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Vibrios Isolated from Diseased Marine Culturing Fishes in Korea  

Kim, Su-Mi (Institute of Fisheries Science, Pukyong National University)
Won, Kyung-Mi (Institute of Fisheries Science, Pukyong National University)
Woo, Sung-Ho (Department of Aquatic Life Medicine, Pukyong National University)
Li, Hua (Department of Aquatic Life Medicine, Pukyong National University)
Kim, Eun-Jeon (Department of Aquatic Life Medicine, Pukyong National University)
Choi, Kwang-Jin (Department of Aquatic Life Medicine, Pukyong National University)
Cho, Mi-Young (Pathology Division, NFRDI)
Kim, Myung-Suk (Pathology Division, NFRDI)
Park, Soo-Il (Department of Aquatic Life Medicine, Pukyong National University)
Publication Information
Journal of fish pathology / v.18, no.2, 2005 , pp. 133-146 More about this Journal
Abstract
A bacteriological survey in maricultured fish farms was conducted in Korea from 2002 to 2004. A total number of 166 Vibrio isolates were collected from diseased fishes, including olive flounder (133 isolates), black rock fish (8 isolates), red sea bream (6 isolates), shrimp (5 isolates), black sea bream (4 isolates), abalone (3 isolates) and other fishes (7 isolates). All isolates were phenotypically characterized and then groups were obtained using the traditional biochemical test. Representative isolates of each group were genotypically characterized with sequencing the 16S rRNA genes or 16S-23S intergenic space genes. Above 14 species of Vibrio were identified as V. ichthyoenteri (45 strains), V. alginolyticus (34 strains), V. harveyi (32 strains), Ph. damselae subsp. damselae (Formerly V. damsela, 10 strains), V. campbellii (6 strains), V. costicola-like (6 strains), V. fisheri (5 stains), V. fluvialis (4 strains) and others Vibrio sp. (24 strains) by combining of biochemial and genetic characteristics.
Keywords
Vibrios; marine fishes; V. ichthyoenteri; V. alginolyticus; V. harveyi; Ph. damselae subsp. damselae;
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1 Amann, R. I., Ludwing, W. and Schleifer, K. H.: Phylogenetic identification and in situ detection of individual microbial cells without cultivation. Microbiol. Rev. 59: 143-169, 1995
2 Eddy, S. D. and Jones, S. H.: Microbiology of summer flounder Paralichthys dentatus finger-ling production at a marine fish hatchery. Aquaculture, 211: 9-28, 2002   DOI   ScienceOn
3 Egidius, E.: Vibriosis: Pathogenicity and pathology. A Review. Aquaculture, 67: 15-28,1987   DOI   ScienceOn
4 Farmer, J. J. III and Hickman-Brenner, F. W.: The genera Vibrio and Photobacterium. p. 2952-3011. In A Balows (ed), The Prokaryotes, 2nd ed. Springer-Verlag, New York, 1992
5 Hameed, A. S. S. and Rao, P. V: Studies on the chemical control of a Vibrio campbellii-like bacterium affecting hatchery-reared Penaeus indicus larvae. Aquaculture, 127: 1-9,1994   DOI   ScienceOn
6 Ishimaru, K., Akagawa-Matsushita, M. and Muro-ga, K.: Vibrio ichthyoenteri sp. nov., a pathogen of Japanese flounder (Paralichthys olivaceus) larvae. Int. J. Syst. Bacteriol., 46: 155-159,1996   DOI
7 Kang, B. J.: A study on the characteristics of bacteria isolated from cultured flounder (Paralichthys olivaceus) showing disease symptoms in Jeju area of Korea. Dactor's Thesis. Cheju National University, 2003
8 Krawiec, S. and Riley, M.: Organization of the bacterial chromosome. Microbiol. Rev. 54: 502-539,1990
9 Lee, K. K., Yu, S. R., Chen, F. R., Yang, T. I. and Liu, R C: Virulence of Vibrio alginolyticus isolated from diseased tiger prawn, Penaeus monodon. Curr. Microbiol., 32: 229-231, 1996   DOI   ScienceOn
10 Love, M., Teebken-Fisher, D. T., Hose, J. E., Farmer III, J. J., Hickman, F. W. and Fanning, G. R.: Vibrio damsela, a marine bacterium, causes skin lesions on the damselfish Chromis punctipinnis. Science, 214: 1139-1140, 1981   DOI   PUBMED   ScienceOn
11 Muroga, K., Jo, Y, and Masmura, K.: Vibrio ordalii isolated from diseased ayu (Plecoglossus altivelis) and rockfish (Sebastes schlegeli). Fish Pathol., 21: 239-242,1986b   DOI
12 Muroga, K. and Tatani, M.: Isolation of Vibrio anguillarum from juvenile red sea-bream (Pagrus major). Fish Pathol., 16: 211-214, 1982   DOI
13 Schiewe, M. H., and Crosa, J. H.: Molecular characterization of Vibrio anguillarum biotype 2. Can. J. Microbiol. 27: 1011-1018,1981   DOI
14 Sunaryanto, A. and Mariam, A.: Occurrence of a pathogenic bacteria causing luminescence in penaeid larvae in Indonesian hatcheries. Bull. Brackishwater Aquacult. Dev. Cen., 8: 105-112,1986
15 Yii, K. C, Yang, T. I. and Lee, K. K.: Isolation and characterization of Vibrio carchariae, a causative agent of gastroenteritis in the groupers, Epinephelus coioides. Curr. Microbiol., 35: 109-115,1997   DOI   ScienceOn
16 Schiewe, M. H., Trust, T. J. and Crosa, J. H.: Vibrio ordalii sp. nov. a causative agent of vibriosis in fish. Current Microbiology, 6: 343-348, 1981   DOI
17 Lamas, J., Anadon, R., Devesa, S. and Toranzo, A. E.: Visceral neoplasia and epidermal papillomas in cultured turbot Scophthalmus max-imus. Dis. Aquat. Org., 8: 179-187,1990   DOI
18 Lavilla-Pitogo, C. R., Baticados, M. C. L., Cruz-Lacierda, E. R. and de la Pena, L. D.: Occurrence of luminous bacterial disease of Penaeus monodon larvae in the Philippines. Aquaculture, 91: 1-13,1990   DOI   ScienceOn
19 Muroga, K., Iida, M., Masumoto, H. and Nakai, T.: Detection of Vibrio anguillarum from water. Nippon Suisan Gakkaishi. 52: 641-647, 1986a   DOI
20 Austin, B., Stuckey, L. F., Robertson, P. A. W., Effendi, I. and Griffith, D. R. W.: A probiot-ic strain of Vibrio alginolyticus effective in reducing disease caused by Aeromonas salmonicida, Vibrio anguillarum and Vibrio ordallii. J. Fish Dis. 18: 93-96,1995   DOI   ScienceOn
21 Pedersen, K., Verdonck, L., Austin, B., Austin, D. A., Blanch, A. R., Grimont, P. A. D., Jofre, J., Koblavi, S., Larsen, J. L., Tiainen, T., Vigneulle, M. and Swings, J.: Taxonomic evidence that Vibrio carchariae Grimes et al. 1985 is junior synonym of Vibrio harveyi (Johnson and Shunk 1936) Baumann et al. 1981. Int. J. Syst. Bacteriol., 48: 749-758, 1998   DOI   ScienceOn
22 Soffientino, B., Gwaltney, T., Nelson, D. R., Speck-er, J. L., Mauel, M. and Gomez-Chiarri, M.: Infectious necrotizing enteritis and mortality caused by Vibrio carchariae in summer flounder Paralichthys dentatus during intensive culture. Dis. Aquat. Org., 38: 201-210, 1999   DOI   PUBMED
23 Muroga, K., Tanasoomwang, V. and Momoyama, K.: Vibrio anguillarum infection in juveniles of tiger puffer (Takifugu rubripes). Fish Pathol, 22: 29-30, 1987   DOI
24 Sedano, J., Zorrilla, I., Morinigo, M. C, Vidaurreta, A., Bordas, M. A. and Borrego, J. J.: Microbial origin of the abdominal swelling affecting farmed larvae of gilt-head seabream, Sparus aurata L. Aquaculture Res. 27: 323-333, 1996   DOI   ScienceOn
25 Smith, S. K., Sutton, D. C, Fuerst, J. A. and Reichelt, J. L.: Evaluation of the genus Lis-tonella and reassignment of Listonella damsela (Love et al.). MacDonell and Colewell to the genus photobacterium as Photobacterium damsela comb. nov. with an emended description. Int. J. System. Bacteriol., 41: 529-534,1991   DOI   ScienceOn
26 Rheinheimer, G.: Aquatic Microbiology. Wiley, London, UK, 364 pp, 1992
27 Grisez, L., Reyner, J., Verdonck, L., Swings, J. and Ollevier, E: Dominant intestinal microflora of sea bream and sea bass larvae, from two hatcheries, during larval development. Aquaculture, 155: 387-399, 1997   DOI   ScienceOn
28 Lee, H. G., Kim, H. J. and Kim, I.: Isolation of Vibrio species from cultured flounder (Par-alichthys olivaceus) with ulcers and Ascites in the Southern coast of Korea during the winter season. Kor. Jour. Microbiol. 29: 319-328, 1991
29 Austin, B. and Austin, D. A.: Bacterial fish pathogens. Diseases of farmed and wild fish. Springer-Praxis Publishing. Ltd., United Kingdom, 1999
30 Kelly, M. T, Hickman, F. W. and Farmer, J. J.: Vibrio p. 384-395. In Balows A. (ed.). Mannual of clinical microbiology 5th ed. Amer. Soc. Microbiol., 1991
31 Shin, S. U. and Jung, K. J.: Bacterial flora of East China sea and Yosu costal sea areas: 2. Horizontal distributions of bacteria isolated from the sea area. J. Korean Fish Soc., 29: 17-25, 1996
32 Angulo, L., Lopez, J. E., Lema, C. and Vicente, J. A.: Vibrio pelagius associated with mortalities in farmed turbot, Scophthalmus maximus. Thalassas, 10: 129-133,1992
33 Huq, A. and Colwell, R. R.: Vibrios in the marine and estuarine environments. J. Marine Biotechnol., 3: 60-63, 1995
34 Balebona M. C, Zorrilla, I., Morinigo, M. M. and Borrego, J. J.: Survey of bacterial pathologies affecting farmed gilt-head sea bream (Sparus aurata L.) in southwestern Spain from 1990 to 1996. Auaculture, 166: 19-35, 1998
35 MacFaddin, J. E.: Individual biochemical tests. In: Biochemical tests for identification of medical bacteria, 3rd ed., pp. 1-456. Lippincott Williams and Wikins, Philadelphia, 2000
36 Alasina, M. and Blanch, A. R.: Improvement and update of a set of keys for biochemical identification of Vibrio species. J. Appl. Bacteriol., 77: 719-721,1994   DOI
37 Kim, D. H., Han, H. J., Kim, S. M., Lee, D. C. and Park, S. L: Bacterial enteritis and the development of the larval digestive tract in olive flounder, Paralichthys olivaceus (Temminck & Schlegel). J. Fish Dis., 27: 497-505,2004   DOI   ScienceOn
38 Nicolas J. L., Corre, S., Gauthier, G., Rober, R. and Ansquer, D.: Bacterial problems associated with Scallop Pacten maximus larval culture. Dis. Aquat. Org., 27: 67-76, 1996   DOI
39 Lee H. G., Son, B. W. and OH, M. J.: Microbial flora in ascitic cultivated olive flounder (Paralichthys olivaceus) in Koe-je Island in Korea during 2002-2003. Kor. Jour. Microbiol. 40: 133-138, 2004
40 Thompson, J. D., Higgins, D. G. and Gibson, T. J.: Clustal W : Improving the sensitivity of pro-gressive multiple sequence alignment through sequence weighing, positions specific gap penalities and weight matrix choice, Nucleic Acids Res., 22: 4673-4680, 1994   DOI   ScienceOn
41 Yamanoi, H., Momoyama, K., Yasunobu, H. and Muroga, K.: Vibrio anguillarum infection in flounder (Paralichthys olivaceus) fingerings. Fish Pathol., 23: 69-70,1988   DOI
42 Lopez J. E. and Angulo, L.: Survival of Vibrio splendius biotype I in seawater. Bulletin of the European Association of Fish Pathologists. 15: 70-72,1995
43 Euzeby, J. P.: List of bacterial names with standing in nomenclature: a folder available on the Internet (URL: http://www.bacterio.cict.fr/). Int. J. Syst. Bacteriol. 47: 590-592, 1997   DOI   ScienceOn
44 Holt, J. G., Krieg, N. R., Sneath, P. H. A., Staley, J. T. and Williams, S. T: Bergey's manual of determinative bacteriology 9th ed., p. 260-289. Williams and Wilkins Co. Baltomore, Maryland, USA, 1994
45 Bryant, T. N., Lee, J. V., West, P. A. and Colwell, R. R.: Numerical classification of species of Vibrio and related genera. J. Appl. Bacteriol., 61:437-467, 1986   DOI
46 Zorrilla I., Chabrillon, M., Arijo, S., Diaz-Rosales, R, Martinez-Manzanares, Balebona, M. C. E. and Morinigo, M. A.: Bacterial recovered from diseased cultured gilthead sea bream (Spams aurata L.) in Southwestern Spain. Aquaculture, 218: 11-20, 2003   DOI   ScienceOn
47 Gurtler, V and Stanisich, V A.: New approaches to typing and identification of bacteria using the 16S-23S rDAN spacer region. Microbiology, 142: 3-6, 1996   DOI   ScienceOn
48 Chen, D., Hanna, P. J., Altmann, K., Smith, A., Moon, P. and Hammond, L. S.: Development of monoclonal antibodies that identify vibrio species commonly isolated from infections of humans, fish and shellfish. Appl. Environ. Microbiol., 58: 3694-3700, 1992
49 Lightner, D. V.: Disease of cultured penaeid shrimp. In: McVey, J. P. (Ed.), CRC Handbook of Mariculture. Crustacean Aquaculture. CRC Press, Boca Raton, FL, pp. 393-486, 1993
50 Bacticados, M. C. L., Lavilla-Pitogo, C. R., Cruz-Lacierda, E. R., de la Pena, L. D. and Sunaz, N. A.: Studies in chemical control of luminous bacteria Vibrio harveyi and Vibrio splendidus isolated from diseased Penaeus monodon larvae and rearing water. Dis. Aquat. Org., 9: 133-139,1990   DOI