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Characterization of Vibrio ponticus KJS1 Isolated from Larvae of Maroon clownfish, Premnas biaculeatus  

Kim, Jong-Su (Jeju Special Self-Governing Province Fisheries Resources Research Institute)
Rho, Sum (Faculty of Applied Marine Science, Cheju National University)
Kang, Bong-Jo (Jeju Special Self-Governing Province Fisheries Resources Research Institute)
Publication Information
Journal of fish pathology / v.20, no.1, 2007 , pp. 25-31 More about this Journal
Abstract
Vibriosis is one of the most important diseases affecting marin fish. In this study, a bacterium belonging to the Vibrio were isolated from maroon clownfish (Premnas biaculeatus) larvae (3～7 days after hatching). The bacterium was identified as Vibrio ponticus KJS1 by 16S rRNA gene sequence analysis. The result of biochemical test using the GN2 microplate (BioLog Inc, USA) showed that it has the ability to use 35 substrates including dextrin and sucrose was detected, could not degrade 52 substrates including α-cyclodextrin, and showed the weak ability to use 7 substrates including glycogen. It was sensitive to oxolinic acid, flumequine, ciprofloxacine, ofloxacin, norfloxacin, nalidixic acid, pefloxacin, and doxycycline hydrochloride.
Keywords
Maroon clownfish (Premnas biaculeatus); Vibrio ponticus; 16S rRNA gene;
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