Browse > Article

Establishment and characterization ofnew cell line derived from black seabream (Acanthopagrus schlegeli)  

Im, Eun-Young (Department of Aqualife Medicine, Yosu National University)
Kang, Min-Sue (Department of Aqualife Medicine, Yosu National University)
Oh, Myung-Joo (Department of Aqualife Medicine, Yosu National University)
Jung, Tae-Sung (College of Veterinary Medicine, Gyeongsang National University)
Jung, Sung-Ju (Department of Aqualife Medicine, Yosu National University)
Publication Information
Journal of fish pathology / v.16, no.3, 2003 , pp. 165-173 More about this Journal
Abstract
A stable cell line, BSBS (black seabream spleen), was established from the cells in spleen of black seabream, Acanthopagrus schlegeli, and characterized. Subculture maintained more than 60 passages and mophologically, BSBS cell was epithelioid cell. The cells grew optimally at 20℃ in Leibovitz's L-15 medium supplemented with 10% fetal bovine serum with incubation temperature of 20℃. BSBS cells supported the growth of marine birnavirus (MABV Y-6), chum salmon reovirus (CSV), spring viremia of carp virus (SVCV) and hirame rhabdovirus (HIRRV). Thus, the new cell line may be useful for studying wide range of fish viruses.
Keywords
new cell line; black seabream; BSBS; virus susceptibility;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Bols, N.C. and Lee, E.L.J.: Technology and uses of cell cultures from tissues and organs of bony fish. Cytotechnology, 6: 163-187, 1991   DOI
2 Chang, S.F., Ngoh, G.H., Kueh, L.F.S., Qin, Q.W., Chen, C.L., Lam, T.J. and Sin, Y.M.: Development of a tropical marine fish cell line from Asian seabass (Lates calcarifer) for virus isolation. Aquaculture, 192: 133-145, 2001   DOI
3 Holt, R. and Rohovec, J.: Anaemia of coho salmon in Oregon. Fish Health Section. Am. Fish Soc. Newsletter, 12: 4, 1984
4 Hsu, T.C.: Karyology of cells in culture. In: P.K. Kruse., Jr. and M.K Patterson, Jr (ed.) Tissue culture methods and applications, Academic Press, New York, pp. 764-767, 1973
5 Iida, Y., Masumura, K., Nakai, T., Sorimachi, M and Matsuda, H.: A viral disease in larvae and juveniles of the Japanese flounder Paralichthys olivaceus. J. Aquat. Anim. Health, 1: 7-12, 1989   DOI
6 Iwamoto, T., Nakai, T., Mori, K., Arimoto, M and Furusawa, I.: Cloning of the fish cell line SSN-1 for piscine nodaviruses. Dis. Aqua. Organ., 43: 81-89, 2000   DOI
7 Jung, S.J. and Oh, M.J.: Iridovirus-like infection of striped beakperch Oplegnathus fasciatus (Temminck et Schlegel) associated with high mortality in a south coastal area of the Korean peninsula. J. Fish Dis., 23: 223-226, 1999
8 Lai, Y-S., Murali, S., Ju, H-Y., Wu, M-F., Guo,, I-C., Chen, S-C., Fang, K. and Chang, C-Y. Two iridovirus-susceptible cell lines established from kidney and liver of grouper, Epinephelus awoara (Temminck & Schlegel), and partial characterization of grouper iridovirus. J. Fish Dis. 23: 379-388, 2000   DOI   ScienceOn
9 Mori, K., Nakai, T., Muroga K., Arimoto, M., Musiake, K and Furisawa, I.: Properties of a new virus belonging to Nodaviridae found in striped jack, Pseudocaranx dentex, with nervous necrosis. Virology. 187: 368-371, 1992   DOI   ScienceOn
10 Fryer J.L. and Lannan C.N: Rickettsial and chlamydial infections of freshwater and marine fishes, bivalves, and crustaceans. Zool. Stud., 33: 95-107, 1994b
11 Kusuda, R., Nishi, Y., Hosono, N. and Suzuki, S.: Serological comparison of birnavirus isolated from several species of marine fish in south west Japan. Fish Pathol., 28: 91-92, 1993   DOI   ScienceOn
12 Fernandez-Puentes C. and Figueras A.: Epithelial cell line from turbot (Scophthalmus maximus L.). In Vitro Cell. Dev. Biol. Anim., 32: 391-393, 1995   DOI   ScienceOn
13 Bahich, H. and Borenfreund, E.: Cytotoxicity and genotoxicity assays with cultured fish cells: a review. Toxicol. In Vitro, 3: 91-100, 1991
14 Wolf, K. and Quimby, M.C.: Established eurythermic line of fish cells in vitro. Science 135: 1065-1066, 1962   DOI
15 Yoshimizu, M., Nomura, T., Awakura, T., Ezura, Y. and Kimura, T.: Prevalence of pathogenic fish viruses in anadromous masu salmon (Oncorhynchus masou) in the northern part of Japan, 1976-1987. Physiol. Ecl. Jpn. (Special Volume) 1: 559-576, 1989
16 Wolf, K., Gravell, M. and Malsberger, R.G.: Lymphocystis virus: isolation and propagation in centrarchid fish cell lines. Science, 151: 1004-1005, 1966   DOI
17 Fryer J.L. and Lannan C.N: Three decades of cell culture: a current listing of cell lines derived from fishes. J. Tissue Cult. Methods, 10: 57-94, 1994a
18 Smail, D.A. and Egglestone, S.I.: Virus infections of marine fish erythrocytes: electron microscopical studies on the blenny virus. Fish Dis., 3: 47-54, 1980   DOI
19 Freshney R.I.: Culture of animal cells. In: Freshney, R.I. (ed.) A manual of basic technique, Wiley-Liss, New York, pp. 387-389, 1994
20 Lannan, C.N., Winton, J.R. and Fryer J.L.: Fish cell lines: establishment and characterization of nine cell lines from salmonids. In vitro, 20: 107-114, 1984
21 Winton, J.R., Lannan, C.N., Fryer, J.L. and Kimura ,T.: Isolation of a new reovirus from chum salmon. Fish Pathol., 15: 155-162, 1981   DOI
22 Reno, P.W., Philippon-Fried, P.H. and Nicholson, B.L.: Ultrastructural studies of piscine erythrocytic necrosis(PEN) I Atlantic herring (Clupea harengus) J. Fish Res. Board Can., 35: 148-154, 1978   DOI
23 Tong, S.L., Miao, H.Z. and Li, H.: Three new continuous fish cell lines of SPH, SPS and RSBF derived from sea perch (Lateolabrax japonicus) and red sea bream (Pagrosomus major). Aquaculture, 169:143-151, 1998   DOI
24 Kasai, H. and Yoshimizu, M: Establishment of two Japanese flounder embryo cell lines. J. Fac. Fish. Sci. Hokkaido Univ., 52: 67-70, 2001
25 Oh, M.J., Jung, S.J., Kim, S.R., Rajendran, K.V., Kim, Y.J., Choi, T.J., Kim, H,R, and Kim, J.D.: A fish nodavirus associated with mass mortality in hatchery- reared red drum Sciaenops ocellatus. Aquaculture, 211: 1-7, 2002   DOI
26 Tung L-C., Chen S-N. and Kou G-H.: Three cell lines derived from spleen and kidney of black porgy (Acanthopagrus schlegeli). Fish Pathol., 26: 109-117, 1991   DOI
27 Fryer, J.L., Lannan, C.N., Garces, L.H., Larenas, J.J. and Smith, P.A.: Isolation of a rickettsiales-like organism from diseased coho salmon (Oncorhynchus kisutch) in Chile. Fish Pathol., 25: 107-114, 1990   DOI
28 Inouye, K., Yamano, Y., Maeno, Y., Nakajima, K., Matsuoka, M., Wada, Y. and Sorimachi, M.: Iridovirus infection of cultured red seabream Pagrus major. Fish Pathol., 27: 19-27, 1992   DOI
29 Lai, Y-S., John, J.A.C., Lin, C-H., Guo, I-C., Chen, S-C., Fang, K., Lin, C-H. and Chang, C-Y.: Establishment of cell lines from a tropical grouper, Epinephelus awoara (Temminck & Schlegel), and their susceptibility to grouper Irido- and nodaviruses. J. Fish Dis., 26: 31-42, 2003   DOI   ScienceOn
30 Lai, Y-S., Murali, S., Chiu, H-C., Ju, H-Y., Lin, Y-S., Chen, S-C., Guo, I-C, Fang, K. and Chang, C-Y. Propagation of yellow grouper nervous necrosis virus (YGNNV) in a new nodavirus-susceptible cell line from yellow grouper, Epinephelus awoara (Temminck & Schlegel), brain tissue. J. Fish Dis., 24: 299-309, 2001   DOI   ScienceOn
31 Oh, M.J., Jung, S.J., Choi, T.J., Kim, H.R., Rajendran, K.V., Kim, Y.J., Park, M.A., and Chun, S.K. A viral disease occurring in cultured carp Cyprinus carpioin Korea. Fish Pathol., 36: 147-152, 2001   DOI
32 Iida, Y., Nakai, T., Sorimachi, M. and Matsuda, H.: A viral disease of larvae and juveniles of Japanese flounder Paralichthys olivaceus. Dis. Aquat. Org., 10: 59-63, 1991   DOI
33 Chi, S. C., Hu, W.W. and Lo, B. J.: Establishment and characterization of continuous cell line (GF-1) derived from grouper, Epinephelus coioides (Hamilton): a cell line susceptible to grouper nervous necrosis virus (GNNV)., J. Fish Dis., 22: 173-182, 1999   DOI
34 Oh, M.J. and Choi, T.J.: A new rhabdovirus (HRV-like) isolated from cultured Japanese flounder Paralichthys olivaceus. J. Fish Pathol., 11: 129-136, 1998 (Korean)
35 Fernandez, R.D., Yoshimizu, M., Kimura, T., Ezura, Y., Inouye, K. and Takami, I.: Characterization of three continuous cell line from marine fish. J. Aquat. Anim. Health, 5: 127-136, 1993   DOI   ScienceOn