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Immune response of olive flounder, Paralichthys oliveceus against B-hemolytic Streptococcus ineae formalin-killed cells  

Jo, Mi-Young (국립수산과학원)
Lee, Deok-Chan (국립수산과학원)
Kim, Jin-Woo (국립수산과학원)
Lee, Ju-Seok (국립수산과학원)
Choi, Hee-Jung (국립수산과학원)
Publication Information
Journal of fish pathology / v.19, no.1, 2006 , pp. 73-82 More about this Journal
Abstract
Streptococcal infections were considered as a serious problem because of significant economic losses in fish farm industry. We evaluated the efficacies of Streptococcus iniae vaccines in olive flounder, Paralichthys olivaceus. The vaccines were prepared from 10% neutral buffered formalin to give a final concentration of 0.3% or 3%, respectively. Fish were immunized by intraperitoneal injection of the experimental vaccines once or twice. Neither of the vaccines gave rise to any significant side effects. The antibody titers of booster immunized groups were significantly higher than those of prime immunized groups with both of the vaccines. According to formalin dosage, significantly increased antibody titers were produced by 3% formalin-killed cells (FKC) at 4weeks and 8weeks after prime and booster vaccination, respectively. Although the different levels of antibody production were showed by the vaccinated fish, the good protection obtained in challenge trials of the both vaccines. Fish immunized with 0.3% FKC once or twice had the relative percent survival (RPS) of 66.7% and 87.5%, respectively. Similarly, fish immunized with 3% FKC once or twice had the RPS of 70.0% and 77.0%, respectively. Further experiments are needed to study not only correlation between the antibody titers and RPS against S. iniae but also the side effects of high dose of formalin on antigenicity.
Keywords
Streptococcus iniae, flounder; Paralichthys olivaceus; formalin-killed cells; safety; immune response;
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