Distribution of Quinolones (Ciprofloxacin, Norfloxacin and Oxolinic acid) after Oral Administration in Carp (Cyprinus carpio)

The concentrations of quinolones (oxolinic acid; OXA, norlloxacin: NRF & ciprofloxacin: CPF) after oral administration of single doses (20 mg/kg B.W.) were investigated in carp (Cyprinus carpio) kept in freshwater at 20-23$^{\circ}C$. The distribution of the drug was studied after treatment. At points timed, from 1 h to 96 hrs after administration, blood (B), liver (L), kidney (K) and muscle (M) from 5 individuals in each group were collected for analyse with microbiological bioassay method. The peak concentrations were measured at 8 h (L), 12 h (B and K) and 24 h (M) after administration regardless of treated drugs. Considerably high concentrations of CPF (13.8-19.6${\mu}g/m{\ell}$) NRF (11.8-16.9${\mu}g/m{\ell}$) and OXA (10.8-13.9 ${\mu}g/m{\ell}$) were revealed during the 24 h. At the last time point of the experiment (96 h), concentrations of all three quinolones were: OXA, 2.3-6.3 ${\mu}g/m{\ell}$ ; NRF, 3.1-4.5 ${\mu}g/m{\ell}$ ; CPF, 3.0-5.5${\mu}g/m{\ell}$ in samples. The concentrations decreased subsequently, indicating a first rapid redistribution, followed by a slow phase of elimination. The steady state was observed in blood (12-36 h), liver (12-96 h) and muscle (36-96 h) after the initiation of treatment with OXA. Concerning the compartmental concentrations, (L, K. and M/B concentration ratio), the fluctuation of the ratio was founded at different time points, among drugs. For CPF, highest tissue ratios were prolonged in the order of L>K>M (0.65-1.2/0.82-0.93/1.0-1.7) during the experiments. On the other hand, NRF presented L>K>M (0.65-1.3/0.86-1.0) till 24 h, but L>M>K (0.89-1.26) at 36-96 h. OXA showed L>K>M (0.95-2.1) at 1-8 h, M>K>L (0.51-1.0) at 12-36 hand M>L>K (1.0-2.3) at 48-96 h, respectively.

잉어에 있어서 Quinolones (Ciprofloxacin, Norfloxacin 및 Oxolinic acid)의 경구투여에 따른 장기내 분포상

본 실험에서 잉어를 대상으로 CPF, NRF 및 OXA등의 약제를 어체당 20 mg/kg 현탁액을 경구투여 후에 1-96 시간 경시적으로 약물의 동태를 조사 하였다. 각 약제별 최고장기농도도달시간은 간장 (8 h), 혈액 (12 h), 신장(12 h) 및 근육 (24 h) 이었다. 각 약제별 최대농도는 CPF (13.8-19.6 ${\mu}g$/$m\ell$), NRF (11.8-15.9 ${\mu}g$/$m\ell$), 및 OXA (10.8-13.9 ${\mu}g$/$m\ell$)순으로 나타났다. 약제농도는 최고치의 도달 이후 빠르게 소실되다가 48-96 h는 완만한 감소를 보였으며, 이때 장기별 약제농도는 근육 (3.3-6.3 ${\mu}g$/$m\ell$)> 간장 (2.5-5.5 ${\mu}g$/$m\ell$)> 신장 (2.3-4.2 ${\mu}g$/$m\ell$) > 혈장 (2.1-3.0 ${\mu}g$/$m\ell$)순으로 나타났다. 한편, OXA의 경우 다른 약제에 비해서 약물의 정체기가 나타났다. 즉 혈중은 12-36 h;, 고농도저류대 (24 hr; 10.9-8.8 ${\mu}g$/$m\ell$), 근육은 36-96 h에 중간농도대 (60 hr; 8.5-5.8 ${\mu}g$/$m\ell$) 및 간장은 12-96 h에 저농도대 (84 hr; 6.8-3.3 ${\mu}g$/$m\ell$)등이 형성되었다.약제의 혈중농도에 대한 각 장기농도비 (간장;L, 신장;K, 및 근육;M / 혈액;B)는 시간대에 따라 차이를 보였다. CPF는 즉 L>K>M (0.65-1.2/0.82-0.93/1.0-1.7)순으로 시간대별로 차이가 없었다. NRF는 시간대별로 1-8 h 및 12-24 h L>K>M (0.65-1.3/0.86-1.0)순이었으나, 36 h 이후 L>M>K (0.89-1.26)순이었다. OXA의 있어서는 1-8 h에는 L>K>M (0.95-2.1), 12-36에는 M>K>L (0.51-1.0) 및 48-96 h에는 M>L>K (1.0-2.3)으로 나타났다.