, S.I., Huh, M.D. and Kaneko, T.: Rectal water absorption in seawater-adapted Japanese eel Anguilla japonica. Comp. Biochem. Physiol. A Mol. Integr. Physiol., 151:533-541, 2008.   DOI   ScienceOn
17 Cutler, C.P. and Cramb, G.: Differential expression of absorptive cation-chloride-cotransporters in the European eel Anguilla anguilla. Comp. Biochem. Physiol. B. 149:63-73, 2008.   DOI   ScienceOn
18 Greger, R.: Ion transporter mechanisms in thick ascending limb of Henle's loop of mammalian nephron. Physiol. Rev. 65:760-797, 1985.   DOI
19 Hass, M. and Forbush, B.III.: The Na-K-Cl cotranspoters of secretory epithelia. Annu. Rev. Physiol., 62:515-534, 2000.   DOI   ScienceOn
20 Hass, M. and Forbush, B.III.: The Na-K-Cl cotranspoters. J. Bioenerg. Biomembr. 30:161-172, 1998.   DOI   ScienceOn
21 Ando, M., Mukuda T. and Kozaka, T.: Water metabolism in the eel acclimated to seawater:from mouth to intestine. Comp. Biochem. Physiol. B., 136: 621-633, 2003.
22 Warth, R., Bleich, M., Thiele, I.I., Lang, F. and Greger, R.: Regulation of the $Na^+2Cl^-K^+$cotransporter in in vitro perfused rectal gland tubules of squalus acanthias. Pflugers Arch., 436:521-528, 1998.   DOI   ScienceOn
23 Aoki, M., Kaneko, T., Katoh, F., Hasegawa, S., Tsutsui, N. and Aida, K.: Intestinal water absorption through aquaporin 1 expressed in the apical membrane of mucosal epithelial cells in seawater-adapted Japanese eel. J. Exp. Biol., 206:3495-3505, 2003.   DOI   ScienceOn
24 Bentley, P.J.: Endocrines and osmoregulation, In Zoophysiology, vol.39, Springer, Berlin, 2002.
25 Collie, N.L. and Bern, H.A.: Changes in intestinal fluid transport associated with smoltification and seawater adaptation in coho salmon, Oncorhynchuskisutch(Walbaum)., J. Fish Biol. 21:337-348, 1982.   DOI