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Comparative study of Photobacterium damselae subsp. damselae and Vibrios on pathogenicity in vitro  

Kwon, Mun-Gyeong (Pathology Team, National Fisheries Research and Development Institute)
Kim, Myoung-Sug (Pathology Team, National Fisheries Research and Development Institute)
Cho, Byoung-Youl (Ulsan Regional Maritime Affairs and Fisheries office)
Kim, Jin-Woo (Pathology Team, National Fisheries Research and Development Institute)
Park, Soo-Il (Department of Aquatic Life Medicine, Pukyong National University)
Publication Information
Journal of fish pathology / v.20, no.1, 2007 , pp. 71-81 More about this Journal
Abstract
Photobacterium damselae subsp. damselae and 4 Vibrio spp.(V. anguillarum, V. splendidus, V. harveyi and V. ordalii) were isolated from the diseased olive flounders, Paralichthys olivaceus. The isolates were tested on the pathogenicity in vitro. The properties of extracellular products(ECPs) were investigated with enzymatic activities, hemolytic activities toward the sheep and olive flounder erythrocytes, and cytotoxicity activities on the cell-line. And potential signal transduction pathways of the bacterial internalization were detected by using signal transduction inhibitors. P. damselae was high in phospholipase activity, hemolytic activity to olive flounder erythrocytes and cytotoxicity activity. And P. damselae had diversified internalizing pathways as compared to isolated vibrios. Therefore, these activities may be related with pathogenicity of P. damselae.
Keywords
Photobacterium damselae; Vibrio; Pathogenicity; ECPs; Cytotoxicity;
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