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Effects of 17 β -estradiol, bisphenol A and genistein on the expression of the glutathione peroxidase gene of Philasterides dicentrarchii (Ciliophora: Scuticociliata)  

Lee, Eun-Hye (Department of Aquatic Life Medicine, Pukyong National University)
Kim, Sung-Mi (Department of Aquatic Life Medicine, Pukyong National University)
Nam, Yoon-Kwon (Department of Aquaculture, Pukyong National University)
Kim, Ki-Hong (Department of Aquatic Life Medicine, Pukyong National University)
Publication Information
Journal of fish pathology / v.19, no.3, 2006 , pp. 189-195 More about this Journal
Abstract
A subtracted cDNA library of a marine scuticociliate, Philasterides dicentrarchii, in response to 17β-estradiol exposure was constructed using suppression subtractive hybridization (SSH). As a result of SSH, 275 clones were isolated, and among them, only glutathione peroxidase (GPX) gene was isolated as an antioxidative enzyme responding to 17β-estradiol. The semi-quantitative reverse-transcriptase polymerase chain reaction (RT-PCR) analysis revealed that the transcription of GPX gene of P. dicentrarchii was clearly increased by exposure to 17β-estradiol. The GPX transcription was also clearly increased by exposure to xenoestrogens such as bisphenol A (BPA) and genistein.
Keywords
Philasterides dicentrarchii; Glutathione peroxidase; 17β-estradiol; Bisphenol A; Genistein; Suppression subtractive hybridization; Semiquantitative RT-PCR;
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