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Phenotypic characteristics and antimicrobial susceptibilities of motile aeromonads isolated from freshwater fish in Korea and Japan  

Han, Hyun-Ja (Graduate school of Marine Science and Technology, Tokyo University of Marine Science and Technology, Konan, 4-5-7, Minato)
Govindasami, Vivekanandhan (Graduate school of Marine Science and Technology, Tokyo University of Marine Science and Technology, Konan, 4-5-7, Minato)
Hirono, Ikuo (Graduate school of Marine Science and Technology, Tokyo University of Marine Science and Technology, Konan, 4-5-7, Minato)
Aoki, Takahashi (Graduate school of Marine Science and Technology, Tokyo University of Marine Science and Technology, Konan, 4-5-7, Minato)
Publication Information
Journal of fish pathology / v.20, no.3, 2007 , pp. 249-255 More about this Journal
Abstract
We investigated the phenotypic characteristics by using API20E, APIZYM and determined minimum inhibitory concentrations (MICs) of 7 antibiotics in motile aeromonads isolated from freshwater fishes in Korea and Japan, and 4 American Type Culture Collection (ATCC) strains. All isolates (n=7) were identified as motile Aeromonas species according to API20E test. Lysine decarboxylase activity and acid production from 4 different carbohydrates including mannitol, rhamnose, amygdalin and arabinose were observed in various strains. In enzymatic activities by APIZYM, all isolates showed negative reactions in valine and cystine arylamidases, α-chymotrypsin, α-galactosidase, β-glucuronidase, α-glucosidase, α-mannosidase and α-fucosidase. Although the intensities of each enzymatic activity were diverse in alkaline phosphatase, esterase-lipase, leucine arylamidase, β-galactosidase and N-acetyl-β-glucosaminidase, all isolates showed positive reactions. All isolates were resistant to ampicillin sodium (MIC>100㎍/ml), but sensitive to chloramphenicol (MIC≤1.6㎍/ml). However, recently isolated strains (AC9804, AC0202 and GMA0361) were commonly resistant to tetracycline (MIC=50㎍/ml). Furthermore, AC9804 was resistant to oxolinic acid (MIC=12.5㎍/ml). GMA0361 was resistant to kanamycin sulfate (MIC>100㎍/ml) and streptomycin sulfate (MIC>100 ㎍/ml).
Keywords
Motile aeromonads; Freshwater fish; APIZYM; MIC;
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