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Pathological study on experimental hemorrhagic and hemolytic anemia in Nile tilapia, Oreochromis niloticus  

Song, Na-Young (Department ofAquatic Life Medicine, College of Fisheries Science, Pukyong National University)
Choi, Hye-Jeong (Department ofAquatic Life Medicine, College of Fisheries Science, Pukyong National University)
Lee, Mu-Kun (Department ofAquatic Life Medicine, College of Fisheries Science, Pukyong National University)
Huh, Min-Do (Department ofAquatic Life Medicine, College of Fisheries Science, Pukyong National University)
Publication Information
Journal of fish pathology / v.18, no.3, 2005 , pp. 259-268 More about this Journal
Abstract
This study was conducted to know the differences in pathology between artificially induced hemorrhagic anemia (EHA) and hemolytic anemia (ELA) during the anemic and recovery course, using Nile tilapia (Oreochromis niloticus). EHA were induced by repeated bleedings with a volume of about 1% of body weight through caudal vein. ELA were induced by intraperitoneal injections of 1% phenylhydrazine for 2 times. A period of 16 to 49th day was arbitrarily taken as a recovery phase. There were no prominent clinical signs and gross findings except for pale gills during the anemic state of both. Characteristic erythrocytes with a weekly stained cytoplasm started appearing on the 12th day in both and were still noted on the 49 and 20th day respectively in EHA and ELA. EHA and ELA were normocytic hypochromic and macrocytic normochromic in type respectively, although both were normocytic hypochromic during the recovery phase. In liver, fatty degeneration around central vein on the 12-38th day in EHA and hyaline degeneration around central vein on the 12-26th day in ELA were found. In head kidney, increased hemopoiesis was observed on the 12-26th day in EHA and on the 2-12th day in ELA, and macrophages engulfing erythrocytes were observed on the 16-38th day in EHA and on the 2-12th days in ELA. In spleen, activated ellipsoids on the 12-26th day in EHA. and on the 2-20th day in ELA. In ELA, severe accumulation of hemosiderin in both spleen and head kidney were constantly noted from the 2-49th day. On the 49th, Ht was recovered but Hb was still lower than that of control in both anemia.
Keywords
Anemia; Recovery; Nile tilapia; Oreochromis niloticus; Phenylhydrazine;
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