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Immune responses th the vaccines of viral systemic necrosis of carp virus (VSNCV) of comom carp, Cyprinus carpio L.  

Jo, Mi-Yeong (진해내수면연구소)
Son, Sang-Gyu (국립수산과학원)
Kim, Lee-Cheong (국립수산과학원)
Kim, Jin-U (국립수산과학원)
O, Myeong-Ju (여수대학교)
Jeong, Seong-Ju (여수대학교)
Park, Su-Il (부경대학교)
Publication Information
Journal of fish pathology / v.16, no.3, 2003 , pp. 175-181 More about this Journal
Abstract
VSNC is a viral disease causing significant economic losses in cultured carp Ciprinus carpio L. in Korea. Carps were immunized with prepared vaccines against VSNCV and examined specific and nonspecific immune responses. Carps were injected by O.2㎖ of formalin-killed vaccine (FKV), heat-killed vaccine (HKV) or E-MEM, respectively and dealt with boost with same way two weeks later. The lysozyme activity of serum and chemiluminescent reponses of head-kidney leucocytes showed increased responses during 2-7 days post-first injection (pfi) and post-boost (pb) in the vaccinated fish, and then decreased to the level of control. As measured by ELISA, vaccinated groups showed a significant increase in VSNCV-specific serum antibodies between 2 weeks pfi and 6weeks pb with a peak at 2 weeks pb. Results of the virus challenge showed that the fish vaccinated with FKV have induced protective immunity, while HKV injection hardly provided protection.
Keywords
VSNCV, serum antibody, lysozyme, chemiluminescent reponse
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1 노용길, 이배익, 김광석, 강언종, 김이청, 손재경 : 내수면 양식의 개요. pp.3-7. 진해내수면연구소, 1997
2 Jolles, P. and Jolles, J. : What's new in lysozyme research? Always model system, today as yesterday. Mol. Biochem., 63:165-189, 1984
3 Murphy, J. W., Schafer, F., Casadevall, A. and Adesina, A. : Antigen-induced protective nonprotective cell-mediated immune components against Crytococcus neoformans. Infec. Immun., 66(6):2632-2639, 1998
4 Yoan, R., Casadevall, A., Oh, J. and Scharff, M. D. : T cells cooperate with passive antibody to modify Crytococcus neoformans infection in mice. Proc. Natl. Acad. Sci. USA, 94:2483-2488, 1997   DOI   ScienceOn
5 Paulsen, S. M., Lunde, H., Engstad, R. E. and Robertsen, B. : In vivo effects of $\beta$-glucan and LPS on regulation of lysozyme activity and mRNA expression in Atlantic salmon (Salmo salar L.). Fish & shellfish Immunol., 12:1-16, 2002
6 Khakpour, F. R. and Murphy, J. W. : Characterization of a third-order suppressor T cell(Ts3) induced by crypococcal antigen(s). Infec. Immun., 55:1657-1662, 1987
7 Sveinbjørnsson, B., Olsen, R. and Paulsen, S. : Immunocytochemical localization of lysozyme in intestinal eosinophilic granular cells (EGCs) of Atlatic salmon, Salmo salar L. J. Fish Dis., 19:349-355, 1996   DOI   ScienceOn
8 Agneil, L. D. : An assessment of passive transfer of immunity to infectious pancreatic necrosis virus (IPNV) in trout. M. S. thesis. American University, Washington, D. C., 1975
9 Desvignes, L., Quentel, C., Lamour, F. and LE Ven. A. : Pathogenesis and immune response in Atlantic salmon (Salmo salar L.) parr experimentally infected with salmon pancreas disease virus (SPDV). Fish & Shellfish Immunol., 12:77-95, 2002   DOI   ScienceOn
10 Ellis, A. E. : Lysozyme assays. In 'Techniques in fish immunology'. Stolen, J. S. Flecher, T.C., Anderson, D.P., Roberson, B.S. and van Muiswinkel, W.B., eds., pp. 101-103, Fair Haven, NJ:SOS Publication, 1990
11 Fletcher, T. C. and White, A. : The lysozyme of the plaice Pleuronecyes platessa L. Comp. Biochem. Physiol., 55:207-210, 1976   DOI   ScienceOn
12 Bootland, L. M., Dobos, P. and Stevenson, R. M. W. : Immunization of adult brook trout, Salvelinus fontinalis (Mitchell), fail to prevent the infectious pancreatic necrosis virus (IPNV) carrier state. J. Fish Dis. 18:449-458,1995   DOI   ScienceOn
13 Yamamoto, T. : Infectious pancreatic necrosis (IPN) virus carriers and antibody production in a population of rainbow trout (Salmo gairdneri). Can. J. Microbiol. 21:1343-1347, 1975   DOI   ScienceOn
14 Alexander, J. B. and Ingram. G. A. : Noncellular nonspecific defence mechanisms of fish. Ann. Rev. Fish Dis., 2:249-280, 1992   DOI   ScienceOn
15 Oh, M.J., Jung, S. J., Choi, T. J., Kim, H. R., Rajendran, K. V., Kim, Y. J., Park, M. A. and Chun., S. K. : A viral disease occurring in cultured carp in Korea. Fish Pathol., 36:147-151, 2001   DOI
16 Buchanan, K. L. and Murphy, J. W. : Characterization of cellular infiltrates and cytokine production during the expression phase of the anticrytococcal delayed-type hypersensitivity response. Infec. Immun.61:2854-2865,1993
17 Reno, P. W. : Qualitative and quantitative aspects of the infectious pancreatic necrosis virus (IPN) carrier state in trout. Ph. D. thesis. University of Guelph, Ontario, Canada, 1976
18 Smail, D. A. and Munro, A. L. S. : Infectious pancreatic necrosis virus persistence in farmed Atlantic salmon (Salmo salar). In Fish and Shellfish Pathology. A. E. Ellis, ed., pp.277-288. London : Academic Press, 1985
19 Ellis, A. E. : Innate host defense mechanisms of fish against viruses and bacteria. Dev. Comp. Immunol.,25:827-839, 2001   DOI   ScienceOn