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Pathological changes of the heart of olive flounder Paralichthys olivaceus in experimental Streptococcus parauberis infection  

Kim, Jin-Woo (Aquatic Animal Disease Control Center, National Fisheries Research and Development Institute)
Cho, Mi-Young (Aquatic Animal Disease Control Center, National Fisheries Research and Development Institute)
Won, Kyoung-Mi (Aquatic Animal Disease Control Center, National Fisheries Research and Development Institute)
Kim, Byoung-Gwan (Aquatic Animal Disease Control Center, National Fisheries Research and Development Institute)
Choi, Hee-Jung (Pathology Division, National Fisheries Research and Development Institute)
Han, Myoung-Chul (Seaworld Team, Hanwha 63 City)
Park, Myoung-Ae (Pathology Division, National Fisheries Research and Development Institute)
Publication Information
Journal of fish pathology / v.22, no.3, 2009 , pp. 253-262 More about this Journal
Abstract
We conducted bacteriological and histopathological analysis for olive flounder Paralichthys olivaceus after experimental infection with Streptococcus parauberis (FP2284) isolated from diseased olive flounder under different stress conditions. Experimental challenge was performed in healthy flounder (40.4 g in average body weight) by intraperitoneal (i.p.) injection with $2{\times}10^{8}$ CFU/fish under normal (no stress) or netting (for 2 min, twice/day) stress condition. The cumulative mortalities of no-stress and netting stress group were 70% and 95%, respectively. The most prevalent changes observed in experimentally infected flounder were darkness of skin and inflammation of the heart. Severe pericarditis, myocarditis and fibrosis were observed in the heart of the affected flounder. The results of viable counts showed the number of bacteria of the heart tissue was maintained over the $10^{4}$ CFU $g^{-1}$ heart for 3 weeks after inoculation. Histological lesions of the heart was more extensive and gradual decrease in bacterial numbers of heart tissue was delayed under stress condition.
Keywords
Streptococcus parauberis; Histological changes; Heart; Pericarditis; Olive flounder;
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Times Cited By KSCI : 8  (Citation Analysis)
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