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Prevalence and Detection of Perkinsus sp. infection in the Manila clams, Ruditapes philippinarum  

Park, Sung-Woo (Dept. of Aquatic Life Medicine, Kunsan National University)
Lee, Kyung-Hee (Fisheries Management Division, Mokpo Regional Maritime Affairs and Fisheries Office)
Choi, Dong-Lim (Pathology Team, National Fisheries Research and Development Institute)
Publication Information
Journal of fish pathology / v.18, no.1, 2005 , pp. 49-58 More about this Journal
Abstract
Hemacolor stain, histopathological observation and FTM incubation assay were applied to detect Perkinsus sp. infection in Manila clams (Ruditapes philippinarumi taken from culture beds at Tean and Gochang from March 2002 to August 2003. The prevalency was 100% in the clams from Gochang and 20-70% from Tean. Of the three methods, histopathological observation was the most effective to detect the infection. And the parasites was most abundant in gills. When PCR assay was applied to detect Perkinsus sp. for four species of Mollusc such as manila clam, surf clam (Mactra veneriformis), oyster (Crassostrea gigas) and Thomas' rapa whelk (Rapana venosa) taken from Tean and Gochang from April to July 2004, the parasites were detected from all the species at the infection rates of 95%, 62%, 46.9% and 10% in that order. The infection rate was much higher in the species burrowing in the bottom sediments than those attaching on the tidal rock. The results suggest that Perkinsus sp. may affect almost all the molluscs inhabiting western coastal areas of Korea.
Keywords
Ruditapes philippinarum; Perkinsus sp; PCR;
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