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Differentiation of three scuticociliatosis causing species in olive flounder (Paralichthys olivaceus) by multiplex PCR  

Kim, Sung Mi (부경대학교)
Lee, Eun Hye (부경대학교)
Kim, Ki Hong (부경대학교)
Publication Information
Journal of fish pathology / v.17, no.2, 2004 , pp. 145-149 More about this Journal
Abstract
The definitive identification of ciliate species by morphological characteristics relies on time-consuming and laborious staining techniques. Therefore, in this study, we discriminated 3 scuticociliatosis causing species - Pseudocohnilembus persalinus, Uronema marinum and Philasterides dicentrarchi - in cultured olive flounder by multiplex PCR. The multiplex PCR based on the species-specific amplification of small subunit ribosomal RNA (SS rRNA) gene sequence enabled us to distinguish the 3 scuticociliate species in a simple and rapid manner, even in the sample containing the three species simultaneously. These data suggest that the multiplex PCR strategy would make it possible to avoid the cumbersome and time-consuming procedures of morphological analysis for the definitive identification of scuticociliates.
Keywords
Pseudocohnilembus persalinus, Uronema marinum, Philasterides dicentrarchi, Scuticociliatosis, Olive flounder, SS rRNA gene, Multiplex PCR
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