Browse > Article

Effects of low salinity stresses on the physiology of disc abalone, Haliotis discus discus  

Jwa, Min-Seok (Faculty of Applied Marine Sciences, Jeju National University)
Kang, Kyung-pil (Faculty of Applied Marine Sciences, Jeju National University)
Choi, Mi-Kyung (Jeju Fisheries Research Institute, National Fisheries Research Institute (NFRDI))
Yeo, In-Kyu (Faculty of Applied Marine Sciences, Jeju National University)
Publication Information
Journal of fish pathology / v.22, no.3, 2009 , pp. 293-303 More about this Journal
Abstract
Effects of stress on the low salinity stress were examined in the pacific abalone Haliotis discus discus. Changes in survival rate, hemolymph count, antioxidant enzyme activities (catalase: CAT and superoxide dismutase: SOD), respiratory burst activity, phenoloxidase activity, lysozyme activity and expression of heat shock protein 70 (HSP70) mRNA were measured 0, 3, 6, 12, 24 or 48hours after low salinity treatment with 25, 30, 33 and 35 psu. Survival rates of pacific abalone were 100% at 33 and 35 psu, but 93 and 97% at 25 and 30 psu for 48 hours, respectively. Hemolymph counts decreased in the time elapsed-dependent way at all of the experimental groups. At low salinity, 25 and 30 psu, SOD and CAT activity increased compared to the experimental group of 33 psu. Moreover, respiratory burst activities of the pacific abalone seemed to have no effect on low salinity stress at any experimental group. However, phenoloxidase activity is an important component of the defence against pathogen that was decreased in a reduction of salinity dependent way. Lysozyme activity also immediately reduced at 25 psu experimental group for 48 h. The HSP70 mRNA was weakly expressed at 33 psu, but strongly detectable at 25 psu experimental group. The HSP 70 mRNA expression in gill increased in the time elapsed-dependent way at 25 psu experimental group and then recovered at 48 h. These results suggest that low salinity stress give rise to inhibitory action of immune system as a result of the decrease of phenoloxidase and lysozyme activity in the pacific abalone, especially.
Keywords
Pacific abalone; Low salinity stress; Antioxidant enzyme; Lysozyme; Phenoloxidase;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
연도 인용수 순위
1 Chen, H.C.: Recent innovations in cultivation of edible molluscs in Taiwan with special reference to the small abalone Haliotis diversicolor and the hard clam metretrix lusoria. Aquaculture. 39:11-27, 1984   DOI   ScienceOn
2 Han, S.J., Kim, B.R., Won, S.H. and Kim, J.W.: Effect of Urea on the exfoliation of juvenile abalone, Haliotis discus reeve. J. Aquaculture, 16(4):223-228, 2003.(in korean)   과학기술학회마을   ScienceOn
3 Moon, J.H. and Pang, I.C.: Inflowing of Yangtze coastal water in summer and its cause. Bull. Mar. Res. Inst. ChejuNat'l. Univ., 27:29-47, 2003
4 Yang, H.S., Park, K.L., Hong, C.H. and Choi, K.S.: Effects of Salinity Stress on the Composition of Free Amino Acids of the Pacific abalone Haliotis discus discus. Kor. J. Aquaculture, 21:218-225, 2008   과학기술학회마을   ScienceOn
5 Marklund S. and Marklund, G.: Involvement of the superoxide anion radical in the antioxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur. J. Biochem., 47:469-474, 1974   DOI   ScienceOn
6 Kim, T.H., Yang, M.H., Choe, M.G., Han, S.J. and Yeo, I.K.: Physiological studies on acute water-temperature stress of abalone (Haliotis discus hannai). Kor. J. aquaculture, 18:7-12, 2005
7 Brown, P.B., Willson, K.A., Wetzel II, J.E. and Hoene, B.: Increased densities result in reduced weight gain of crayfish Orconectes virilis. Journal of the World Aquaculture Society, 26:165-171, 1995   DOI   ScienceOn
8 Shin, Y.K., Moon, T.S. and Wi, C.H.: Effects of the dissolved Oxygen concentration on the physiology of the manila clam, Tegillarca granosa(Linnaeus). J. Korean Fish. Soc., 35(5):485-489, 2002   DOI
9 Cho, K.S., Min, E.Y. and Kang, J.C.: Changes of haematological constituents in the nile tilapia, Oreochromis niloticus to HCB and PCBs. J. Kor. Fish. Soc., 35:110-114, 2002
10 Goldberg, B. and Stern, A.: The role of the superoxide anion as a toxic species in the erythrocyte. Arch. Biochem. Biophys., 178:218- 225, 1977   DOI   ScienceOn
11 Simon, R.H., Scoggin, C.H. and Patterson, D.: Hydrogen peroxide causes the fatal injury to human fibroblasts exposed to oxygen radicals. J. Biol. Chem., 256:7181-7186, 1981
12 제주특별자치도., 해양수산현황, pp.78-79, 1996
13 Chance, B., Sice, H. and Boveris, A.: Hydroperoxide metabolism in mammalian organs. Physiol, Rev., 59: 527-605, 1979   ScienceOn
14 Vijayan, M.M. and Moon, T.W.: The stress response and plasma disappearance of corticostreiod and glucose in a marine teleost sea raven. Can. J. Zool., 72:379-386, 1994   DOI   ScienceOn
15 Johnson J.K., Rocheleau, T.A., Hillyer, J.F., Chen, C.C., Li, J. and Christensen, B.M.: A potential role for phenylalanine hydroxylase in mosquito immune responses. Inspect. Biochem. Mol. Biol. 33:345-354, 2003   DOI   ScienceOn
16 Nelson D.P. and Kiesow, L.A.: Enthalpy of decomposition of hydrogen peroxide by catalase at 25$^{\circ}C$ (with molar extinction coefficients of $H_2O_2$ solutions in the UV). Anal Biochem., 49:474-478, 1972   DOI   ScienceOn
17 Jung, H.T., Kim, Y.S. and Choi, S.D.: The change of total weight and food consumption of abalone, Haliotis discus hannai under a vibration and noise. j. Environmental Sciences, 13(6):581-589, 2004.(in Korean)   과학기술학회마을   DOI   ScienceOn
18 Mgaya, Y.D. and Mereer, J.P.: The effects of size grading and stocking density on growth performance of juvenile abalone, Haliotis tuberculata Linnaeus. Aquaculture, 136:297-312, 1995   DOI   ScienceOn
19 Holliday, J.E., Allan, G.L. and Nell, J.A.: Effects of stocking density for nursery culture of Sydney rock oysters (Saccostrea commercialis). Aquaculture, 96:7-16, 1993   DOI   ScienceOn
20 Moody, C.S. and Hassan, H.M.: Mutagenicity of oxygen free radicals. Proc. Natl. Acad. Sci., 79:2855-2859, 1982   DOI   ScienceOn
21 Ferraris, M., Radice, S., Catalani, P., Francolini, M., Marabini, L. and Chiesara, E.: Early oxidative damage in primary cultured trout hepatocytes: a time course study. Aquatic Toxicology, 59:283-296, 2002   DOI   ScienceOn
22 Siwicki, A.K. Anderson, D.P. and Rumsey, G.L.: Dietary intake of immunostimulants by rainbow trout affects non-specific immunity and protection against furunculosis. Vet. Immunol. Immunopathol., 41:125-139, 1994   DOI   ScienceOn
23 wendel, A. and Feuerstein, S.: Drug-induced lipid peroxidation in mice-1. Modulation by monoxygenase activity, glutathione and selenium status. Biochem. Pharmacol., 30:2513-2520, 1981   DOI   ScienceOn
24 Drew, B., Miller, D., Toop, T. and Hanna, P.: Identification of experssed HSP's in black abalone (Haliotis rubra leach)during heat and salinity stresses. J. Shellfish. Research, 20(2):695-703, 2001   ScienceOn
25 Forman, H.J. and Fridovich, I.: Superoxide dismutase: A comparison of rate constant. Arch. Biochem. Biophys., 158:396-400, 1973   DOI   ScienceOn
26 Chen, J.C. and Chen, W.C.: Salinity tolerance of Haliotis diversicolor supertexta at different salinity and temperature levels. Aquaculture, 181:191-203, 2000   DOI   ScienceOn