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Innate immune responses of common carp, Cyprinus carpio L. against antiviral activity inducers  

Cho, Mi-Young (Pathology Team, National Fisheries Research & Development Institute)
Kim, Su-Mi (Department of Aquatic Life Medicine, Pukyong National University)
Kim, Eun-Jeon (Tongyeong branch office, National Fisheries Products Quality Inspection Service)
Shon, Sang-Gyu (Pathology Team, National Fisheries Research & Development Institute)
Kim, Jin-Woo (Pathology Team, National Fisheries Research & Development Institute)
Park, Soo-Il (Department of Aquatic Life Medicine, Pukyong National University)
Publication Information
Journal of fish pathology / v.20, no.2, 2007 , pp. 189-200 More about this Journal
Abstract
To investigate the innate immune response involved in early stage of anti-viral defence, carps were injected with UV-inactivated spring viraemia of carp virus (SVCV), poly inosinic:cytidylic acid (Poly I:C) and concanavalin A (Con A), respectively and examined lysozyme activity, serum complement activity and chemiluminescent (CL) response of leucocytes isolated from head kidney at 3 days post-injection. There was no significant difference in plasma lysozyme activities among all experimental groups. However, lysozyme activities of head kidney in the groups injected with antiviral activity inducers were significantly higher than those of the control injected with physiological saline. Bactericidal activities of serum of the groups injected with antiviral activity inducers were not significantly different from control group. However, the CL responses were significantly higher at lower dose of Poly I:C and Con A, whilst dose-dependent increase was shown in UV-inactivated SVCV-injected group. In the challenge test with 1×104 TCID50/fish of SVCV at 4 days post-injection, UV-inactivated SVCV- and Poly I:C-injected groups showed higher relative percent survival (RPS) than Con A-injected group. Furthermore, strong protection was observed in the group injected higher dose of Poly I:C although showed lower activities in lysozyme and CL response. These results suggested that Poly I:C might stimulate other factors belonging to non-specific immune system have induced protective immunity against the SVCV challenged.
Keywords
Innate immune response; Antiviral activity inducers; Carp; SVC; Poly I:C; Con A;
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