Browse > Article

Effects of garlic Allium sativum extract immersion on the immune responses of olive flounder Paralichthys olivaceus prechallenged with pathogenic bacteria  

Woo, Sung-Ho (Department of Aquatic Life Medicine, Pukyong National University)
Lee, Jun-Hee (Department of Aquatic Life Medicine, Pukyong National University)
Kim, Yi-Kyung (Department of Aquatic Life Medicine, Pukyong National University)
Cho, Mi-Young (Aquatic life Disease Control Division, National Fisheries Research and Development Institute)
Jung, Sung-Hee (Pathology Division, National Fisheries Research and Development Institute)
Kim, Jin-Woo (Aquatic life Disease Control Division, National Fisheries Research and Development Institute)
Park, Soo-Il (Department of Aquatic Life Medicine, Pukyong National University)
Publication Information
Journal of fish pathology / v.23, no.2, 2010 , pp. 199-209 More about this Journal
Abstract
This study was aimed to investigate the effects in different immersion doses of garlic, Allium sativum, juice to modulate on the nonspecific immune responses of the olive flounder, Paralichthys olivaceus, artificially prechallenged with Streptococcus iniae BS10 and Edwardsiella tarda KE-1, respectively. The nonspecific immune responses of the tested fish were assessed in term of skin mucus lysozyme activity, the change of bacterial cell counts in organs, the number of lymphocytes and neutrophils in blood, and SOD activity. Almost groups of the prechallenged with either S. iniae BS10 or E. tarda KE-1 fish which had been immersed in garlic juice showed the enhanced skin mucus lysozyme activity, the number of lymphocytes and neutrophils in blood, and SOD activity in the kidney but the decreased the number of bacterial cell in surveyed organs. RPS in the group immersed in 0.25 g/L of garlic juice was much higher than in other immersed test groups. These results suggested that the garlic juice immersion can be effective on enhancement of the nonspecific immune responses and the protective ability of olive flounder to the artificial challenge with S. iniae BS10 and E. tarda KE-1.
Keywords
Garlic; Allium sativum; Olive flounder; Paralichthys olivaceus; Immune response; Immunostimulant;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
연도 인용수 순위
1 Campa-Cordova, A.I., Hernandez-Saavedra, N.Y., De Philippis, R. and Ascencio F.: Generation of superoxide anion and SOD activity in haemocytes and muscle of American white shrimp (Litopenaeus vannamei) as a response to $\beta$-glucan and sulphated polysaccharide. Fish Shellfish Immunol., 12: 353-366, 2002.   DOI   ScienceOn
2 Jorgensen, J.B., Sharp, G.J.E., Secombes, C.J. and Robertsen, B.: Effect of a yeast-cell wall glucan on the bactericidal activity of rainbow trout macrophages. Fish Shellfish Immunol., 3:267-277, 1993b.   DOI   ScienceOn
3 Selaraj, V., Sampath, K. and Sekar, C.: Administration of yeast glucan enhances survival and some non-specific and specific immune parameters in carp (Cyprinus carpio) infected with Aeromonas hydrophila. Fish Shellfish Immunol., 19:293-306, 2005.   DOI   ScienceOn
4 Siwicki, A.K., Anderson, D.P. and Dixon, O.W.: In vitro immunostimulation of rainbow trout Oncohynchus mykiss spleen cells with lavamisole. Dev. Comp. Immunol., 14:231-237, 1990.   DOI   ScienceOn
5 이준희, 우승호, 엄용환, 황분옥, 권문경, 방종득, 박수일:마늘, Allium sativum이 넙치, Paralichthys olivaceus의 면역반응에 미치는 영향. 한국어병학회지, 23:69-83, 2010.   과학기술학회마을
6 Yano, T., Mangindaan, R.E.P. and Matsuyama, H.: Enhancement of the resistance of carp Cyprinus carpio to experimental Edwardsiella tarda infection, by some $\beta$-1,3 glucans. Nippon Suisan Gakkaishi, 55:1815-1819, 1991.
7 Kawakami, H., Hiratsuka, M. and Dosako, S.: Effects of iron- saturated lactoferrin on iron absorption. Agri. Biol. Chem., 52:903-908, 1998.
8 Song, Y.L. and Hsieh, Y.T.: Immunostimulation of tiger shrimp (Penaeus monodon) hemocytes for generation of microbicidal substances: analysis of reactive oxygen species. Dev. Comp. Immunol., 18:201-209, 1994.   DOI   ScienceOn
9 Villamil, L., Figueras, A. and Novoa, B.: Immunomodulatory effects of nisin in turbot (Scophthalmus maximus L.). Fish Shellfish Immunol., 14:157-69, 2003.   DOI   ScienceOn
10 Ordas, M.C., Novoa, B. and Figueras, A.: Modulation of the chemiluminescence response of mediterranean mussel (Mytilus galloprovincialis) haemocytes. Fish Shellfish Immunol., 10:611-622, 2000.   DOI   ScienceOn
11 Kitao, T., Yoshida, T., Anderson, D.P., Dixon, O.W. and Blanch, A.:Immuno-stimulation of antibodyproducing cells and humoral antibody to fish bacterins by a biological response modifier. J. Fish Biol., 31:87-91, 1987.   DOI
12 Schwartz, I.F., Hershkovitz, R., Iaina, A., Gnessin, E., Wollman, Y., Chenichowski, T., Blum, M., Levo, Y. and Schwartz, D.: Garlic attenuates nitric oxide production in rat cardiac myocytes through inhibition of inducible nitric oxide synthase and the arginine transporter CAT-2 (cationic amino acid transporter-2). Clin. Sci., 102:487-493, 2002.   DOI   ScienceOn
13 Raa, J., Torstad, G., Engstad, R. and Robertsen, B.: The use of immunostimulants to increase resistance of aquatic organisms to microbial infections. (in) M. Sharif, R. P. Subasighe and Arthur, J. R. (eds.), Dis. Asian A. Vol. 1. Fish Health Section, Asian Fish. Soc. Manila, Philippines, 39-50, 1992.
14 Reeve, V.E., Bosnic, M., Rozinova, E. and Boehm-Wilcox, C.: A garlic extract protects from ultraviolet B (280-320 nm) radiation-induced suppression of contact hypersensitivity. Photochem. Photobiol., 58:813-817, 1993.   DOI   ScienceOn
15 Sakai, M.: Current research status of fish immunostimulants. Aquaculture, 172:63-92, 1999.   DOI   ScienceOn
16 Kim, K.H., Hwang, Y.J. and Bai, S.C.: Enhancement of chemiluminescent response of phagocytic cells from juvenile Rockfish, Sebastes schlegeli, by oral administration of levamisole. J. Fish Sci. Tech., 1:42-47, 1998.
17 Jorgensen, J.B., Lunde, H. and Robertsen, B.: Peritoneal and head kidney cell response to intraperitoneally injected yeast glucan in Atlantic salmon, Salmo salar L. J. Fish Dis., 16:313-325, 1993a.   DOI   ScienceOn
18 Fu, Y.W., Hou, W.Y., Yeh, S.T, Li, C.H. and Chen. J.C.: The immunostimulatory effects of hot-water extract of Gelidium amansii via immersion, injection and dietary administrations on white shrimp Litopenaeus vannamei and its resistance against Vibrio alginolyticus, Fish Shellfish Immunol., 25:673-685, 2007.
19 Grondel, J.L., Nouws, J.F.M., DeJong M., Schutte, A.R., and Driessens, F.: Pharmacokinetics and tissue distribution of oxytetracycline in carp, Cyprinus carpio L., following different routes of administration. J. Fish Dis., 10:153-163, 1987.   DOI
20 Jeney, G. and Anderson, D.P.: Enhanced immune response and protection in rainbow trout to Aeromonas salmonicida bacterin following prior immersion in imunostimulants. Fish Shellfish Immunol., 3:51-8. 1993.   DOI   ScienceOn
21 Engstad, R.E., Robertson, B. and Frivold, E.: Yeast glucan induce increase in activity of lysozyme and complement-mediated haemolytic activity in Atlantic salmon blood. Fish Shellfish Immunol., 2:287-297, 1992.   DOI
22 Aoki, T.: Present and future problems concerning the development of drug resistance in Aquaculture, In Chemotherapy in Aquaculture : From Theory to Reality, Office International Des Epizooties. Paris, France, 254-262, 1992.
23 Arora, D.S. and Kaur, J.: Antimicrobial activity of spices. Intern. J. Antimicrob. Agents, 12:257-262, 1999.   DOI   ScienceOn
24 Benkeblia, N.: Antimicrobial activity of essential oil extracts of various onions (Allium cepa) and garlic (Allium sativum). Lebensm.-Wiss. + Technol., 37:263-268, 2004.   DOI   ScienceOn
25 Solem, S.T., Jorgensen, J.B. and Robertsen, B.: Stimulation of respiratory burst and phagocytic activity in Atlantic salmon Salmo salar L. macrophages by lipopolysaccharide. Fish Shellfish Immunol., 5:475-491, 1995.   DOI   ScienceOn
26 Ankri, S., Miron, T. and Rabinkov, M.: Allicin from garlic strongly inhibits cysteine proteinases and cytopathic effects of Entamoeba histolytica. Antimicrob. Agents Chemother., 41:2286-2288, 1997.
27 이덕찬: Edwardsiella tarda extracellular products (ECPs)의 인위 투여에 따른 넙치, Paralichthys olivaceus의 생체 반응. 한국어병학회지, 18:215-225, 2005.
28 Yeh, S.P., Chang, C.A., Chang, C.Y., Liu, C.H. and Cheng, W.: Dietary sodium alginate administration affects fingerling growth and resistance to Streptococcus sp. and iridovirus, and juvenile non-specific immune responses of the orange-spotted grouper, Epinephelus coioides. Fish Shellfish Immunol., 25:19-27, 2008.   DOI   ScienceOn
29 Zhang, Z., Swain, T., Bogwald, J., Dalmo, R.A. and Kumari., J.: Bath immunostimulation of rainbow trout (Oncorhynchus mykiss) fry induces enhancement of inflammatory cytokine transcripts, while repeated bath induce no changes. Fish Shellfish Immunol., 26:677-684, 2009.   DOI   ScienceOn
30 우승호: 해수 양식 어류에서 분리된 연쇄상구균의 종류와 병원성. 한국어병학회지, 19:17-33, 2006.
31 Murray, C.K. and Fletcher, T.C.: The immunohistochemical location of lysozyme in plaice (Pleuronectes platessa L.) tissue. J. Fish. Biol., 9: 329-334, 1976.   DOI
32 Kodama, H., Mukamoto, N., Baba, T. and Mule, D.M.: Macrophage-colony stimulating activity in rainbow trout Oncorhynchus mykiss serum. (in) J.S. Stolen and T.C. Fletcher(eds), Modulators of fish Immune Responses, Vol. 1, SOS Publication, Fair Haven, NJ, 59-66, 1994.
33 Kyo, E., Naoto Uda, N., Kasuga, S. and Itakura, Y.: Immunomodulatory Effects of Aged Garlic Extract. J. Nutr., 131:1075S-9S. 2001.   DOI
34 Miles, A.A. and Misra, S.S.: The estimate of the bactericidal power of the blood. J. Hyg., 38:873-885, 1938.
35 O'Gara, E.A., Hill, D.J. and Maslin, D.J.: Activities of garlic oil, garlic powder, and their diallyl constituents against Helicobacter pylori. Appl. Environ. Microbiol., 66:2269-2273, 2001.
36 Kajita, Y., Sakai, M., Kobayashi, M. and Kawaushi, H.: Enhancement of non-specific cytotoxic activity of leucocytes in rainbow trout Oncorhynchus mykiss injected with growth hormone. Fish Shellfish Immunol., 2:155-157, 1992.   DOI