Browse > Article

Survey of Perkinsus olseni infection in Manila clam, Ruditapes philippinarum in 2009 on the west and south coast of Korea using PCR technique  

Lee, Nam-Sil (Division of Pathology, National Fisheries Research and Development Institute)
Hwang, Jee-Youn (Division of Pathology, National Fisheries Research and Development Institute)
Choi, Dong-Lim (Division of Pathology, National Fisheries Research and Development Institute)
Park, Myoung-Ae (Division of Pathology, National Fisheries Research and Development Institute)
Publication Information
Journal of fish pathology / v.23, no.2, 2010 , pp. 145-153 More about this Journal
Abstract
Prevalence of a protozoan parasite Perkinsus olseni in Manila clam Ruditapes philippinarum was surveyed from July to December 2009 on the west and south coast of Korea. P. olseni infection was diagnosed using two primer sets, P.olseni NTS Forward/P.olseni NTS Reverse set and PolsITS-140F/PolsITS-600R set in polymerase chain reaction(PCR). The results using PolsITS-140F and PolsITS-600R primer set was retained up to 60% at all stations from July to December, except for Padori. Especially, Goheung showed 100% prevalence from October to December. The results about comparison of the 4 station's DNA sequences which were analyzed from PCR products(457bp) using PolsITS-140F and PolsITS-600R primer set, there were only 2base differences at Sunjedo.
Keywords
Perkinsus olseni; Manila clam; Ruditapes philippinarum; PCR;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Choi, K.S., Park K.I., Cho M. and Soudant P.: Diagnosis, Pathology, and Taxonomy of Perkinsus sp. Isolated from the Manila clam Ruditapes philippinarum in Korea. J. Aquaculture, 18:207-214, 2005.   과학기술학회마을
2 Uddin, M.J., Yang, H.S., Choi, K.S., Kim, H.J., Hong, J.S. and Cho, M.: Seasonal Changed in Perkinsus olseni Infection and Gametogenesis in Manil Clam, Ruditapes philippinarum, From Seonjaedo Island in Incheon, off the West Coast of Korea. J. World Aquaculture society, 41:93-101, 2010.   DOI   ScienceOn
3 Dungan, C.F. and Reece K.S.: In vitro propagatio of two Perkinsus spp. Parasites from Japanese Manila clams Venerupis philippinarum and Description of Pekinsus honshuensis n. sp. J. Eukaryot. Microbiol. 53: 316-326, 2006   DOI   ScienceOn
4 Park, K.I., Park, J.K., Lee, J. and Choi, K.S.: Use of molecular markers for species identification of Korean Perkinsus sp. isolated from Manilaclam Ruditapes philippinarum. Dis. Aquat. Org., 66:255-263, 2005.   DOI
5 Park, K.-I. and Choi, K.-S.: Spatial distribution of the protozoan parasite Pekinsus sp. found in Manila clams, Ruditapes phillippinarum, in Korea. Aquaculture, 203:9-22, 2001.   DOI   ScienceOn
6 Moss, J.A., Xiao, J., Dungan, C.F. and Reece, K.S.: Description of Perkinsus beihaiensis n. sp., a new Perkinsus sp. parasite in oysters of southern China. J. Eukayot. Microbiol. 55:117-130, 2008.   DOI   ScienceOn
7 Murell, A., Kleenan, S.N., Barker, S.C. and Lester, R.J.G.: Synonymy of Perkinsus olseni Lester & Davis, 1981 and Perinsus atlanticus Azevedo, 1989 and an update on the phylogenetic position of the genus Perkinsus. Bull. Eur. Assoc. Fish Pathol., 22:258-265, 2002.
8 Noren, F., Moestrup O. and Rehnstam-Holm A.-S.:Parivilucifera infectans Noren et Moestrup gen. et sp. nov.(Perkinsozoa phylum nov.): a parasitic flagellate capable of killing toxic microalgae. Eur. J. Protistol., 35, 233-254,1999.   DOI   ScienceOn
9 OIE: Manual of Diagnostic Tests for Aquatic Animals, World organization for animal health, Paris, 2009.
10 Park, K.-I., Ngo, T.T.T., Choi, S.-D., Cho, M. and Choi, K.-S.: Occurrence of Perkinsus olseni in the Venus clam Protothaca jedoensis in Korean waters. J. Inv. Pathol. 93:81-87, 2006.   DOI   ScienceOn
11 Robledo J.A.F., Coss C.A. and Vasta G.R.: Characterization of the ribosomal RNA locus of Perkinsus atlanticusand development of a polymerase chain reaction-based diagnostic assay. J. Parastol., 86:972-978, 2000.   DOI
12 Villalba, A., Casas, S.M., Lopez, C. and Carballal, M.J.: Study of pekinsosis in the carpet shell clam Tapes decussatus in Galicia(NW Spain) II. Temporal pattern of disease dynamics and association with clam mortality. Dis. Aquat. Organ., 65:257-267, 2005.   DOI   ScienceOn
13 Hamaguchi, M., Suzuki, N., Usuk,i H. and Ishioka, H.: Perkinsus protozoan infection in shor-necked clam tapes(=Ruditapesphillippinarum) in Japan. Fish Pathol., 33:73-480, 1998.   DOI
14 Coss, C.A., Robledo, J.A.F., Ruita, G.M. and Vasta, G.R.: Description of Perkinsus andrewsi n. sp. isolated from the Balthic clam(Macoma balthica) by characterization of the ribosomal RNA locus, and development of a species-specific PCR-based diagnostic assay. J. Eukaryot. Microbiol., 48:52-61, 2001.   DOI   ScienceOn
15 Cremonte, F., Balseiro, P. and Figueras, A.: Occurrence of Perkinsus olseni(Protozoa:Apicomplexa) and other parasites in the venerid commercial clam Pitar rostrata from Uruguay, southwestern Atlantic coast. Dis. Aquat. Org., 64:85-90, 2005.   DOI
16 Goggin, D.L. and Lester, R.J.G.: Perkinsus, a protistan parasite of abalone in Australia: a review. Aust. J. Mar, Freshwater Res., 46:639-646, 1995.   DOI
17 La Peyre, M., Casas, S. and La Peyre, J.: Salinity effects on viability, metabolic activity and proliferation of three Perkinsus species. Dis. Aquat. Org.,71:59-74, 2006.   DOI   ScienceOn
18 Lester, R.J.G. and Davis, G.H.G.: A new Perkinsus species(Apicomplexa, Perkinsea) from the abalone, Halitis ruber. J. Invert. Pathol. 37:181-187, 1981.   DOI
19 Mackin, J.G., Owen, H.M. and Collier, A.: Preliminary note on the occurrence of a new protistan parasite, Dermocystidium marinum n. sp. in Crassostrea nirginica(Gmelin). Science, 111:328-329, 1950.   DOI
20 Marsh, A.G., Gauthier, J.D. and Vasta, G.R.: A seniquantitative PCR assay for assessing Perkinsus marinus infections in the eastern oyster, Crassostrea virginica. J. Parasitol., 81:577-583, 1995.   DOI   ScienceOn
21 McLayghlin, S.M., Tall, B.D., Shaheen, A., Elsayed, E.E. and Faisal, M:,Zoosporulation of a new Perkinsus species isolated from the gills of the softshell clam Mya arenaria. Parasite, 7:115-122, 2000.   DOI
22 Burreson, E.M. and Calvo, L.M.R.: Epizootiology of Perkinsus marunus disease of oysters in Chesapeake Bay, with emphasis on data since 1985. J. Shellfish Res., 15:17-34, 1996.
23 Moss, J.A.: Characterization of exotic pathogens associated with the suminoe oyster, Crassostrea ariakensis. Ph.D. Sissertaion. Virginia Institute of Marine Science. College of William and Mary, Gloucester Point, Virginia USA. p.230, 2007.
24 Azevedo, C.: Fine structure of Perkinsus atlanticus n. sp. (apicomplexa, Perkinsea) parasite of the clam Ruditapes decussatus from Portugal. J. Parasitol., 75:627-635, 1989.
25 Blackbourn, J., Bower, S.M. and Meyer, G.R.: Perkinsus qugwadi sp. nov.(incertace sedis), a pathogenic protozoan parasite of Japanese scallops, Patinopecten yessoensis, cultured in British Columbia, Canada. Can. J. Zool., 7:942-953, 1998.
26 Casas, S.M., Grau, A., Kimberly, S.R., Apalupakul, K., Azevedo, C. and Villalba A.: Perkinsus mediterraneus n. sp., a protistan parasite of the European flat oyster Ostrea edulis from the Balearic Islands, Mediterranean Sea, Dis. Aquat. Org., 58:231-234, 2004.   DOI   ScienceOn
27 Choi, K.S. and Park, K.I.: Review on the Protozoan Parasite Perkinsus olsei(Lester and Davis 1981) Infection in Asian Water. Coastal Environmental and Ecosystem Issues of the East China Sea, Eds., A. Ishimatsu and H.-J. Lie, pp.269-281. 2010.