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Histopathological changes of epithelium following the exposure of N-methyl-N-nitro-N-nitrosoguanidine in Manila clam, Ruditapes philippinarum  

Lee, Mu-Kun (Department of Aquatic Life Medicine, College of Fisheries Science, Pukyong National University)
Huh, Min-Do (Department of Aquatic Life Medicine, College of Fisheries Science, Pukyong National University)
Publication Information
Journal of fish pathology / v.18, no.3, 2005 , pp. 247-258 More about this Journal
Abstract
To understand responses of bivalve tissues to various injurious agents, histopathological observation was done in the Manila clam, Ruditapes hilippinarum, during 240 hours after an intramuscular injection of N-methyl-N-nitro-N-nitrosoguanidine (MNNG). Some histopathological changes were observed with the epithelia of stomach, midgut, hindgut, digestive diverticula and gonads after injecting MNNG. The earliest degenerative changes were recognized in the epithelium of midgut 12 hours after the injection. Partial or complete, epithelial destruction was constantly accompanied by the massive infiltration of a mononuclear, necrotic cell group including hemocytes. At 144 hours later, gonads showed the necrotic desquamation of their germinal epithelia. Nearly all of the infiltrated cells within the destructive epithelium were suggested to be hemocytes, from the binding property with a hemocyte-specific lectin, Ricinus communis (RCA-1). From these results it was concluded that the epithelium of digestive system is vulnerable to parenteral MNNG and hemocytes might be deeply involved in this MNNG-induced destruction of the digestive epithelium.
Keywords
Manila clam; Ruditapes philippinarum; Hemocyte; Histopathology; N-methyl-N-nitro-N-nitrosoguanidine;
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